Human FYB/ADAP/SLAP130 Antibody Summary
Accession # O15117
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human FYB/ADAP/SLAP130 by Western Blot. Western blot shows nuclear extracts of Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 0.1 µg/mL of Human FYB/ADAP/SLAP130 Monoclonal Antibody (Catalog # MAB3857) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for FYB/ADAP/SLAP130 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Human SH2 domain containing leukocyte protein-76-Associated Phosphoprotein of 130 kDa (SLAP130), also known as Fyb-120 and ADAP, is an 86 kDa, 783 amino acid, cytoplasmic protein that runs anomalously as a 120‑130 kDa protein in SDS-PAGE. Human SLAP130 is a hematopoietic-specific adaptor that associates with other cytoplasmic proteins such as SH2-containing Leukocyte Phosphoprotein of 76 kDa (SLP-76). Adaptors integrate signals from surface receptors and are essential components of many immune cell-signaling cascades. There is at least one known alternatively spliced SLAP130 isoform which contains an additional 46 aa. Over the region used as immunogen, SLAP130 is 66% and 76% identical to the corresponding canine and mouse proteins, respectively.
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