Detects human Flt-3 Ligand in direct ELISAs and Western blots. In direct ELISAs, this antibody does not cross-react with recombinant mouse Flt‑3 Ligand.
Monoclonal Mouse IgG1 Clone # 40406
Protein A or G purified from ascites
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Flt-3 Ligand Thr27-Pro185 Accession # AAA17999
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Recombinant Human Flt‑3 Ligand (Catalog # 308-FK) under non-reducing conditions only
Measured by its ability to neutralize Flt‑3 Ligand-induced proliferation in the BaF3 mouse pro‑B cell line transfected with mouse Flt‑3. Hannum, C. et al. (1994) Nature 368:643. The Neutralization Dose (ND50) is typically 0.02‑0.06 µg/mL in the presence of 5 ng/mL Recombinant Human Flt‑3 Ligand.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Cell Proliferation Induced by Flt‑3 Ligand and Neutralization by Human Flt‑3 Ligand Antibody. Recombinant Human Flt‑3 Ligand (Catalog # 308-FK or 308-FKN) stimulates proliferation in the BaF3 mouse pro‑B cell line transfected with mouse Flt‑3 in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Flt‑3 Ligand (5 ng/mL) is neutralized (green line) by increasing concentrations of Human Flt‑3 Ligand Monoclonal Antibody (Catalog # MAB308). The ND50 is typically 0.02-0.06 µg/mL.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Flt-3 Ligand
Flt-3 Ligand, also known as Flk-2 Ligand, is a transmembrane protein
that can be cleaved to release a soluble cytokine. Flt-3 Ligand binds
Flt-3 and promotes the growth and differentiation of early
hematopoietic progenitor cells.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.