Human GM130/GOLGA2 Antibody

Recombinant Monoclonal Antibody..
  
  • Species Reactivity
    Human
  • Specificity
    Detects human GM130/GOLGA2 in direct ELISAs and Western blots.
  • Source
    Recombinant Monoclonal Rabbit IgG Clone # 2059C
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human GM130/GOLGA2
    Met258-Gln606
    Accession # Q08379
  • Formulation
    Supplied as a solution in PBS containing BSA, Glycerol and Sodium Azide. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human GM130/GOLGA2 by Western Blot. Western blot shows lysates of MCF‑7 human breast cancer cell line, ZR‑75 human breast cancer cell line, and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human GM130/GOLGA2 Monoclonal Antibody (Catalog # MAB81991) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for GM130/
GOLGA2 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 °C, as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after opening.
    • 6 months, -20 to -70 °C under sterile conditions after opening.
Background: GM130/GOLGA2
GM130, also known as Golgin subfamily A member 2 and GOLGA2, is a 130 kDa member of the Golgin protein family.  GM130/GOLGA2 is a component of the Golgi membrane involved in maintaining cis-Golgi structure.  GM130/GOLGA2 protein is thought to play roles in the stacking of Golgi cisternae and in vesicular transport. Several alternatively spliced transcript variants of this gene have been predicted. Human GM130/GOLGA2 protein is 1002 amino acids (aa) in length, and over aa 528‑606, shares 90% sequence identity with its mouse and rat orthologs.
  • Long Name:
    Golgin A2
  • Entrez Gene IDs:
    2801 (Human); 99412 (Mouse)
  • Alternate Names:
    130 kDa cis-Golgi matrix protein; GM130 autoantigen; GM130/GOLGA2; GM130Golgi matrix protein GM130; GOLGA2; golgi autoantigen, golgin subfamily a, 2; golgin A2; Golgin subfamily A member 2; Golgin-95; MGC20672; SY11 Protein
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Rabbit IgG Control

Ctrl AB-105-C 26  
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Molecular Weight Markers
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Full-Range Molecular Weight Marker, Biotinylated

MW001
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Secondary Antibodies
Description Application Cat# Citations Images  

Rabbit IgG Horseradish Peroxidase-conjugated Antibody

WB HAF008 12  
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Rabbit IgG PE-conjugated Antibody

Flow F0110 1  
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Rabbit IgG APC-conjugated Antibody

Flow F0111 1  
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Donkey Anti-Rabbit IgG NL557 Affinit Purified PAb

Flow, IHC, ICC NL004 6
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Donkey Anti-Rabbit IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL005 2
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Donkey Anti-Rabbit IgG NL493 Affinity Purified PAb

Flow, IHC, ICC NL006 4
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Goat Anti-Rabbit IgG Biotinylated Antibody

WB BAF008 2
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Rabbit IgG Fluorescein-conjugated Antibody

Flow F0112 2  
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Rabbit IgG Antibody

WB AF008 1
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Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC003  
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Donkey Anti-Rabbit IgG (H+L) Antibody

D-301-C-ABS2
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