Key Product Details

Species Reactivity

Human

Applications

Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 846011
View all GCHFR Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human GCHFR
Met1-Glu84
Accession # P30047

Specificity

Detects human GCHFR in ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human GCHFR Antibody

Detection of Human and Mouse GCHFR antibody by Western Blot.

Detection of Human and Mouse GCHFR by Western Blot.

Western blot shows lysates of Hep3B human hepatocellular carcinoma cell line, Bowes human melanoma cell line, and XB2 mouse teratoma keratinocyte cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human GCHFR Monoclonal Antibody (Catalog # MAB7918) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for GCHFR at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human GCHFR antibody by Simple WesternTM.

Detection of Human GCHFR by Simple WesternTM.

Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line and Hep3B human hepatocellular carcinoma cell line, loaded at 0.5 mg/mL. A specific band was detected for GCHFR at approximately 11 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human GCHFR Monoclonal Antibody (Catalog # MAB7918). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human GCHFR Antibody

Application
Recommended Usage

Simple Western

10 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line and Hep3B human hepatocellular carcinoma cell line

Western Blot

1 µg/mL
Sample: Hep3B human hepatocellular carcinoma cell line, Bowes human melanoma cell line, and XB2 mouse teratoma keratinocyte cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GCHFR

GCHFR (GTP cyclohydrolase I feedback receptor), also called GFRP, is an 84 amino acid enzyme inhibitor that binds to and regulates the activity of GTP cyclohydrolase I. GTP cyclohydrolase I is the rate-limiting enzyme in biosynthesis of tetrahydrobiopterin, an essential cofactor for nitric oxide synthases and aromatic hydrolases. GCHFR is found as a 20 kDa homodimer in the nucleus and cytoplasm of multiple cell types, including endothelial cells, keratinocytes and melanocytes. Human GCHFR shares 93% amino acid sequence identity with mouse and rat GCHFR, which in turn share 100% identity with each other.

Long Name

GTP Cyclohydrolase I Feedback Regulator

Alternate Names

GFRP, HsT16933, p35

Entrez Gene IDs

2644 (Human); 320415 (Mouse); 171128 (Rat)

Gene Symbol

GCHFR

UniProt

P30047 (Human)

Additional GCHFR Products

Product Documents for Human GCHFR Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human GCHFR Antibody

For research use only

Related Research Areas

Redox Enzymes

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