Human Fucosyltransferase 3/5 (FUT3/5) Antibody

Name: Human Fucosyltransferase 3/5 (FUT3/5) Antibody
Brand: R&D Systems
SKU: MAB4068-SP
Rating: 3.5 (2 reviews)

Catalog #: MAB4068 2 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4068
MAB4068-SP

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Reviews (2)

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Fucosyltransferase 3/5 (FUT3/5) Antibody Summary

Species Reactivity

Human

Specificity

Detects human Fucosyltransferase 3/5 (FUT3/5) in Western blots. The amino acid sequences of recombinant human FUT3 and recombinant human FUT5 are 91% identical.

Source

Monoclonal Mouse IgG₁ Clone # 425037

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Fucosyltransferase 3
Arg35-Thr361
Accession # P21217

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human Fucosyltransferase 3/FUT3 (Catalog # 4950-GT)
Recombinant Human Fucosyltransferase 5/FUT5 (Catalog # 4949-GT)

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human Fucosyltransferase 3/FUT3 (Catalog # 4950-GT), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fucosyltransferase 3/5 (FUT3/5)

Fucosyltransferase 3 (FUT3), also known as galactoside 3(4)-L-fucosyltransferase or blood group Lewis alpha -4-fucosyltransferase (Lewis FT), is type II membrane protein with a cytoplasmic tail (aa 1 to 15), a transmembrane region (aa 16 to 34) and an ectodomain (aa 35 to 361). The amino acid sequence of human FUT3 is 99% and 52% identical to that of chimpanzee and chicken.

Entrez Gene IDs

2525 (Human)

Alternate Names

CD174; FT3B; Fucosyltransferase 3/5; FucT-III; LE; Les