Detection of Human and Mouse GCHFR by Western Blot.
Western blot shows lysates of Hep3B human hepatocellular carcinoma cell line, Bowes human melanoma cell line, and XB2 mouse teratoma keratinocyte cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human GCHFR Monoclonal Antibody (Catalog # MAB7918) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for GCHFR at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human GCHFR by Simple WesternTM.
Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line and Hep3B human hepatocellular carcinoma cell line, loaded at 0.5 mg/mL. A specific band was detected for GCHFR at approximately 11 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human GCHFR Monoclonal Antibody (Catalog # MAB7918). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
GCHFR (GTP cyclohydrolase I feedback receptor), also called GFRP, is an 84 amino acid enzyme inhibitor that binds to and regulates the activity of GTP cyclohydrolase I. GTP cyclohydrolase I is the rate-limiting enzyme in biosynthesis of tetrahydrobiopterin, an essential cofactor for nitric oxide synthases and aromatic hydrolases. GCHFR is found as a 20 kDa homodimer in the nucleus and cytoplasm of multiple cell types, including endothelial cells, keratinocytes and melanocytes. Human GCHFR shares 93% amino acid sequence identity with mouse and rat GCHFR, which in turn share 100% identity with each other.
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