Human Galectin-3 Quantikine ELISA Kit

Catalog # Availability Size / Price Qty
DGAL30
SGAL30
PDGAL30
Control Products Available
Human Galectin-3 ELISA/LGALS3 ELISA Cell Culture Supernate Standard Curve
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Product Details
Procedure
Citations (31)
FAQs
Supplemental Products
Reviews (7)

Human Galectin-3 Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
Sensitivity
0.085 ng/mL
Assay Range
0.3 - 10 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human Galectin-3.  This assay cross-reacts 58% with Mouse Galectin-3.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity observed with 1 or more species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human Galectin-3 Immunoassay is a 4.5 hour solid phase ELISA designed to measure Galectin-3 levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human Galectin-3 and antibodies raised against the recombinant protein. Results obtained for naturally occurring human Galectin-3 showed linear curves that were parallel to the standard curves obtained using the kit standards. These results indicate that this kit can be used to determine relative mass values for natural human Galectin-3.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (ng/mL) 0.78 2.27 5.06 0.81 2.51 5.29
Standard Deviation 0.03 0.1 0.15 0.07 0.2 0.36
CV% 3.8 4.4 3 8.6 8 6.8

Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (ng/mL) 0.79 2.46 5.11 0.8 2.59 5.5
Standard Deviation 0.03 0.09 0.18 0.05 0.16 0.32
CV% 3.8 3.7 3.5 6.3 6.2 5.8

Recovery

The recovery of Galectin-3 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 102 96-106
EDTA Plasma (n=4) 105 93-113
Heparin Plasma (n=4) 103 95-110
Serum (n=4) 104 94-115

Linearity

To assess the linearity of the assay, samples containing or spiked with high concentrations of human Galectin-3 in various matrices were diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human Galectin-3 ELISA/LGALS3 ELISA Linearity

Scientific Data

Human Galectin-3 ELISA/LGALS3 ELISA Cell Culture Supernate Standard Curve

Human Galectin-3 ELISA/LGALS3 ELISA Serum/Plasma Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Galectin-3

The galectins constitute a large family of carbohydrate-binding proteins that function in many systems both intracellularly and following secretion. Galectins contain either one or two carbohydrate recognition domains (CRR) which mediate recognition of N-acetyl-lactosamine-containing glycoproteins. Some galectins exist in multiple isoforms due to alternative splicing. Individual galectins differ in their tissue distribution and in their carbohydrate-binding specificities.

Entrez Gene IDs:
3958 (Human); 16854 (Mouse)
Alternate Names:
AGE-R3; Carbohydrate-binding protein 35; CBP 35,35 kDa lectin; CBP35; GAL3; Gal-3; galactin-3; Galactose-specific lectin 3; Galactoside-binding protein; GALBPCBP35; Galectin3; Galectin-3; GALIG; IgE-binding protein; L29; L31; L-31; Laminin-binding protein; Lectin L-29; lectin, galactoside-binding, soluble, 3; LGALS2; LGALS3; Mac-2 antigen; Mac-2; MAC2GAL3
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  10.   Aspirate and wash 4 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human Galectin-3 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

