Human Follistatin Antibody

Catalog # Availability Size / Price Qty
Human Follistatin ELISA Standard Curve.
1 Image
Product Details
Supplemental Products

Human Follistatin Antibody Summary

Species Reactivity
Detects human Follistatin in direct ELISAs.
Monoclonal Mouse IgG1 Clone # 85943
Protein A or G purified from ascites
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Follistatin
Accession # P19883
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human Follistatin Monoclonal Antibody (Catalog # MAB6691).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Follistatin DuoSet ELISA Kit (Catalog # DY669) for convenient development of a sandwich ELISA or the Human Follistatin Quantikine ELISA Kit (Catalog # DFN00) for a complete optimized ELISA.


Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Human Follistatin ELISA Standard Curve. Recombinant Human Follistatin protein was serially diluted 2-fold and captured by Mouse Anti-Human Follistatin Monoclonal Antibody (Catalog # MAB6691) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Follistatin Monoclonal Antibody (Catalog # MAB6692) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Follistatin

Follistatin (FST) is a secreted glycoprotein that was first identified as a follicle-stimulating hormone inhibiting substance in ovarian follicular fluid (1, 2). Human Follistatin cDNA encodes a 344 amino acid (aa) protein with a 29 aa signal sequence, an N-terminal atypical TGF binding domain, three Follistatin domains that contain EGF-like and kazal-like motifs, and a highly acidic C-terminal tail. The first Follistatin domain (FS1) contains a heparin binding site, while FS1 and FS2 are most critical for activin binding and neutralization (3, 4). In addition to activin, Follistatin regulates bioavailability of many non-TGF-beta members of the TGF-beta superfamily, such as BMP6, BMP7 and myostatin (5). It also regulates hematopoietic stem cell adhesion to fibronectin via FS2, and binds angiogenin via FS2 and FS3 (6, 7). Some Follistatin binding partners will also bind Follistatin-like proteins such as FSL-3 (3, 5, 6). Of three Follistatin isoforms, the full-length mature Follistatin (FST315) is the most abundant and the sole form in plasma, but has lower binding affinity for both activins and heparins than alternative isoforms (5, 8, 9). The acidic tail is missing in the splice variant FST288 which shows the highest affinity for activins, while a partial tail exists in the proteolytically produced FST303, which shows intermediate activin affinity (5, 8, 9). FST315 shares 98% aa identity with mouse, rat, equine and ovine FST, 99% with porcine and 97% with bovine FST. Genetic deletion of Follistatin in mice, or expression of only the FST288 form, is perinatally lethal due to defects of lung, skin and musculoskeletal system (10). Expression of only the FST315 isoform allows survival, with defects in vascularization and female fertility (10).

  1. Shimasaki, S. et al. (1988) Proc. Natl. Acad. Sci. USA 85:4218. 
  2. Thompson, T.B. et al. (2005) Dev. Cell 9:535. 
  3. Sidis, Y. et al. (2005) Endocrinology 146:130. 
  4. Keutmann, H.T. et al. (2004) Mol. Endocrinol. 18:228. 
  5. Sidis, Y. et al. (2006) Endocrinology 147:3586. 
  6. Maguer-Satta, V. et al. (2006) Exp. Cell Res. 312:434.
  7. Gao, X. et al. (2007) FEBS Lett. 581:5505.
  8. Lerch, T.F. et al. (2007) J. Biol. Chem. 282:15930.
  9. Schneyer, A.L. et al. (2004) J. Clin. Endocrinol. Metab. 89:5067.
  10. Lin, S-Y. et al. (2008) Mol. Endocrinol. 22:415.
Entrez Gene IDs
10468 (Human); 14313 (Mouse)
Alternate Names
follistatin isoform FST317; Follistatin; FS; FSActivin-binding protein; FST

Product Datasheets

You must select a language.



No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human Follistatin Antibody

There are currently no reviews for this product. Be the first to review Human Follistatin Antibody and earn rewards!

Have you used Human Follistatin Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review