Human GM130/GOLGA2 Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB81991-100
MAB81991-SP
Detection  of Human GM130/GOLGA2 by Western Blot.
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Human GM130/GOLGA2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human GM130/GOLGA2 in direct ELISAs and Western blots.
Source
Recombinant Monoclonal Rabbit IgG Clone # 2059C
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human GM130/GOLGA2
Met258-Gln606
Accession # Q08379
Formulation
Supplied as a solution in PBS containing BSA, Glycerol and Sodium Azide. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection  of Human GM130/GOLGA2 by Western Blot. View Larger

Detection of Human GM130/GOLGA2 by Western Blot. Western blot shows lysates of MCF‑7 human breast cancer cell line, ZR‑75 human breast cancer cell line, and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human GM130/GOLGA2 Monoclonal Antibody (Catalog # MAB81991) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for GM130/
GOLGA2 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 °C, as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 6 months, -20 to -70 °C under sterile conditions after opening.

Background: GM130/GOLGA2

GM130, also known as Golgin subfamily A member 2 and GOLGA2, is a 130 kDa member of the Golgin protein family.  GM130/GOLGA2 is a component of the Golgi membrane involved in maintaining cis-Golgi structure.  GM130/GOLGA2 protein is thought to play roles in the stacking of Golgi cisternae and in vesicular transport. Several alternatively spliced transcript variants of this gene have been predicted. Human GM130/GOLGA2 protein is 1002 amino acids (aa) in length, and over aa 528‑606, shares 90% sequence identity with its mouse and rat orthologs.

Long Name
Golgin A2
Entrez Gene IDs
2801 (Human); 99412 (Mouse)
Alternate Names
130 kDa cis-Golgi matrix protein; GM130 autoantigen; GM130; GM130Golgi matrix protein GM130; GOLGA2; golgi autoantigen, golgin subfamily a, 2; golgin A2; Golgin subfamily A member 2; Golgin-95; MGC20672; SY11 Protein

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