Human GPR83 Antibody

Catalog # Availability Size / Price Qty
MAB10943-100
MAB10943-SP
Detection of GPR83 in HEK293 Human Cell Line Transfected with Human GPR83 and eGFP by Flow Cytometry.
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Human GPR83 Antibody Summary

Species Reactivity
Human
Specificity
Detects human GPR83 in direct ELISAs
Source
Monoclonal Mouse IgG1 Clone # 566133
Purification
Protein A or G purified from cell culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human GPR83
Accession # Q9NYM4
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
HEK293 Human Cell Line Transfected with Human GPR83 and eGFP

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry View Larger

Detection of GPR83 in HEK293 Human Cell Line Transfected with Human GPR83 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human GPR83 or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human GPR83 Monoclonal Antibody (Catalog # MAB10943) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). Quadrant markers were set based on Mouse IgG1 Isotype Control (MAB002). Staining was performed using our Staining Membrane-associated Proteins protocol.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GPR83

GPR83 is an orphan G protein-coupled receptor most homologous to the tachykinin and neuropeptide Y receptors. GPR83 is involved in the conversion of naive CD4+CD25− T-cells into CD4+Foxp3+ Treg cells during inflammatory immune responses in vivo.

Long Name
G Protein-coupled Receptor 83
Entrez Gene IDs
10888 (Human); 14608 (Mouse); 140595 (Rat)
Alternate Names
G protein-coupled receptor 72; G protein-coupled receptor 83; GIR; GPR72; GPR72glucocorticoid induced receptor; GPR83; G-protein coupled receptor 72; KIAA1540; probable G-protein coupled receptor 83; RP39; RP82

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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