Human GLI-2 Antibody Summary
Accession # BAA25668
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of GLI‑2-regulated Genes by Chromatin Immunoprecipitation. Jurkat human acute T cell leukemia cell line treated with 50 ng/mL PMA and 200 ng/mL calcium ionomycin for 30 minutes was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. GLI-2/DNA complexes were immunoprecipitated using 5 µg Sheep Anti-Human GLI-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3526) or control antibody (Catalog # 5-001-A) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Sheep IgG Secondary Antibody (Catalog # [[catalogNumber:BAF016).]]Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (Catalog # [[catalogNumber:MAG999]]) and DNA was purified using chelating resin solution. Thebcl-2promoter was detected by standard PCR.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
GLI-2 is a 166 kDa biopotential transcription regulator of the hedgehog signaling pathway. It contains 5 conserved tandem C2H2 zinc finger domains that are flanked by a repression domain at the N-terminal region and an activation domain in the C-terminal region. At least four isoforms that differ in their N- or C-terminal regions have been described. Transcriptional activity of GLI-2 is regulated by proteolytic processing and degradation. Within the region used as immunogen, human and mouse GLI-2 share 88% amino acid sequence homology.
Citations for Human GLI-2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 3
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Regulation of GLI1 by cis DNA elements and epigenetic marks
Authors: R Taylor, J Long, JW Yoon, R Childs, KB Sylvesters, ML Nielsen, KF Leong, S Iannaccone, DO Walterhous, DJ Robbins, P Iannaccone
DNA Repair (Amst.), 2019;79(0):10-21.
Sample Types: Whole Cells
Applications: Proximity Ligation Assay
SOX2 regulates self-renewal and tumorigenicity of human melanoma-initiating cells.
Authors: Santini R, Pietrobono S, Pandolfi S, Montagnani V, D'Amico M, Penachioni J, Vinci M, Borgognoni L, Stecca B
Sample Types: Cell Lysates
GLI2 Transcription Factor Mediates Cytokine Cross-talk in the Tumor Microenvironment.
Authors: Elsawa SF, Almada LL, Ziesmer SC, Novak AJ, Witzig TE, Ansell SM, Fernandez-Zapico ME
J. Biol. Chem., 2011;286(24):21524-34.
Sample Types: Cell Lysates
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