Human Follistatin-like 1/FSTL1 Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB16941-100
MAB16941-SP
Human  Follistatin‑like 1/FSTL1 ELISA Standard Curve.
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Human Follistatin-like 1/FSTL1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Follistatin‑like 1/FSTL1 in direct ELISAs.
Source
Recombinant Monoclonal Rat IgG2B Clone # 229001R
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Follistatin‑like 1/FSTL1
Met1-Ile308
Accession # Q12841
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
ELISA

This antibody functions as an ELISA capture antibody when paired with Goat Anti-Human Follistatin‑like 1/FSTL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1694).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Follistatin-like 1 DuoSet ELISA Kit (Catalog # DY1694) for convenient development of a sandwich ELISA.

 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Human Follistatin‑like 1/FSTL1 ELISA Standard Curve. Recombinant Human Follistatin‑like 1/FSTL1 protein was serially diluted 2-fold and captured by Rat Anti-Human Follistatin‑like 1/FSTL1 Monoclonal Antibody (Catalog # MAB16941) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Follistatin‑like 1/FSTL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1694) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Follistatin-like 1/FSTL1

Follistatin-like 1 (FSL1 or FSTL1), also known as FRP (follistatin-related protein), Flik (follistatin-like), and TSC-36 (TGF-beta 1-stimulated clone 36), is a secreted 45‑55 kDa extracellular glycoprotein belonging to the BM-40/SPARC/Osteonectin family (1-3). The human FSTL1 cDNA encodes 308 amino acids (aa), including a 20 aa signal sequence, a cysteine-rich Follistatin (EGF- and kazal-like) domain, two apparently non-functional EF-hand calcium-binding motifs, and a von Willebrand Factor C homology domain (1, 3). Mature human FSTL1 shares 94%, 95%, 98% and 99% aa identity with mouse, rat, bovine and equine FSTL1, respectively. FSTL1 was first identified as a TGF-beta-induced protein from a mouse osteoblast cell line (4). It is ubiquitously expressed in early mouse development, but is mainly mesenchymal later in development (5). In humans, FSTL1 is a common rheumatoid arthritis auto-antigen (2). It is reported to be either pro-inflammatory due to promoting inflammatory cytokine secretion, or to prevent autoimmune arthritis by inhibiting matrix metalloproteinase (MMP) and prostaglandin expression (6-9). In muscle and heart, it appears to be protective and promotes endothelial cell functions such as revascularization after ischemia, probably due to promoting expression and activation of the protein kinase AKT1 (10, 11). Cardiac and circulating FSTL1 is generally increased in conditions such as heart failure and acute coronary syndrome (11, 12). FSTL1 also appears to be a tumor suppressor, showing down-regulated expression in many human cancers (4, 14, 15). In vitro, it slows proliferation and MMP-dependent migration, and increases FAS-dependent apoptosis of tumor cell lines (14).

References
  1. Zwijsen, A. et al. (1994) Eur. J. Biochem. 225:937.
  2. Tanaka, M. et al. (1998) Int. Immunol. 10:1305.
  3. Hambrook, H.O. et al. (2004) J. Biol. Chem. 279:11727.
  4. Shibanuma, M. et al. (1993) Eur. J. Biochem. 217:13.
  5. Adams, D. et al. (2007) Gene Expr. Patterns 7:491.
  6. Clutter, S.D. et al. (2009) J. Immunol. 182:234.
  7. Miyamae, T. et al. (2006) J. Immunol. 177:4758.
  8. Kawabata, D. et al. (2004) Arthritis Rheum. 50:660.
  9. Tanaka, M. et al. (2003) Int. Immunol. 15:71.
  10. Ouchi, N. et al. (2008) J. Biol. Chem. 283:32802.
  11. Oshima, Y. et al. (2008) Circulation 117:3099.
  12. Lara-Pezzi, E. et al. (2008) Endocrinology 149:5822.
  13. Widera, C. et al. (2009) Clin. Chem. 55:1794.
  14. Chan, Q.K. et al. (2009) Carcinogenesis 30:114.
  15. Mashimo, J. et al. (1997) Cancer Lett. 113:213.
Entrez Gene IDs
11167 (Human); 14314 (Mouse); 79210 (Rat)
Alternate Names
Flik; FLJ50214; Follistatin-like 1; Follistatin-like protein 1; follistatin-related protein 1; Follistatin-related Protein; FRP; FRPFLJ52277; FSL1; FSTL1; TSC-36

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