Human GATA-3 Antibody
Human GATA-3 Antibody Summary
Pro135-Ser258
Accession # P23771
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human and Mouse GATA‑3 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, Jurkat human acute T cell leukemia cell line, and EL-4 mouse lymphoblast cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human GATA-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2605) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for GATA-3 full length (FL) at approximately 55 kDa and the splice form (SF) at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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GATA‑3 in Human Breast Cancer Tissue. GATA-3 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Goat Anti-Human GATA-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2605) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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GATA‑3 in MCF-7 Human Cell Line. GATA-3 was detected in immersion fixed MCF-7 human breast cancer cell line using 10 µg/mL Goat Anti-Human GATA-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2605) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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GATA‑3 in Human Breast Cancer Tissue. GATA-3 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Goat Anti-Human GATA-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2605) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Detection of Human GATA‑3 by Simple WesternTM. Simple Western lane view shows lysates of MCF-7 human breast cancer cell line and Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. Specific bands were detected for GATA-3 full length (FL) at approximately 62 kDa and the splice form (SF) at approximately 52 kDa (as indicated) using 2.5 µg/mL of Goat Anti-Human GATA-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2605) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GATA-3
GATA-3 belongs to the GATA family of transcription factors, which bind to the consensus DNA sequence (A/T) GATA (A/G) to control diverse tissue-specific programs of gene expression and morphogenesis. It is widely expressed in mesodermal- and endodermal-derived tissues. GATA-3 has been shown to be an essential regulator for immune cell function, sympathetic neuron development and the maintenance of the differentiated state in epithelial cells.
Product Datasheets
Citations for Human GATA-3 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Organoid-on-a-chip model of human ARPKD reveals mechanosensing pathomechanisms for drug discovery
Authors: K Hiratsuka, T Miyoshi, KT Kroll, NR Gupta, MT Valerius, T Ferrante, M Yamashita, JA Lewis, R Morizane
Science Advances, 2022;8(38):eabq0866.
Species: Human
Sample Types: Organoids
Applications: IHC -
Delamination of trophoblast-like syncytia from the amniotic ectodermal analogue in human primed embryonic stem cell-based differentiation model
Authors: M Ohgushi, N Taniyama, A Vandenbon, M Eiraku
Cell Reports, 2022;39(12):110973.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Integrated pseudotime analysis of human pre-implantation embryo single-cell transcriptomes reveals the dynamics of lineage specification
Authors: D Meisterman, A Bruneau, S Loubersac, A Reignier, J Firmin, V François-C, S Kilens, Y Lelièvre, J Lammers, M Feyeux, P Hulin, S Nedellec, B Bretin, G Castel, N Allègre, S Covin, A Bihouée, M Soumillon, T Mikkelsen, P Barrière, C Chazaud, J Chappell, V Pasque, J Bourdon, T Fréour, L David
Cell Stem Cell, 2021;0(0):.
Species: Human
Sample Types: Embryo
Applications: IHC -
Gut Microbiota Modulate CD8�T Cell Responses to Influence Colitis-Associated Tumorigenesis
Authors: AI Yu, L Zhao, KA Eaton, S Ho, J Chen, S Poe, J Becker, A Gonzalez, D McKinstry, M Hasso, J Mendoza-Ca, J Whitfield, C Koumpouras, PD Schloss, EC Martens, GY Chen
Cell Rep, 2020;31(1):107471.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Developmental potential of aneuploid human embryos cultured beyond implantation
Authors: MN Shahbazi, T Wang, X Tao, BAT Weatherbee, L Sun, Y Zhan, L Keller, GD Smith, A Pellicer, RT Scott, E Seli, M Zernicka-G
Nat Commun, 2020;11(1):3987.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Mouse Embryonic Stem Cell-Derived Ureteric Bud Progenitors Induce Nephrogenesis
Authors: Z Tan, A Rak-Raszew, I Skovorodki, SJ Vainio
Cells, 2020;9(2):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Human Pluripotent Stem Cell-Derived Kidney Organoids with Improved Collecting Duct Maturation and Injury Modeling
Authors: K Uchimura, H Wu, Y Yoshimura, BD Humphreys
Cell Reports, 2020;33(11):108514.
Species: Human
Sample Types: Whole Cells
Applications: IHC -
Plasticity of distal nephron epithelia from human kidney organoids enables the induction of ureteric tip and stalk
Authors: SE Howden, SB Wilson, E Groenewege, L Starks, TA Forbes, KS Tan, JM Vanslambro, EM Holloway, YH Chen, S Jain, JR Spence, MH Little
Cell Stem Cell, 2020;0(0):.
