Human Follistatin-like 1/FSTL1 Antibody

(3 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human Follistatin-like 1/FSTL1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 15% cross‑reactivity with recombinant mouse FSTL1 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Follistatin-like 1/FSTL1
    Met1-Ile308
    Accession # Q12841
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    Immersion fixed paraffin-embedded sections of human placenta
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Follistatin‑like 1/FSTL1 by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Follistatin‑like 1/FSTL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1694) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Follistatin‑like 1/FSTL1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
Follistatin-like 1/FSTL1 in HeLa Human Cell Line. Follistatin-like 1/FSTL1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human Follistatin-like 1/FSTL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1694) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Follistatin-like 1/FSTL1

Follistatin-like 1 (FSL1 or FSTL1), also known as FRP (follistatin-related protein), Flik (follistatin-like), and TSC-36 (TGF-beta 1-stimulated clone 36), is a secreted 45‑55 kDa extracellular glycoprotein belonging to the BM-40/SPARC/Osteonectin family (1-3). The human FSTL1 cDNA encodes 308 amino acids (aa), including a 20 aa signal sequence, a cysteine-rich Follistatin (EGF- and kazal-like) domain, two apparently non-functional EF-hand calcium-binding motifs, and a von Willebrand Factor C homology domain (1, 3). Mature human FSTL1 shares 94%, 95%, 98%, and 99% aa identity with mouse, rat, bovine, and equine FSTL1, respectively. FSTL1 was first identified as a TGF-beta-induced protein from a mouse osteoblast cell line (4). It is ubiquitously expressed in early mouse development, but is mainly mesenchymal later in development (5). In humans, FSTL1 is a common rheumatoid arthritis auto-antigen (2). It is reported to be either pro-inflammatory due to promoting inflammatory cytokine secretion, or to prevent autoimmune arthritis by inhibiting matrix metalloproteinase (MMP) and prostaglandin expression (6-9). In muscle and heart, it appears to be protective and promotes endothelial cell functions such as revascularization after ischemia, probably due to promoting expression and activation of the protein kinase AKT1 (10, 11). Cardiac and circulating FSTL1 is generally increased in conditions such as heart failure and acute coronary syndrome (11, 12). FSTL1 also appears to be a tumor suppressor, showing down-regulated expression in many human cancers (4, 14, 15). In vitro, it slows proliferation and MMP-dependent migration, and increases FAS-dependent apoptosis of tumor cell lines (14).

  • References:
    1. Zwijsen, A. et al. (1994) Eur. J. Biochem. 225:937.
    2. Tanaka, M. et al. (1998) Int. Immunol. 10:1305.
    3. Hambrook, H.O. et al. (2004) J. Biol. Chem. 279:11727.
    4. Shibanuma, M. et al. (1993) Eur. J. Biochem. 217:13.
    5. Adams, D. et al. (2007) Gene Expr. Patterns 7:491.
    6. Clutter, S.D. et al. (2009) J. Immunol. 182:234.
    7. Miyamae, T. et al. (2006) J. Immunol. 177:4758.
    8. Kawabata, D. et al. (2004) Arthritis Rheum. 50:660.
    9. Tanaka, M. et al. (2003) Int. Immunol. 15:71.
    10. Ouchi, N. et al. (2008) J. Biol. Chem. 283:32802.
    11. Oshima, Y. et al. (2008) Circulation 117:3099.
    12. Lara-Pezzi, E. et al. (2008) Endocrinology 149:5822.
    13. Widera, C. et al. (2009) Clin. Chem. 55:1794.
    14. Chan, Q.K. et al. (2009) Carcinogenesis 30:114.
    15. Mashimo, J. et al. (1997) Cancer Lett. 113:213.
  • Entrez Gene IDs:
    11167 (Human); 14314 (Mouse); 79210 (Rat)
  • Alternate Names:
    Flik; FLJ50214; Follistatin-like 1; Follistatin-like protein 1; follistatin-related protein 1; Follistatin-related Protein; FRP; FRPFLJ52277; FSL1; FSTL1; TSC-36
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. Follistatin like-1 aggravates silica-induced mouse lung injury
    Authors: Y Fang, S Zhang, X Li, F Jiang, Q Ye, W Ning
    Sci Rep, 2017;7(1):399.
    Species: Human
    Sample Type: Serum
    Application: ELISA Capture
  2. Follistatin-like protein 1 enhances NLRP3 inflammasome-mediated IL-1beta secretion from monocytes and macrophages.
    Authors: Chaly Y, Fu Y, Marinov A, Hostager B, Yan W, Campfield B, Kellum J, Bushnell D, Wang Y, Vockley J, Hirsch R
    Eur J Immunol, 2014;44(5):1467-79.
    Species: Human
    Sample Type: Tissue Homogenates
    Application: WB
  3. Tumor suppressor effect of follistatin-like 1 in ovarian and endometrial carcinogenesis: a differential expression and functional analysis.
    Authors: Chan QK, Ngan HY, Ip PP, Liu VW, Xue WC, Cheung AN
    Carcinogenesis, 2009;30(1):114-21.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: WB
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