Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Intracellular Staining by Flow Cytometry

Cited:

Immunohistochemistry-Paraffin, Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 6804
View all GM-CSF Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human GM-CSF
Ala18-Glu144
Accession # P04141

Specificity

Detects human GM-CSF in ELISAs. In ELISAs, this antibody does not cross-react with recombinant mouse (rm) GM‑CSF, rrGM‑CSF, rhM‑CSF, rhG‑CSF, rhIL‑5‑R alpha, or rhIL‑3 R alpha.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human GM‑CSF PE‑conjugated Antibody

Detection of GM-CSF antibody in Human Dendritic Cells antibody by Flow Cytometry.

Detection of GM‑CSF in Human Dendritic Cells by Flow Cytometry.

Human dendritic cells treated with LPS, Recombinant Human TNF-a (Catalog # 210-TA), and Recombinant Human TGF-beta 1 (Catalog # 240-B) were stained with Mouse Anti-Human GM-CSF PE-conjugated Monoclonal Antibody (Catalog # IC615P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human GM‑CSF PE‑conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/106 cells
Sample: Human dendritic cells treated with LPS, Recombinant Human TNF‑ alpha (Catalog # 210-TA), and Recombinant Human TGF‑ beta 1 (Catalog # 240-B) were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: GM-CSF

GM-CSF is a growth factor produced by a variety of lymphoid cells. It binds to receptor heterodimers consisting of a GM-CSF-specific alpha chain and the common beta chain that is shared by the high affinity receptors for IL-3 and IL-5.

Long Name

Granulocyte Macrophage Growth Factor

Alternate Names

CSF-2, CSF2, GMCSF, Molgramostim, Sargramostim

Entrez Gene IDs

1437 (Human); 12981 (Mouse); 116630 (Rat); 397208 (Porcine); 403923 (Canine); 493805 (Feline)

Gene Symbol

CSF2

UniProt

P04141 (Human)

Additional GM-CSF Products

Product Documents for Human GM‑CSF PE‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human GM‑CSF PE‑conjugated Antibody

For research use only

Citations for Human GM‑CSF PE‑conjugated Antibody

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FAQs for Human GM‑CSF PE‑conjugated Antibody

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  • Q: Do you have a protocol for the isolation and treatment of dendritic cells used for IC flow staining with Human GM-CSF Antibody, catalog #s MAB615 and IC615?

    A: MagCellect Human CD14+ Cell Isolation Kit, catalog # MAGH105, is used to isolate CD14+ monocytes from PBMCs, as the starting population.  Monocytes are cultured in media with rhIL-4 (catalog # 204-IL) at 20 ng/ml and rhGM-CSF (catalog # 215-GM) at 50ng/ml. On day 6, add LPS at 20ng/ml, rhTNF-a (catalog # 210-TA) at 20ng/ml and rhIL-1b (catalog # 201-LB) at 20ng/ml to culture medium and incubate cells overnight. Collect cells for flow cytometric analysis on day 7.

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