Detection of Human Gastrokine 1 by Western Blot.
Western blot shows lysates of human stomach tissue. PVDF Membrane was probed with 1 µg/mL of Human Gastrokine 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6395) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Gastrokine 1 at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Gastrokine 1 in Human Stomach.
Gastrokine 1 was detected in immersion fixed paraffin-embedded sections of human stomach using Sheep Anti-Human Gastrokine 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6395) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to luminal part of glandular epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Gastrokine 1
Gastrokine 1 (GKN1; also CA11 and AMP-18) is an 18 kDa member of the CA11 protein family. It has limited expression, being restricted to mucous secreting pyloric atrial epithelium. Gastrokine 1 appears to promote epithelial proliferation and migration, and induce the formation of tight junctions between epithelial cells. Mature human Gastrokine 1 is 165 amino acids (aa) in length. Based on the SwissProt sequence, it possesses one BRICHOS domain (aa 54‑150) that contains a mitogenic sequence (aa 97‑117). There is one potential alternative start site 14 aa upstream of the standard site that does not appear to be utilized. Over aa 21‑185 (aa 35‑199 of SwissProt Q9NS71), human Gastrokine 1 shares 65% aa identity with mouse Gastrokine 1.
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