Human GAB3 Antibody

Catalog # Availability Size / Price Qty
MAB7127
MAB7127-SP
Detection of Human GAB3 by Western Blot.
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Human GAB3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human GAB-3 in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG1 Clone # 720306
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human GAB3
Leu121-Asn263
Accession # Q8WWW8
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human GAB3 antibody by Western Blot. View Larger

Detection of Human GAB3 by Western Blot. Western blot shows lysates of human spleen tissue and human thymus tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human GAB3 Monoclonal Antibody (Catalog # MAB7127) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for GAB3 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GAB3

GAB3 (GRB2-associated-binding protein 3) is a ~75-80 kDa member of the Gab family of scaffolding proteins which show overlapping expression in hematopoietic cells. All contain an N-terminal pleckstrin homology domain and multiple tyrosine phosphorylation and SH2 and SH3 domain binding sites.  M-CSF stimulation of myeloid precursors  through M-CSF R co-induces GAB3 and the associated adaptor protein GRAP2, and causes macrophage differentiation associated with tyrosine phosphorylation of GAB3. Over amino acids (aa) 121-263, human GAB3 shares 73% aa identity with mouse GAB3.  A potential isoform of 548 aa lacks aa 509-548, while another of 488 aa lacks aa 173-356 and diverges at aa 475. A third potential isoform of 206 aa has an alternate start site at aa 380.

Long Name
GRB2-associated Binding Protein 3
Entrez Gene IDs
139716 (Human); 210710 (Mouse)
Alternate Names
DOS/Gab family member 3; Gab3 scaffolding protein; GAB3; GRB2-associated binder 3; GRB2-associated binding protein 3; GRB2-associated-binding protein 3; Growth factor receptor bound protein 2-associated protein 3

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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