Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1048103
View all GPR81 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to the N-term sequences of Human HCAR1/GPR81

Specificity

Detects human HCAR1/GPFR81 peptide in direct Elisa

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human GPR81 Antibody

Detection of Human GPR81 by Western Blot.

Western blot shows lysates of A549 human lung carcinoma cell line, and HT-29 human colon adenocarcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human GPR81 Monoclonal Antibody (Catalog # MAB11134) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for GPR81 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human GPR81 Antibody

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: A549 human lung carcinoma cell line, and HT-29 human colon adenocarcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GPR81

Human Hydroxycarboxylic acid receptor 1 (HCAR1), also known as GPR81 and GPR194, is a G-protein coupled receptor (GPCR) thatt acts as a receptor for L-lactate and mediates its anti-lipolytic effect through a G(i)-protein-mediated pathway.

Long Name

G Protein-coupled Receptor 81

Alternate Names

FKSG80, GPR104, HCA1, HCAR1, LACR1, TA-GPCR

Entrez Gene IDs

27198 (Human); 243270 (Mouse); 689936 (Rat)

Gene Symbol

HCAR1

Additional GPR81 Products

Product Documents for Human GPR81 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human GPR81 Antibody

For research use only

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