Species: Transgenic Human
Sample Types: Organoid
Applications: IHC -
Developmental potential of aneuploid human embryos cultured beyond implantation
Authors: MN Shahbazi, T Wang, X Tao, BAT Weatherbee, L Sun, Y Zhan, L Keller, GD Smith, A Pellicer, RT Scott, E Seli, M Zernicka-G
Nat Commun, 2020;11(1):3987.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Pyrrothiogatain acts as an inhibitor of GATA family proteins and inhibits Th2 cell differentiation in vitro
Authors: S Nomura, H Takahashi, J Suzuki, M Kuwahara, M Yamashita, T Sawasaki
Sci Rep, 2019;9(1):17335.
Species: Mouse
Sample Types: Cell Lysates
Applications: ChIP -
Tubuloids derived from human adult kidney and urine for personalized disease modeling
Authors: F Schutgens, MB Rookmaaker, T Margaritis, A Rios, C Ammerlaan, J Jansen, L Gijzen, M Vormann, A Vonk, M Viveen, FY Yengej, S Derakhshan, KM de Winter-, B Artegiani, R van Boxtel, E Cuppen, APA Hendrickx, MM van den He, E Heitzer, H Lanz, J Beekman, JL Murk, R Masereeuw, F Holstege, J Drost, MC Verhaar, H Clevers
Nat. Biotechnol., 2019;37(3):303-313.
Species: Human
Sample Types: Organoids
Applications: IHC -
Evaluation of variability in human kidney organoids
Authors: B Phipson, PX Er, AN Combes, TA Forbes, SE Howden, L Zappia, HJ Yen, KT Lawlor, LJ Hale, J Sun, E Wolvetang, M Takasato, A Oshlack, MH Little
Nat. Methods, 2019;16(1):79-87.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Trophoblast organoids as a model for maternal-fetal interactions during human placentation
Authors: MY Turco, L Gardner, RG Kay, RS Hamilton, M Prater, MS Hollinshea, A McWhinnie, L Esposito, R Fernando, H Skelton, F Reimann, FM Gribble, A Sharkey, SGE Marsh, S O'Rahilly, M Hemberger, GJ Burton, A Moffett
Nature, 2018;0(0):.
Species: Human
Sample Types: Trophoblast Organoids
Applications: IHC -
GATA3 targets semaphorin 3B in mammary epithelial cells to suppress breast cancer progression and metastasis
Authors: P Shahi, CY Wang, J Chou, C Hagerling, H Gonzalez V, A Ruderisch, Y Yu, MD Lai, Z Werb
Oncogene, 2017;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Kidney organoids from human iPS cells contain multiple lineages and model human nephrogenesis.
Authors: Takasato M, Er P, Chiu H, Maier B, Baillie G, Ferguson C, Parton R, Wolvetang E, Roost M, Chuva de Sousa Lopes S, Little M
Nature, 2015;526(7574):564-8.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Human cytomegalovirus infection interferes with the maintenance and differentiation of trophoblast progenitor cells of the human placenta.
Authors: Tabata T, Petitt M, Zydek M, Fang-Hoover J, Larocque N, Tsuge M, Gormley M, Kauvar L, Pereira L
J Virol, 2015;89(9):5134-47.
Species: Human
Sample Types: Whole Cells
Applications: IHC-P -
A Gata3-Mafb transcriptional network directs post-synaptic differentiation in synapses specialized for hearing.
Authors: Yu, Wei-Ming, Appler, Jessica, Kim, Ye-Hyun, Nishitani, Allison, Holt, Jeffrey, Goodrich, Lisa V
Elife, 2013;2(0):e01341.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Cytokines inhibit norepinephrine transporter expression by decreasing Hand2.
Authors: Pellegrino MJ, Parrish DC, Zigmond RE, Habecker BA
Mol. Cell. Neurosci., 2011;46(3):671-80.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Critical role for GATA3 in mediating Tie2 expression and function in large vessel endothelial cells.
Authors: Song H, Suehiro J, Kanki Y, Kawai Y, Inoue K, Daida H, Yano K, Ohhashi T, Oettgen P, Aird WC, Kodama T, Minami T
J. Biol. Chem., 2009;284(42):29109-24.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Novel immunomodulatory properties of berbamine through selective down-regulation of STAT4 and action of IFN-gamma in experimental autoimmune encephalomyelitis.
Authors: Ren Y, Lu L, Guo TB, Qiu J, Yang Y, Liu A, Zhang JZ
J. Immunol., 2008;181(2):1491-8.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
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use at 10 µg/ml
