Human GPR83 Antibody

Catalog #: MAB10943 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB10943-100
MAB10943-SP

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Detection of GPR83 in HEK293 Human Cell Line Transfected with Human GPR83 and eGFP by Flow Cytometry.

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Human GPR83 Antibody Summary

Species Reactivity

Human

Specificity

Detects human GPR83 in direct ELISAs

Source

Monoclonal Mouse IgG₁ Clone # 566133

Purification

Protein A or G purified from cell culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human GPR83
Accession # Q9NYM4

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

HEK293 Human Cell Line Transfected with Human GPR83 and eGFP

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of GPR83 in HEK293 Human Cell Line Transfected with Human GPR83 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human GPR83 or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human GPR83 Monoclonal Antibody (Catalog # MAB10943) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). Quadrant markers were set based on Mouse IgG1 Isotype Control (MAB002). Staining was performed using our Staining Membrane-associated Proteins protocol.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GPR83

GPR83 is an orphan G protein-coupled receptor most homologous to the tachykinin and neuropeptide Y receptors. GPR83 is involved in the conversion of naive CD4+CD25− T-cells into CD4+Foxp3+ Treg cells during inflammatory immune responses in vivo.

Long Name

G Protein-coupled Receptor 83

Entrez Gene IDs

10888 (Human); 14608 (Mouse); 140595 (Rat)

Alternate Names

G protein-coupled receptor 72; G protein-coupled receptor 83; GIR; GPR72; GPR72glucocorticoid induced receptor; GPR83; G-protein coupled receptor 72; KIAA1540; probable G-protein coupled receptor 83; RP39; RP82