Human Gastrokine 1 Antibody

Catalog # Availability Size / Price Qty
AF6395
AF6395-SP
Human Gastrokine 1 Antibody in Western Blot
2 Images
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews

Human Gastrokine 1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Gastrokine 1 in direct ELISAs and Western blots. In direct ELISAs, less than 3% cross-reactivity with recombinant mouse Gastrokine 1 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Gastrokine 1
Asn35-Asn199
Accession # Q9NS71
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human Gastrokine 1 by Western Blot. View Larger

Detection of Human Gastrokine 1 by Western Blot. Western blot shows lysates of human stomach tissue. PVDF Membrane was probed with 1 µg/mL of Human Gastrokine 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6395) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Gastrokine 1 at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunohistochemistry Gastrokine 1 in Human Stomach. View Larger

Gastrokine 1 in Human Stomach. Gastrokine 1 was detected in immersion fixed paraffin-embedded sections of human stomach using Sheep Anti-Human Gastrokine 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6395) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to luminal part of glandular epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Gastrokine 1

Gastrokine 1 (GKN1; also CA11 and AMP-18) is an 18 kDa member of the CA11 protein family. It has limited expression, being restricted to mucous secreting pyloric atrial epithelium. Gastrokine 1 appears to promote epithelial proliferation and migration, and induce the formation of tight junctions between epithelial cells. Mature human Gastrokine 1 is 165 amino acids (aa) in length. Based on the SwissProt sequence, it possesses one BRICHOS domain (aa 54‑150) that contains a mitogenic sequence (aa 97‑117). There is one potential alternative start site 14 aa upstream of the standard site that does not appear to be utilized. Over aa 21‑185 (aa 35‑199 of SwissProt Q9NS71), human Gastrokine 1 shares 65% aa identity with mouse Gastrokine 1.

Long Name
Take out Column D name
Entrez Gene IDs
56287 (Human); 66283 (Mouse); 297418 (Rat)
Alternate Names
18 kDa antrum mucosa protein; AMP18; AMP-18; AMP18BRICHOS domain containing 1; BRICD1; CA11; CA11MGC70354; FOV; Foveolin; Gastrokine 1; gastrokine-1; GKN1; Protein CA11

Product Datasheets

Citation for Human Gastrokine 1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Keratin 14-high subpopulation mediates lung cancer metastasis potentially through Gkn1 upregulation
    Authors: S Yao, HY Huang, X Han, Y Ye, Z Qin, G Zhao, F Li, G Hu, L Hu, H Ji
    Oncogene, 2019;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human Gastrokine 1 Antibody

There are currently no reviews for this product. Be the first to review Human Gastrokine 1 Antibody and earn rewards!

Have you used Human Gastrokine 1 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review