Detection of Human Fucosyltransferase 11/FUT11 by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and NTera‑2 human testicular embryonic carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human Fucosyltransferase 11/FUT11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5964) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Fucosyltransferase 11/FUT11 at approximately 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Fucosyltransferase 11/FUT11 in NTera‑2 Human Cell Line.
Fucosyltransferase 11/FUT11 was detected in immersion fixed NTera‑2 human testicular embryonic carcinoma cell line using Human Fucosyltransferase 11/FUT11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5964) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Fucosyltransferase 11/FUT11
Fucose is frequently found at terminal sites on various glycans and is essential for the generation of many sugar epitopes. Well-known fucose containing glycans include Lewis and ABO blood group antigens. Lewis epitopes are key elements involved in leukocyte homing and extravasation process, thus are essential for lymphocyte maturation and natural defense functions (1). O-fucosylation on Notch receptor is found to be essential for its signaling function (2). So far, 11 carbohydrate specific fucosyltransferases are found in humans (3). FUT1 and FUT2 are alpha 1-2 fucosyltransferases and are responsible for ABO blood group antigen synthesis (4). FUT3, FUT4, FUT5, FUT6, FUT7, and FUT9 are alpha 1-3/4 fucosyltransferases and are responsible for Lewis antigen generation (5). FUT10 and FUT11 are newly cloned alpha 1-3 fucosyltransferases that are distinct from the alpha 1-3/4 fucosyltransferase subfamily and are able to introduce fucose to the innermost core GlcNAc of the N-glycan on conalbumin glycopeptides and biantennary N-glycan acceptors but not onto short lactosaminyl acceptor substrates (6). Predicted as a type II transmembrane protein and a Golgi resident enzyme, the exact function of this enzyme needs to be further characterized.
Weston, B.W. et al. (1992) J. Biol.Chem. 267:4152.
Stahl, M. et al. (2008) J. Biol.Chem. 283:13638.
Becker, D.J. et al. (2003) Glycobiology 13:41R.
Kelly, R.J. et al. (1995) J. Biol.Chem. 270:4640.
Weston, B.W. et al. (1992) J. Biol.Chem. 267:24575.
Mollicone, R. et al. (2009) J. Biol. Chem. 284:4723.
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