Human GCHFR Antibody

Catalog # Availability Size / Price Qty
MAB7918
MAB7918-SP
Detection of Human and Mouse GCHFR by Western Blot.
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Human GCHFR Antibody Summary

Species Reactivity
Human
Specificity
Detects human GCHFR in ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 846011
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human GCHFR
Met1-Glu84
Accession # P30047
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human and Mouse GCHFR antibody by Western Blot. View Larger

Detection of Human and Mouse GCHFR by Western Blot. Western blot shows lysates of Hep3B human hepatocellular carcinoma cell line, Bowes human melanoma cell line, and XB2 mouse teratoma keratinocyte cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human GCHFR Monoclonal Antibody (Catalog # MAB7918) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for GCHFR at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Human GCHFR antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human GCHFR by Simple WesternTM. Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line and Hep3B human hepatocellular carcinoma cell line, loaded at 0.5 mg/mL. A specific band was detected for GCHFR at approximately 11 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human GCHFR Monoclonal Antibody (Catalog # MAB7918). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GCHFR

GCHFR (GTP cyclohydrolase I feedback receptor), also called GFRP, is an 84 amino acid enzyme inhibitor that binds to and regulates the activity of GTP cyclohydrolase I. GTP cyclohydrolase I is the rate-limiting enzyme in biosynthesis of tetrahydrobiopterin, an essential cofactor for nitric oxide synthases and aromatic hydrolases. GCHFR is found as a 20 kDa homodimer in the nucleus and cytoplasm of multiple cell types, including endothelial cells, keratinocytes and melanocytes. Human GCHFR shares 93% amino acid sequence identity with mouse and rat GCHFR, which in turn share 100% identity with each other.

Long Name
GTP Cyclohydrolase I Feedback Regulator
Entrez Gene IDs
2644 (Human); 320415 (Mouse); 171128 (Rat)
Alternate Names
GCHFR; GFRP; GFRPP35; GTP cyclohydrolase I feedback regulator; GTP cyclohydrolase I feedback regulatory proteinGTP cyclohydrolase 1 feedback regulatory protein; HsT16933; MGC138467; MGC138469; p35

Product Datasheets

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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