Human FoxO1/FKHR Antibody

Catalog #: AF5939 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5939
AF5939-SP

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Detection of Human FoxO1/FKHR by Western Blot.

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All FoxO1/FKHR Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human FoxO1/FKHR Antibody Summary

Species Reactivity

Human

Specificity

Detects human FoxO1/FKHR in Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human FoxO1/FKHR
Ala353-Gly655
Accession # Q12778

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Simple Western

10 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human FoxO1/FKHR by Western Blot. Western blot shows lysates of 293T human embryonic kidney cell line and JEG-3 human epithelial choriocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human FoxO1/FKHR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5939) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FoxO1/FKHR at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western

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Detection of Human FoxO1/FKHR by Simple Western^TM. Simple Western lane view shows lysates of 293T human embryonic kidney cell line, loaded at 0.2 mg/mL. A specific band was detected for FoxO1/FKHR at approximately 93 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human FoxO1/FKHR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5939) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FoxO1/FKHR

FoxO1, also called FKHR (forkhead in rhabdomyosarcoma), is a 655 amino acid (aa), 70 kDa, ubiquitously expressed member of the forkhead box O family of winged helix transcription factors. In neurons, it is activated in response to stress, translocating to the nucleus where it promotes apoptosis and blocks proliferation. In insulin-responsive tissues, nutrient abundance triggers phosphorylation by AKT that blocks nuclear translocation and activity. A 60 kDa form, cleaved at R537, has been found in androgen-treated prostate cancer cells. Over aa 353‑655, human FoxO1/FKHR shares 93% aa identity with mouse and rat FoxO1/FKHR.

Long Name

Forkhead Box O1

Entrez Gene IDs

2308 (Human); 56458 (Mouse); 84482 (Rat)

Alternate Names

FKH1; FKHR; FKHRforkhead box protein O1; forkhead box O1; Forkhead box protein O1A; forkhead homolog in rhabdomyosarcoma; Forkhead in rhabdomyosarcoma; FoxO1; FOXO1Aforkhead, Drosophila, homolog of, in rhabdomyosarcoma