Human FoxO1/FKHR Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human FoxO1/FKHR in Western blots.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human FoxO1/FKHR
    Ala353-Gly655
    Accession # Q12778
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Simple Western
    10 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human FoxO1/FKHR by Western Blot. Western blot shows lysates of 293T human embryonic kidney cell line and JEG‑3 human epithelial choriocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human FoxO1/FKHR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5939) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FoxO1/FKHR at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human FoxO1/FKHR by Simple WesternTM. Simple Western lane view shows lysates of 293T human embryonic kidney cell line, loaded at 0.2 mg/mL. A specific band was detected for FoxO1/FKHR at approximately 93 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human FoxO1/FKHR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5939) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FoxO1/FKHR
FoxO1, also called FKHR (forkhead in ­rhabdomyosarcoma), is a 655 amino acid (aa), 70 kDa, ubiquitously expressed member of the forkhead box O family of winged helix transcription factors. In neurons, it is activated in response to stress, translocating to the nucleus where it promotes apoptosis and blocks proliferation. In insulin-responsive tissues, nutrient abundance triggers phosphorylation by AKT that blocks nuclear translocation and activity. A 60 kDa form, cleaved at R537, has been found in androgen-treated prostate cancer cells. Over aa 353‑655, human FoxO1/FKHR shares 93% aa identity with mouse and rat FoxO1/FKHR.
  • Long Name:
    Forkhead Box O1
  • Entrez Gene IDs:
    2308 (Human); 56458 (Mouse); 84482 (Rat)
  • Alternate Names:
    FKH1; FKHR; FKHRforkhead box protein O1; forkhead box O1; Forkhead box protein O1A; forkhead homolog in rhabdomyosarcoma; Forkhead in rhabdomyosarcoma; FoxO1; FOXO1Aforkhead, Drosophila, homolog of, in rhabdomyosarcoma
Related Research Areas
Isotype Controls
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Secondary Antibodies
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Sheep IgG (H+L) PE-conjugated Antibody

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Donkey Anti-Sheep IgG Biotinylated Antibody

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Sheep IgG (H+L) APC-conjugated Antibody

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Sheep IgG VisUCyte HRP Polymer Antibody

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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

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