Human GPR81 Antibody

Catalog # Availability Size / Price Qty
MAB11134-100
MAB11134-SP

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Detection of Human GPR81 by Western Blot.
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Human GPR81 Antibody Summary

Species Reactivity
Human
Specificity
Detects human HCAR1/GPFR81 peptide in direct Elisa
Source
Monoclonal Mouse IgG1 Clone # 1048103
Purification
Protein A or G purified from ascites
Immunogen
Synthetic peptide corresponding to the N-term sequences of Human HCAR1/GPR81
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
A549 human lung carcinoma cell line, and HT-29 human colon adenocarcinoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot View Larger

Detection of Human GPR81 by Western Blot. Western blot shows lysates of A549 human lung carcinoma cell line, and HT-29 human colon adenocarcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human GPR81 Monoclonal Antibody (Catalog # MAB11134) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for GPR81 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GPR81

Human Hydroxycarboxylic acid receptor 1 (HCAR1), also known as GPR81 and GPR194, is a G-protein coupled receptor (GPCR) thatt acts as a receptor for L-lactate and mediates its anti-lipolytic effect through a G(i)-protein-mediated pathway.

Long Name
G Protein-coupled Receptor 81
Entrez Gene IDs
27198 (Human); 243270 (Mouse); 689936 (Rat)
Alternate Names
FKSG80; G protein-coupled receptor 104; G protein-coupled receptor 81; GPR104; GPR81; G-protein coupled receptor 104; G-protein coupled receptor 81; HCA1; HCAR1; LACR1; lactate receptor 1; TA-GPCR; T-cell activation G protein-coupled receptor

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