Human GLI-2 Antibody

Catalog # Availability Size / Price Qty
AF3526
AF3526-SP
Detection of GLI‑2-regulated Genes by Chromatin Immunoprecipitation.
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Product Details
Citations (3)
FAQs
Supplemental Products
Reviews (3)

Human GLI-2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human GLI-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 25% cross-reactivity with recombinant mouse GLI-2 and 5% cross-reactivity with recombinant human (rh) GLI-1 and rhGLI-3 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human GLI-2
Ala2-Glu91
Accession # BAA25668
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human GLI‑2
Immunohistochemistry
5-15 µg/mL
Immersion fixed paraffin-embedded sections of human skin, basal cell carcinoma, and prostate cancer tissue
Chromatin Immunoprecipitation (ChIP)
5 µg/5 x 106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Chromatin Immunoprecipitation (ChIP) Detection of GLI-2-regulated Genes antibody by Chromatin Immunoprecipitation. View Larger

Detection of GLI‑2-regulated Genes by Chromatin Immunoprecipitation. Jurkat human acute T cell leukemia cell line treated with 50 ng/mL PMA and 200 ng/mL calcium ionomycin for 30 minutes was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. GLI-2/DNA complexes were immunoprecipitated using 5 µg Sheep Anti-Human GLI-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3526) or control antibody (Catalog # 5-001-A) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Sheep IgG Secondary Antibody (Catalog # [[catalogNumber:BAF016).]]Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (Catalog # [[catalogNumber:MAG999]]) and DNA was purified using chelating resin solution. Thebcl-2promoter was detected by standard PCR.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GLI-2

GLI-2 is a 166 kDa biopotential transcription regulator of the hedgehog signaling pathway. It contains 5 conserved tandem C2H2 zinc finger domains that are flanked by a repression domain at the N-terminal region and an activation domain in the C-terminal region. At least four isoforms that differ in their N- or C-terminal regions have been described. Transcriptional activity of GLI-2 is regulated by proteolytic processing and degradation. Within the region used as immunogen, human and mouse GLI-2 share 88% amino acid sequence homology.

Long Name
GLI-Kruppel family member GLI2
Entrez Gene IDs
2736 (Human); 14633 (Mouse); 304729 (Rat)
Alternate Names
GLI family zinc finger 2; GLI2; GLI-2; GLI-Kruppel family member GLI2; glioma-associated oncogene family zinc finger 2; HPE9tax helper protein 1; oncogene GLI2; tax helper protein 2; Tax helper protein; tax-responsive element-2 holding protein; tax-responsive element-25-bp sequence binding protein; THP; THP1; THP2; zinc finger protein GLI2

Product Datasheets

Citations for Human GLI-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Regulation of GLI1 by cis DNA elements and epigenetic marks
    Authors: R Taylor, J Long, JW Yoon, R Childs, KB Sylvesters, ML Nielsen, KF Leong, S Iannaccone, DO Walterhous, DJ Robbins, P Iannaccone
    DNA Repair (Amst.), 2019;79(0):10-21.
    Species: Human
    Sample Types: Whole Cells
    Applications: Proximity Ligation Assay
  2. SOX2 regulates self-renewal and tumorigenicity of human melanoma-initiating cells.
    Authors: Santini R, Pietrobono S, Pandolfi S, Montagnani V, D'Amico M, Penachioni J, Vinci M, Borgognoni L, Stecca B
    Oncogene, 2014;33(38):4697-708.
    Species: Human
    Sample Types: Cell Lysates
    Applications: ChIP
  3. GLI2 Transcription Factor Mediates Cytokine Cross-talk in the Tumor Microenvironment.
    Authors: Elsawa SF, Almada LL, Ziesmer SC, Novak AJ, Witzig TE, Ansell SM, Fernandez-Zapico ME
    J. Biol. Chem., 2011;286(24):21524-34.
    Species: Human
    Sample Types: Cell Lysates
    Applications: ChIP

FAQs

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Reviews for Human GLI-2 Antibody

Average Rating: 3.7 (Based on 3 Reviews)

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Human GLI-2 Antibody
By Anonymous on 03/11/2019
Application: Microarrays Sample Tested: EDTA Plasma Species: Human

Human GLI-2 Antibody
By Anonymous on 11/10/2018
Application: ELISA Sample Tested: Plasma Species: Human

Human GLI-2 Antibody
By Anonymous on 11/02/2018
Application: MiAr Sample Tested: EDTA Plasma Species: Human