Human GFR alpha-3/GDNF R alpha-3 Antibody Summary
Accession # AAC24355
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Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human GFR alpha ‑3/GDNF R alpha ‑3 by Western Blot. Western blot shows lysates of human stomach tissue and human small intestine tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human GFRa-3/GDNF Ra-3 Monoclonal Antibody (Catalog # MAB6701) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for GFRa-3/GDNF Ra-3 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GFR alpha-3/GDNF R alpha-3
Glial cell line-derived growth factor (GDNF), neurturin (NTN), persephin (PEP) and artemin (ART), distant members of the TGF-beta superfamily, are neurotrophic factors for a variety of neuronal populations in the central and peripheral nervous systems. The bioactivities of these neurotrophic factors are mediated through a receptor complex composed of the non ligand-binding signaling subunit (c-Ret receptor tyrosine kinase) and one of four ligand binding cysteine-rich glycosyl-phosphatidylinositol (GPI)-linked cell surface proteins (GFR alpha -1, 2, 3, or 4). These four GFR-alpha proteins share conserved placements of many of their cysteine residues. GFR alpha -1, 2, 3, or 4 have been shown to preferentially bind GDNF, NTN, ART, and PSP, respectively. While the GFR alpha -3/Ret complex is highly specific for ART, the GFR alpha -1 /Ret complex is extremely promiscuous and may also be utilized by NTN and ART. Human GFR alpha -3 cDNA encodes a 400 amino acid (aa) precursor protein with an N-terminal signal peptide and C-terminal hydrophobic domain. GFR alpha -3 is approximately 34% and 36% identical to GFR alpha -1 and GFR alpha -2, respectively. Human and mouse GFR alpha -3 share approximately 76% aa sequence homology. The gene encoding GFR alpha -3 has been localized to human chromosome 5. GFR alpha -3 is expressed at high levels in the developing and adult sensory and sympathetic ganglia of the peripheral nervous system. It is also expressed in non-neural tissues and may show tissue-specific differences in molecular weight. In the trigeminal ganglions, the expression of GFR alpha -3 is found in a population of neurons distinct from those expressing GFR alpha -1 or 2.
- Baloh, R. et al. (1998) Neuron 21:1291.
- Baloh, R. et al. (1998) Proc. Natl. Acad. Sci. USA 95:5801.
- Worby, C. et al. (1998) J. Biol. Chem. 273:3502.
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