Human GPR97 Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB10097-100
MAB10097-SP
Detection  of Human GPR97 by Western Blot.
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Human GPR97 Antibody Summary

Species Reactivity
Human
Specificity
Detects human GPR97 in direct ELISAs.
Source
Recombinant Monoclonal Rabbit IgG Clone # 2435C
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Human embryonic kidney cell, HEK293-transfected human GPR97
Met1-Leu249
Accession # Q86Y34
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection  of Human GPR97 by Western Blot. View Larger

Detection of Human GPR97 by Western Blot. Western blot shows lysates of HEK293T human embryonic kidney cell line either mock transfected or transfected with human GPR97 and eGFP. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human GPR97 Monoclonal Antibody (Catalog # MAB10097) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for GPR97 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of GPR97 in HEK293 Human Cell Line Transfected with Human GPR97 and eGFP by Flow Cytometry View Larger

Detection of GPR97 in HEK293 Human Cell Line Transfected with Human GPR97 and eGFP by Flow Cytometry HEK293 human embryonic kidney cell line transfected with human GPR97 and eGFP was stained with (A) Rabbit Anti-Human GPR97 Monoclonal Antibody (Catalog # MAB10097) or (B) Rabbit IgG control antibody (Catalog # MAB1050) followed by APC-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GPR97

Adhesion G protein-coupled receptor G3 (ADGRG3, GPR97 or PGR26) is an orphan GPCR receptor that in vitro regulates migration of lymphatic endothelial cells via the small GTPases RhoA and CDC42. GPR97 is a 549 aminoacids (aa) 7-transmembrane domain (7TM) receptor belonging to the secretin sub-family of GPCR. Human GPR97 is expressed in granulocytes and endothelial cells of the vasculature.

 

Long Name
G Protein-coupled Receptor 97
Entrez Gene IDs
222487 (Human); 54672 (Mouse); 291854 (Rat)
Alternate Names
G protein-coupled receptor 97; GPR97; PB99; PGR26; PGR26G-protein coupled receptor PGR26; probable G-protein coupled receptor 97

Product Datasheets

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