|Detection of Human HE4/WFDC2 by Western Blot. Western blot shows lysates of HT‑29 human colon adenocarcinoma cell line and LNCaP human prostate cancer cell line. PVDF Membrane was probed with 0.5 µg/mL of Sheep Anti-Human HE4/WFDC2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6274) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for HE4/WFDC2 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.|
HE4 (also WFDC2; WAP Four-disulfide core domain protein 2) is a secreted, 25 kDa glycoprotein member of the Whey Acidic Protein family of molecules. It is expressed by a wide variety of epithelial cells, including respiratory epithelium, salivary gland mucous cells, breast duct epithelium, distal tubule renal epithelium, and epididymal epithelium. It is believed to play a role in innate defense, and/or function as a protease inhibitor (perhaps for neutrophil elastase). Although ovarian cuboidal epithelium does not express HE4, ovarian epithelial tumors do, leading to the suggestion that HE4 could represent a tumor marker for ovarian cancer. Mature human HE4 is 94 amino acids (aa) in length. It contains two WAP domains that likely mediate antiprotease and/or antimicrobial activity (aa 31‑73 and 74‑123). There are four potential splice variants. One shows a deletion of aa 27‑74, while three others show aa substitutions: 28 aa for aa 75‑124, 23 aa for aa 1‑74, and 10 aa for aa 71‑124. Over aa 31‑124, human HE4 shares 63% aa identity with an extended (144 amino acid) mouse form of mature HE4.
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