31 Citations: Showing 1 - 10
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  1. Plasma galectin-3 concentration and estimated glomerular filtration rate in patients with type 2 diabetes with and without albuminuria
    Authors: JO Chung, SY Park, SB Lee, NR Kang, DH Cho, DJ Chung, MY Chung
    Scientific Reports, 2022;12(1):16328.
    Species: Human
    Sample Types: Plasma
  2. The interaction between rs 3,807,992 genotypes with the dietary inflammatory index on Leptin, Leptin resistance, and Galectin 3 in obese and overweight women
    Authors: F Shiraseb, M Farazi, N Rasaei, CCT Clark, S Jamili, K Mirzaei
    BMC endocrine disorders, 2022;22(1):237.
    Species: Human
    Sample Types: Serum
  3. Potential Protective Role of Galectin-3 in Patients with Gonarthrosis and Diabetes Mellitus: A Cross-Sectional Study
    Authors: J Milosevic, M Jurisevic, V Grbovic, I Jovanovic, N Gajovic, A Jurisic-Sk
    International Journal of Environmental Research and Public Health, 2022;19(18):.
    Species: Human
    Sample Types: Serum
  4. Therapeutic implications of galectin-3 in patients with atrial fibrillation
    Authors: KN Lee, DY Kim, KY Boo, YG Kim, SY Roh, YS Baek, DH Kim, DI Lee, J Shim, JI Choi, GS Hwang, YH Kim
    Scientific Reports, 2022;12(1):784.
    Species: Human
    Sample Types: Serum
  5. Serum Galectin-3 levels and all-cause and cardiovascular mortality in maintenance hemodialysis patients: a prospective cohort study
    Authors: S Liu, Q Wu, S Zhang, Z Wang, H Liu, L Teng, P Xiao, Y Lu, X Wang, C Dong, J Xiao, J Zhang
    Bmc Nephrology, 2022;23(1):5.
    Species: Human
    Sample Types: Serum
  6. The value of plasma hypoxia markers for predicting imaging-based hypoxia in patients with head-and-neck cancers undergoing definitive chemoradiation
    Authors: A Rühle, AL Grosu, N Wiedenmann, J Ruf, B Bieber, R Stoian, AR Thomsen, E Gkika, P Vaupel, D Baltas, WA Weber, M Mix, NH Nicolay
    Clinical and translational radiation oncology, 2022;33(0):120-127.
    Species: Human
    Sample Types: Plasma
  7. Effects of liraglutide vs. lifestyle changes on soluble suppression of tumorigenesis-2 (sST2) and galectin-3 in obese subjects with prediabetes or type 2 diabetes after comparable weight loss
    Authors: P Simeone, R Tripaldi, A Michelsen, T Ueland, R Liani, S Ciotti, KI Birkeland, HL Gulseth, A Di Casteln, F Cipollone, P Aukrust, A Consoli, B Halvorsen, F Santilli
    Cardiovascular Diabetology, 2022;21(1):36.
    Species: Human
    Sample Types: Serum
  8. Adaptive response triggered by the repeated SCUBA diving is reflected in cardiovascular, muscular, and immune biomarkers
    Authors: M Žarak, A Perovi?, M Njire Brat, S Šupraha Go, J Dumi?
    Physiological Reports, 2021;9(2):e14691.
    Species: Human
    Sample Types: Plasma
  9. Myocardial extracellular volume quantification by computed tomography predicts outcomes in patients with severe aortic stenosis
    Authors: Y Hammer, Y Talmor-Bar, A Abelow, K Orvin, Y Aviv, N Bar, A Levi, U Landes, G Shafir, A Barsheshet, H Vaknin-Ass, A Sagie, R Kornowski, A Hamdan
    PLoS ONE, 2021;16(3):e0248306.
    Species: Human
    Sample Types: Serum
  10. Analysis of the Correlation of Galectin-3 Concentration with the Measurements of Echocardiographic Parameters Assessing Left Atrial Remodeling and Function in Patients with Persistent Atrial Fibrillation
    Authors: P Wa?ek, U Grabowska, E Cie?la, J Sielski, J Roskal-Wa?, B Wo?akowska
    Biomolecules, 2021;11(8):.
    Species: Human
    Sample Types: Serum
