GATA‑3 in MCF-7 Human Cell Line.
GATA‑3 was detected in immersion fixed MCF-7 human breast cancer cell line using 10 µg/mL Goat Anti-Human GATA‑3 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2605) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Streptavidin (red, upper panel; Catalog # NL999) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
GATA-3 belongs to the GATA family of transcription factors, which bind
to the consensus DNA sequence (A/T) GATA (A/G) to control diverse
tissue-specific programs of gene expression and morphogenesis. It is
widely expressed in mesodermal- and endodermal-derived tissues. GATA-3
has been shown to be an essential regulator for immune cell function,
sympathetic neuron development and the maintenance of the
differentiated state in epithelial cells.
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