Human HE4/WFDC2 Antibody

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Human HE4/WFDC2 ELISA Standard Curve.
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Product Details
Citations (1)
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Human HE4/WFDC2 Antibody Summary

Species Reactivity
Detects human HE4/WFDC2 in direct ELISAs.
Polyclonal Sheep IgG
Antigen Affinity-purified
Mouse myeloma cell line NS0-derived recombinant human HE4/WFDC2
Accession # Q14508
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human HE4/WFDC2 Monoclonal Antibody (Catalog # MAB62741).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human HE4/WFDC2 DuoSet ELISA Kit (Catalog # DY6274-05) for convenient development of a sandwich ELISA or the Human HE4/WFDC2 Quantikine ELISA Kit (Catalog # DHE400) for a complete optimized ELISA.

25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human HE4/WFDC2, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Human HE4/WFDC2 ELISA Standard Curve. Recombinant Human HE4/WFDC2 protein was serially diluted 2-fold and captured by Mouse Anti-Human HE4/WFDC2 Monoclonal Antibody (Catalog # MAB62741) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Human HE4/WFDC2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6274) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

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Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HE4/WFDC2

HE4 (also WFDC2; WAP Four-disulfide core domain protein 2) is a secreted, 25 kDa glycoprotein member of the Whey Acidic Protein family of molecules. It is expressed by a wide variety of epithelial cells, including respiratory epithelium, salivary gland mucous cells, breast duct epithelium, distal tubule renal epithelium, and epididymal epithelium. It is believed to play a role in innate defense, and/or function as a protease inhibitor (perhaps for neutrophil elastase). Although ovarian cuboidal epithelium does not express HE4, ovarian epithelial tumors do, leading to the suggestion that HE4 could represent a tumor marker for ovarian cancer. Mature human HE4 is 94 amino acids (aa) in length. It contains two WAP domains that likely mediate antiprotease and/or antimicrobial activity (aa 31‑73 and 74‑123). There are four potential splice variants. One shows a deletion of aa 27‑74, while three others show aa substitutions: 28 aa for aa 75‑124, 23 aa for aa 1‑74, and 10 aa for aa 71‑124. Over aa 31‑124, human HE4 shares 63% aa identity with an extended (144 amino acid) mouse form of mature HE4.

Long Name
WAP Four-disulfide Core Domain protein 2
Entrez Gene IDs
10406 (Human); 67701 (Mouse); 286888 (Rat)
Alternate Names
dJ461P17.6; EDDM4; Epididymal secretory protein E4; epididymis-specific, whey-acidic protein type, four-disulfide core; HE4; HE4MGC57529; Major epididymis-specific protein E4; Putative protease inhibitor WAP5; WAP domain containing protein HE4-V4; WAP four-disulfide core domain 2; WAP four-disulfide core domain protein 2; WAP5; WAP5epididymal protein 4; WFDC2

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Citation for Human HE4/WFDC2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Recurrence monitoring for ovarian cancer using a cell phone-integrated paper device to measure the ovarian cancer biomarker HE4/CRE ratio in urine
    Authors: EC Kight, I Hussain, AK Bowden, FR Haselton
    Scientific Reports, 2021-11-09;11(1):21945.
    Species: Human
    Sample Types: Urine
    Applications: ELISA Capture


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