Human FYB/ADAP/SLAP130 Antibody

Catalog # Availability Size / Price Qty
MAB3857
MAB3857-SP
Detection of Human FYB/ADAP/SLAP130 by Western Blot.
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Human FYB/ADAP/SLAP130 Antibody Summary

Species Reactivity
Human
Specificity
Detects human FYB/ADAP/SLAP130 in Western blots.
Source
Monoclonal Mouse IgG3 Clone # 460114
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human FYB/ADAP/SLAP130
Ala2-Gly274
Accession # O15117
Formulation
Lyophilized from a 0.2 μm filtered solution in Tris and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human FYB/ADAP/SLAP130 antibody by Western Blot. View Larger

Detection of Human FYB/ADAP/SLAP130 by Western Blot. Western blot shows nuclear extracts of Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 0.1 µg/mL of Human FYB/ADAP/SLAP130 Monoclonal Antibody (Catalog # MAB3857) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for FYB/ADAP/SLAP130 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FYB/ADAP/SLAP130

Human SH2 domain containing leukocyte protein-76-Associated Phosphoprotein of 130 kDa (SLAP130), also known as Fyb-120 and ADAP, is an 86 kDa, 783 amino acid, cytoplasmic protein that runs anomalously as a 120‑130 kDa protein in SDS-PAGE. Human SLAP130 is a hematopoietic-specific adaptor that associates with other cytoplasmic proteins such as SH2-containing Leukocyte Phosphoprotein of 76 kDa (SLP-76). Adaptors integrate signals from surface receptors and are essential components of many immune cell-signaling cascades. There is at least one known alternatively spliced SLAP130 isoform which contains an additional 46 aa. Over the region used as immunogen, SLAP130 is 66% and 76% identical to the corresponding canine and mouse proteins, respectively.

Long Name
FYN Binding Protein (FYB-120/130)
Entrez Gene IDs
2533 (Human); 23880 (Mouse); 499537 (Rat)
Alternate Names
ADAP; Adhesion and degranulation promoting adaptor protein; adhesion and degranulation-promoting adaptor protein; FYB; FYB-120/130SLAP-130FYN-binding protein (FYB-120/130); FYN binding protein; FYN-binding protein; FYN-T-binding protein; p120/p130; PRO0823; SLAP130; SLP-76-associated phosphoprotein

Product Datasheets

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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