Human Galectin-3 Quantikine ELISA Kit

Catalog # Availability Size / Price Qty
DGAL30
SGAL30
PDGAL30
Control Products Available
Human Galectin-3 ELISA Cell Culture Supernate Standard Curve
3 Images
Product Details
Procedure
Citations (9)
FAQs
Supplemental Products
Reviews (1)

Human Galectin-3 Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
Sensitivity
0.085 ng/mL
Assay Range
0.3 - 10 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human Galectin-3.  This assay cross-reacts 58% with Mouse Galectin-3.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity observed with 1 or more species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human Galectin-3 Immunoassay is a 4.5 hour solid phase ELISA designed to measure Galectin-3 levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human Galectin-3 and antibodies raised against the recombinant protein. Results obtained for naturally occurring human Galectin-3 showed linear curves that were parallel to the standard curves obtained using the kit standards. These results indicate that this kit can be used to determine relative mass values for natural human Galectin-3.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 0.78 2.27 5.06 0.81 2.51 5.29
Standard Deviation 0.03 0.1 0.15 0.07 0.2 0.36
CV% 3.8 4.4 3 8.6 8 6.8

Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 0.79 2.46 5.11 0.8 2.59 5.5
Standard Deviation 0.03 0.09 0.18 0.05 0.16 0.32
CV% 3.8 3.7 3.5 6.3 6.2 5.8

Recovery

The recovery of Galectin-3 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 102 96-106
EDTA Plasma (n=4) 105 93-113
Heparin Plasma (n=4) 103 95-110
Serum (n=4) 104 94-115

Linearity

To assess the linearity of the assay, samples containing or spiked with high concentrations of human Galectin-3 in various matrices were diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human Galectin-3 ELISA Linearity

Data Examples

Human Galectin-3 ELISA Cell Culture Supernate Standard Curve

Human Galectin-3 ELISA Serum/Plasma Standard Curve

Product Datasheets

Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Galectin-3

The galectins constitute a large family of carbohydrate-binding proteins that function in many systems both intracellularly and following secretion. Galectins contain either one or two carbohydrate recognition domains (CRR) which mediate recognition of N-acetyl-lactosamine-containing glycoproteins. Some galectins exist in multiple isoforms due to alternative splicing. Individual galectins differ in their tissue distribution and in their carbohydrate-binding specificities.

Entrez Gene IDs:
3958 (Human); 16854 (Mouse)
Alternate Names:
AGE-R3; Carbohydrate-binding protein 35; CBP 35,35 kDa lectin; CBP35; GAL3; Gal-3; galactin-3; Galactose-specific lectin 3; Galactoside-binding protein; GALBPCBP35; Galectin3; Galectin-3; GALIG; IgE-binding protein; L29; L31; L-31; Laminin-binding protein; Lectin L-29; lectin, galactoside-binding, soluble, 3; LGALS2; LGALS3; Mac-2 antigen; Mac-2; MAC2GAL3
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  10.   Aspirate and wash 4 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human Galectin-3 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Galectin-3 and incident cognitive impairment in REGARDS, a cohort of blacks and whites
    Authors: A Venkatrama, P Callas, LA McClure, F Unverzagt, G Arora, V Howard, VG Wadley, M Cushman, P Arora
    Alzheimers Dement (N Y), 2018;4(0):165-172.
    Species: Human
    Sample Types: Serum
  2. Work Intensity, Low-Grade Inflammation, and Oxidative Status: A Comparison between Office and Slaughterhouse Workers
    Authors: S Zelzer, F Tatzber, M Herrmann, W Wonisch, S Rinnerhofe, M Kundi, B Obermayer-, T Niedrist, G Cvirn, G Wultsch, H Mangge
    Oxid Med Cell Longev, 2018;2018(0):2737563.
    Species: Human
    Sample Types: Serum
  3. Prognostic Utility of Soluble Suppression of Tumorigenicity 2 level as a Predictor of Clinical Outcomes in Incident Hemodialysis Patients
    Authors: SM Seo, SH Kim, Y Kim, HE Yoon, SJ Shin
    Int J Med Sci, 2018;15(7):730-737.
    Species: Human
    Sample Types: Plasma
  4. Serum levels of galectin-1, galectin-3, and galectin-9 are associated with large artery atherosclerotic stroke
    Authors: XW He, WL Li, C Li, P Liu, YG Shen, M Zhu, XP Jin
    Sci Rep, 2017;7(0):40994.
    Species: Human
    Sample Types: Serum
  5. High Expression of Galectin-3 in Patients with IgG4-Related Disease: A Proteomic Approach
    Authors: A Salah, H Yoshifuji, S Ito, K Kitagori, K Kiso, N Yamada, T Nakajima, H Haga, T Tsuruyama, A Miyagawa-H
    Patholog Res Int, 2017;2017(0):9312142.
    Species: Human
    Sample Types: Serum
  6. Serum Galectin-3 and ST2 as predictors of unfavorable outcome in stable dilated cardiomyopathy patients
    Authors: C Wojciechow, E Romuk, E Nowalany-K, W Jache?
    Hellenic J Cardiol, 2017;0(0):.
    Species: Human
    Sample Types: Serum
  7. Aldosterone induced galectin-3 secretion in vitro and in vivo: from cells to humans.
    Authors: Lin Y, Chou C, Wu X, Chang Y, Hung C, Chen Y, Tzeng Y, Wu V, Ho Y, Hsieh F, Wu K
    PLoS ONE, 2014;9(9):e95254.
  8. Hypoxia potentiates glioma-mediated immunosuppression.
    Authors: Wei J, Wu A, Kong LY, Wang Y, Fuller G, Fokt I, Melillo G, Priebe W, Heimberger AB
    PLoS ONE, 2012;6(1):e16195.
    Species: Human
    Sample Types: Cell Culture Supernates
  9. Galectin-3 in cord blood of term and preterm infants.
    Authors: Demmert M, Faust K, Bohlmann MK, Troger B, Gopel W, Herting E, Hartel C
    Clin. Exp. Immunol., 2012;167(2):246-51.
    Species: Human
    Sample Types: Cell Culture Supernates

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Human Galectin-3 Quantikine ELISA Kit
By Anonymous on 08/29/2018
Sample Tested: Serum