Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 566133
View all GPR83 Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human GPR83
Accession # Q9NYM4

Specificity

Detects human GPR83 in direct ELISAs

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human GPR83 Antibody

Detection of GPR83 in HEK293 Human Cell Line Transfected with Human GPR83 and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with (A) human GPR83 or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human GPR83 Monoclonal Antibody (Catalog # MAB10943) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). Quadrant markers were set based on Mouse IgG1 Isotype Control (MAB002). Staining was performed using our Staining Membrane-associated Proteins protocol.

Applications for Human GPR83 Antibody

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: HEK293 Human Cell Line Transfected with Human GPR83 and eGFP

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Advanced Features

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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GPR83

GPR83 is an orphan G protein-coupled receptor most homologous to the tachykinin and neuropeptide Y receptors. GPR83 is involved in the conversion of naive CD4+CD25− T-cells into CD4+Foxp3+ Treg cells during inflammatory immune responses in vivo.

Long Name

G Protein-coupled Receptor 83

Alternate Names

GIR, GPR72, RP39, RP82

Entrez Gene IDs

10888 (Human); 14608 (Mouse); 140595 (Rat)

Gene Symbol

GPR83

UniProt

Q9NYM4 (Human)

Additional GPR83 Products

Product Documents for Human GPR83 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human GPR83 Antibody

For research use only

Related Research Areas

Nutrition and Appetite
Obesity

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