Human GCHFR Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human GCHFR in ELISAs.
  • Source
    Monoclonal Mouse IgG1 Clone # 846011
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human GCHFR
    Met1-Glu84
    Accession # P30047
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Simple Western
    10 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human and Mouse GCHFR by Western Blot. Western blot shows lysates of Hep3B human hepatocellular carcinoma cell line, Bowes human melanoma cell line, and XB2 mouse teratoma keratinocyte cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human GCHFR Monoclonal Antibody (Catalog # MAB7918) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for GCHFR at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human GCHFR by Simple WesternTM. Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line and Hep3B human hepatocellular carcinoma cell line, loaded at 0.5 mg/mL. A specific band was detected for GCHFR at approximately 11 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human GCHFR Monoclonal Antibody (Catalog # MAB7918). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GCHFR
GCHFR (GTP cyclohydrolase I feedback receptor), also called GFRP, is an 84 amino acid enzyme inhibitor that binds to and regulates the activity of GTP cyclohydrolase I. GTP cyclohydrolase I is the rate-limiting enzyme in biosynthesis of tetrahydrobiopterin, an essential cofactor for nitric oxide synthases and aromatic hydrolases. GCHFR is found as a 20 kDa homodimer in the nucleus and cytoplasm of multiple cell types, including endothelial cells, keratinocytes and melanocytes. Human GCHFR shares 93% amino acid sequence identity with mouse and rat GCHFR, which in turn share 100% identity with each other.
  • Long Name:
    GTP Cyclohydrolase I Feedback Regulator
  • Entrez Gene IDs:
    2644 (Human); 320415 (Mouse); 171128 (Rat)
  • Alternate Names:
    GCHFR; GFRP; GFRPP35; GTP cyclohydrolase I feedback regulator; GTP cyclohydrolase I feedback regulatory proteinGTP cyclohydrolase 1 feedback regulatory protein; HsT16933; MGC138467; MGC138469; p35
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Isotype Control

Ctrl MAB002 138  
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Mouse IgG1 Isotype Control

Ctrl MAB002R 1
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse IgG Antibody

WB AF007 4
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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