Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Glucosaminyl (N-acetyl) Transferase 1/GCNT1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Glucosaminyl (N-acetyl) Transferase 1/GCNT1
Arg33-His428
Accession # NP_001481

Specificity

Detects human Glucosaminyl (N-acetyl) Transferase 1/GCNT1 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Glucosaminyl (N-acetyl) Transferase 1/GCNT1 Antibody

Detection of Human Glucosaminyl (N-acetyl) Transferase 1/GCNT1 antibody by Western Blot.

Detection of Human Glucosaminyl (N‑acetyl) Transferase 1/GCNT1 by Western Blot.

Western blot shows lysates of SW480 human colorectal adenocarcinoma cell line, TT human medullary thyroid cancer cell line, and Capan-1 human pancreatic adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Glucosaminyl (N-acetyl) Transferase 1/GCNT1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7248) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Glucosaminyl (N-acetyl) Transferase 1/GCNT1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Glucosaminyl (N-acetyl) Transferase 1/GCNT1 antibody in HT-29 human cell line by Immunocytochemistry (ICC).

Glucosaminyl (N-acetyl) Transferase 1/GCNT1 in HT‑29 human cell line.

Glucosaminyl (N-acetyl) Transferase 1/GCNT1 was detected in immersion fixed HT-29 human colon adenocarcinoma cell line using Sheep Anti-Human Glucosaminyl (N-acetyl) Transferase 1/GCNT1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7248) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Glucosaminyl (N-acetyl) Transferase 1/GCNT1 antibody in Human Bladder Cancer Tissue by Immunohistochemistry (IHC-P).

Glucosaminyl (N-acetyl) Transferase 1/GCNT1 in Human Bladder Cancer Tissue.

Glucosaminyl (N-acetyl) Transferase 1/GCNT1 was detected in immersion fixed paraffin-embedded sections of human bladder cancer tissue using Sheep Anti-Human Glucosaminyl (N-acetyl) Transferase 1/GCNT1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7248) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane in smooth muscle cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Glucosaminyl (N-acetyl) Transferase 1/GCNT1 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed HT‑29 human colon adenocarcinoma cell line

Immunohistochemistry

3-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human bladder cancer tissue

Western Blot

1 µg/mL
Sample: SW480 human colorectal adenocarcinoma cell line, TT human medullary thyroid cancer cell line, and Capan‑1 human pancreatic adenocarcinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Glucosaminyl (N-acetyl) Transferase 1/GCNT1

Mucin-type O-glycans are initiated with an O-GalNAc attachment to a serine or threonine in a polypeptide. The O-GalNAc residues are subsequently extended by various glycosyltransferases resulting in different types of O-glycans. Most O-glycans contain the core 1 structure, Gal beta 1-3GalNAc. Glucosaminyl (N-acetyl) Transferase 1 (GCNT1) converts the core 1 O-glycan to core 2 O-glycan, Gal beta 1-3(GlcNAc beta 1-6)GalNAc, via the addition of a GlcNAc residue (1, 2). Various ligand carbohydrates can be formed from core 2 branched oligosaccharides. For example, sialyl Lex in mucin‑type glycoproteins of blood cells can be formed from core 2 branched oligosaccharides (3, 4). The expression of GCNT1 was found to be associated with the progression of various types of cancer (5, 6, 7). The enzymatic activity of the recombinant GCNT1 is measured using a phosphatase-coupled method (8).

References

  1. Bierhuizen, M.F. (1993) Genes & Development 7:468.
  2. Yeh, J.C. et al. (1999) J. Biol. Chem. 274:3215.
  3. Hemmerich, S. et al. (1995) J. Biol. Chem. 270:12035.
  4. Wilkins, P.P. et al. (1996) J. Biol. Chem. 270:18732.
  5. Shimodaira, K. et al. (1997) Cancer Res. 57: 5201.
  6. Hatakyama, S. et al. (2010) Int. J. Cancer 127:1052.
  7. St Hill, C.A. et al. (2009) BMC Cancer 9:79.
  8. Wu, Z.L. et al. (2011) Glycobiology 21:727.

Long Name

Glucosaminyl (N-acetyl) Transferase 1 Core 2

Alternate Names

C2GNT-L, C2GNT1, Core 2 GNT, G6NT, NACGT2

Entrez Gene IDs

2650 (Human); 14537 (Mouse); 64043 (Rat)

Gene Symbol

GCNT1

UniProt

NP_001481

Additional Glucosaminyl (N-acetyl) Transferase 1/GCNT1 Products

Product Documents for Human Glucosaminyl (N-acetyl) Transferase 1/GCNT1 Antibody

Certificate of Analysis

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Product Specific Notices for Human Glucosaminyl (N-acetyl) Transferase 1/GCNT1 Antibody

For research use only

Related Research Areas

Glycobiology-related Enzymes

Citations for Human Glucosaminyl (N-acetyl) Transferase 1/GCNT1 Antibody

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Protocols

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