Human GPR97 Antibody Summary
Accession # Q86Y34
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human GPR97 by Western Blot. Western blot shows lysates of HEK293T human embryonic kidney cell line either mock transfected or transfected with human GPR97 and eGFP. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human GPR97 Monoclonal Antibody (Catalog # MAB10097) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for GPR97 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of GPR97 in HEK293 Human Cell Line Transfected with Human GPR97 and eGFP by Flow Cytometry HEK293 human embryonic kidney cell line transfected with human GPR97 and eGFP was stained with (A) Rabbit Anti-Human GPR97 Monoclonal Antibody (Catalog # MAB10097) or (B) Rabbit IgG control antibody (Catalog # MAB1050) followed by APC-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Adhesion G protein-coupled receptor G3 (ADGRG3, GPR97 or PGR26) is an orphan GPCR receptor that in vitro regulates migration of lymphatic endothelial cells via the small GTPases RhoA and CDC42. GPR97 is a 549 aminoacids (aa) 7-transmembrane domain (7TM) receptor belonging to the secretin sub-family of GPCR. Human GPR97 is expressed in granulocytes and endothelial cells of the vasculature.
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