Human GPR107 Antibody

Catalog # Availability Size / Price Qty
MAB10926-100
MAB10926-SP
Detection of Human GPR107 by Western Blot.
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Human GPR107 Antibody Summary

Species Reactivity
Human
Specificity
Detects human GPR107 in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 1040717
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human GPR107 synthetic peptide
Accession # Q5VW38
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
AGS human gastric adenocarcinoma cell line, KATO‑III human gastric carcinoma cell line, and PANC‑1 human pancreatic carcinoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot View Larger

Detection of Human GPR107 by Western Blot. Western blot shows lysates of non-boiled AGS human gastric adenocarcinoma cell line, KATO‑III human gastric carcinoma cell line, and PANC‑1 human pancreatic carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human GPR107 Monoclonal Antibody (Catalog # MAB10926) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for GPR107 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

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Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GPR107

Human GPR107 is an ‘orphan' 7-transmembrane receptor from the G protein-coupled receptor (GPCR) family of proteins.

GPR107 has been proposed to act as a receptor for Neuronostatin, a peptide derived from the somatostatin/SST precursor. Neuronostatin acts via GPR107 to increase cAMP-independent PKA phosphorylation and proglucagon mRNA accumulation in pancreatic alpha -cells.

Long Name
G Protein-coupled Receptor 107
Entrez Gene IDs
57720 (Human); 277463 (Mouse); 311857 (Rat)
Alternate Names
BA138E2.2; DKFZp667C222; FLJ16312; FLJ20998; FLJ22591; G protein-coupled receptor 107; GPR107; KIAA1624; KIAA1624GCDRP; Lung seven transmembrane receptor 1; LUSTR1; LUSTR1MGC126118; MGC15440; protein GPR107; RP11-88G17

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