  11. Diagnostic Significance of Serum Galectin-3 in Hospitalized Patients with COVID-19-A Preliminary Study
    Authors: B Ku?nierz-C, B Maziarz, P Dumnicka, M Dembi?ski, M Kapusta, M Boci?ga-Ja, M Winiarski, A Garlicki, T Grodzicki, M Kukla
    Oncogene, 2021;11(8):.
    Species: Human
    Sample Types: Serum
  12. The immunoregulatory function of peripheral blood CD71+ erythroid cells in systemic-onset juvenile idiopathic arthritis
    Authors: H Kanemasa, M Ishimura, K Eguchi, T Tanaka, E Nanishi, A Shiraishi, M Goto, Y Motomura, S Ohga
    Scientific Reports, 2021;11(1):14396.
    Species: Human
    Sample Types: Serum
  13. Galactin-1, 3 and 9: Potential biomarkers in idiopathic pulmonary fibrosis and other interstitial lung diseases
    Authors: M d'Alessand, E De Vita, L Bergantini, MA Mazzei, S di Valvaso, M Bonizzoli, A Peris, P Sestini, E Bargagli, D Bennett
    Respir Physiol Neurobiol, 2020;282(0):103546.
    Species: Human
    Sample Types: Serum
  14. Systemic right ventricle in elderly patients with congenitally corrected transposition of the great arteries: Clinical profile, cardiac biomarkers, and echocardiographic parameters
    Authors: E Kowalik, A Kwiatek-Wr, A Klisiewicz, A Lutynska, EK Biernacka, M Kowalski, P Hoffman
    Anatol J Cardiol, 2020;24(2):92-96.
    Species: Human
    Sample Types: Plasma
  15. Galectin-3 enhances neutrophil motility and extravasation into the airways during Aspergillus fumigatus�infection
    Authors: BD Snarr, G St-Pierre, B Ralph, M Lehoux, Y Sato, A Rancourt, T Takazono, SR Baistrocch, R Corsini, MP Cheng, M Sugrue, LR Baden, K Izumikawa, H Mukae, JR Wingard, IL King, M Divangahi, MS Satoh, BG Yipp, S Sato, DC Sheppard
    PLoS Pathog., 2020;16(8):e1008741.
    Species: Human
    Sample Types: Serum
  16. Proteomic identification of early urinary-biomarkers of acute kidney injury in preterm infants
    Authors: YH Jung, D Han, SH Shin, EK Kim, HS Kim
    Sci Rep, 2020;10(1):4057.
    Species: Human
    Sample Types: Urine
  17. Evaluation of Inflammation Caused by Cardiopulmonary Bypass in a Small Animal Model
    Authors: Y Fujii
    Biology (Basel), 2020;9(4):.
    Species: Rat
    Sample Types: Plasma
  18. Systemic Inflammation Precedes Microalbuminuria in Diabetes
    Authors: FG Scurt, J Menne, S Brandt, A Bernhardt, PR Mertens, H Haller, C Chatzikyrk,
    Kidney Int Rep, 2019;4(10):1373-1386.
    Species: Human
    Sample Types: Serum
  19. The prognostic value of sST2 and galectin-3 considering different aetiologies in non-ischaemic heart failure
    Authors: D Binas, H Daniel, A Richter, V Ruppert, KD Schlüter, B Schieffer, S Pankuweit
    Open Heart, 2018;5(1):e000750.
    Species: Human
    Sample Types: Serum
  20. Evaluation of the association between serum uric acid level andthe predicted risk score of sudden cardiac death in five years in patients with hypertrophic cardiomyopathy
    Authors: S Özy?lmaz, MH Sat?lm??o?, M Gül, H Uyarel, OA Serdar
    Turk Kardiyol Dern Ars, 2018;46(2):111-120.
    Species: Human
    Sample Types: Serum
  21. Combination of Plasma Biomarkers and Clinical Data for the Detection of Myocardial Fibrosis or Aggravation of Heart Failure Symptoms in Heart Failure with Preserved Ejection Fraction Patients
    Authors: CK Wu, MM Su, YF Wu, JJ Hwang, LY Lin
    J Clin Med, 2018;7(11):.
    Species: Human
    Sample Types: Plasma
  22. Elevated Inflammatory Plasma Biomarkers in Patients With Fabry Disease: A Critical Link to Heart Failure With Preserved Ejection Fraction
