Human GAB3 Antibody (720306)

R&D Systems | Catalog # MAB7127

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 720306
View all GAB3 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human GAB3
Leu121-Asn263
Accession # Q8WWW8

Specificity

Detects human GAB-3 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human GAB3 Antibody (720306)

Detection of Human GAB3 antibody by Western Blot.

Detection of Human GAB3 by Western Blot.

Western blot shows lysates of human spleen tissue and human thymus tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human GAB3 Monoclonal Antibody (Catalog # MAB7127) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for GAB3 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human GAB3 Antibody (720306)

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: Human spleen tissue and human thymus tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GAB3

GAB3 (GRB2-associated-binding protein 3) is a ~75-80 kDa member of the Gab family of scaffolding proteins which show overlapping expression in hematopoietic cells. All contain an N-terminal pleckstrin homology domain and multiple tyrosine phosphorylation and SH2 and SH3 domain binding sites.  M-CSF stimulation of myeloid precursors  through M-CSF R co-induces GAB3 and the associated adaptor protein GRAP2, and causes macrophage differentiation associated with tyrosine phosphorylation of GAB3. Over amino acids (aa) 121-263, human GAB3 shares 73% aa identity with mouse GAB3.  A potential isoform of 548 aa lacks aa 509-548, while another of 488 aa lacks aa 173-356 and diverges at aa 475. A third potential isoform of 206 aa has an alternate start site at aa 380.

Long Name

GRB2-associated Binding Protein 3

Alternate Names

DOS/Gab family member 3, Gab3 scaffolding protein, GRB2-associated binder 3, GRB2-associated binding protein 3, GRB2-associated-binding protein 3, Growth factor receptor bound protein 2-associated protein 3

Entrez Gene IDs

139716 (Human); 210710 (Mouse)

Gene Symbol

GAB3

UniProt

Q8WWW8

Additional GAB3 Products

Product Documents for Human GAB3 Antibody (720306)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human GAB3 Antibody (720306)

For research use only

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FAQs

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Associated Pathways

Akt Signaling Pathway
Akt Signaling Pathway Thumbnail