Human GAB3 Antibody Summary
Accession # Q8WWW8
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human GAB3 by Western Blot. Western blot shows lysates of human spleen tissue and human thymus tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human GAB3 Monoclonal Antibody (Catalog # MAB7127) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for GAB3 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
GAB3 (GRB2-associated-binding protein 3) is a ~75-80 kDa member of the Gab family of scaffolding proteins which show overlapping expression in hematopoietic cells. All contain an N-terminal pleckstrin homology domain and multiple tyrosine phosphorylation and SH2 and SH3 domain binding sites. M-CSF stimulation of myeloid precursors through M-CSF R co-induces GAB3 and the associated adaptor protein GRAP2, and causes macrophage differentiation associated with tyrosine phosphorylation of GAB3. Over amino acids (aa) 121-263, human GAB3 shares 73% aa identity with mouse GAB3. A potential isoform of 548 aa lacks aa 509-548, while another of 488 aa lacks aa 173-356 and diverges at aa 475. A third potential isoform of 206 aa has an alternate start site at aa 380.
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