    Authors: H Yogasundar, A Nikhanj, BN Putko, M Boutin, S Jain-Ghai, A Khan, C Auray-Blai, ML West, GY Oudit
    J Am Heart Assoc, 2018;7(21):e009098.
    Species: Human
    Sample Types: Plasma
  23. Prognostic Utility of Soluble Suppression of Tumorigenicity 2 level as a Predictor of Clinical Outcomes in Incident Hemodialysis Patients
    Authors: SM Seo, SH Kim, Y Kim, HE Yoon, SJ Shin
    Int J Med Sci, 2018;15(7):730-737.
    Species: Human
    Sample Types: Plasma
  24. Work Intensity, Low-Grade Inflammation, and Oxidative Status: A Comparison between Office and Slaughterhouse Workers
    Authors: S Zelzer, F Tatzber, M Herrmann, W Wonisch, S Rinnerhofe, M Kundi, B Obermayer-, T Niedrist, G Cvirn, G Wultsch, H Mangge
    Oxid Med Cell Longev, 2018;2018(0):2737563.
    Species: Human
    Sample Types: Serum
  25. Galectin-3 and incident cognitive impairment in REGARDS, a cohort of blacks and whites
    Authors: A Venkatrama, P Callas, LA McClure, F Unverzagt, G Arora, V Howard, VG Wadley, M Cushman, P Arora
    Alzheimers Dement (N Y), 2018;4(0):165-172.
    Species: Human
    Sample Types: Serum
  26. Serum Galectin-3 and ST2 as predictors of unfavorable outcome in stable dilated cardiomyopathy patients
    Authors: C Wojciechow, E Romuk, E Nowalany-K, W Jache?
    Hellenic J Cardiol, 2017;0(0):.
    Species: Human
    Sample Types: Serum
  27. High Expression of Galectin-3 in Patients with IgG4-Related Disease: A Proteomic Approach
    Authors: A Salah, H Yoshifuji, S Ito, K Kitagori, K Kiso, N Yamada, T Nakajima, H Haga, T Tsuruyama, A Miyagawa-H
    Patholog Res Int, 2017;2017(0):9312142.
    Species: Human
    Sample Types: Serum
  28. Serum levels of galectin-1, galectin-3, and galectin-9 are associated with large artery atherosclerotic stroke
    Authors: XW He, WL Li, C Li, P Liu, YG Shen, M Zhu, XP Jin
    Sci Rep, 2017;7(0):40994.
    Species: Human
    Sample Types: Serum
  29. Aldosterone induced galectin-3 secretion in vitro and in vivo: from cells to humans.
    Authors: Lin Y, Chou C, Wu X, Chang Y, Hung C, Chen Y, Tzeng Y, Wu V, Ho Y, Hsieh F, Wu K
    PLoS ONE, 2014;9(9):e95254.
  30. Galectin-3 in cord blood of term and preterm infants.
    Authors: Demmert M, Faust K, Bohlmann MK, Troger B, Gopel W, Herting E, Hartel C
    Clin. Exp. Immunol., 2012;167(2):246-51.
    Species: Human
    Sample Types: Cell Culture Supernates
  31. Hypoxia potentiates glioma-mediated immunosuppression.
    Authors: Wei J, Wu A, Kong LY, Wang Y, Fuller G, Fokt I, Melillo G, Priebe W, Heimberger AB
    PLoS ONE, 2012;6(1):e16195.
    Species: Human
    Sample Types: Cell Culture Supernates

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Reviews for Human Galectin-3 Quantikine ELISA Kit

Average Rating: 4.9 (Based on 7 Reviews)

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Human Galectin-3 Quantikine ELISA Kit
By Anonymous on 09/26/2022
Sample Tested: Serum

Human Galectin-3 Quantikine ELISA Kit
By Anonymous on 09/20/2022
Sample Tested: Serum

Human Galectin-3 Quantikine ELISA Kit
By Anonymous on 05/26/2022
Sample Tested: Serum

Human Galectin-3 Quantikine ELISA Kit
By Anonymous on 01/25/2022
Sample Tested: Serum and Plasma

Human Galectin-3 Quantikine ELISA Kit
By Shinsuke Hoshino on 10/15/2018
Sample Tested: Plasma

It was very useful and easy to understand to use.
The support was excellent, thank you.


Human Galectin-3 Quantikine ELISA Kit
By Anonymous on 10/08/2018
Sample Tested: HASMC human aortic smooth muscle cells

Human Galectin-3 Quantikine ELISA Kit
By Anonymous on 08/29/2018
Sample Tested: Serum