Human HE4/WFDC2 Antibody Summary
Accession # Q14508
This antibody functions as an ELISA capture antibody when paired with Sheep Anti-Human HE4/WFDC2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6274).
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human HE4/WFDC2 DuoSet ELISA Kit (Catalog # DY6274-05) for convenient development of a sandwich ELISA or the Human HE4/WFDC2 Quantikine ELISA Kit (Catalog # DHE400) for a complete optimized ELISA.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Human HE4/WFDC2 ELISA Standard Curve. Recombinant Human HE4/WFDC2 protein was serially diluted 2-fold and captured by Mouse Anti-Human HE4/WFDC2 Monoclonal Antibody (Catalog # MAB62741) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Human HE4/WFDC2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6274) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
HE4 (also WFDC2; WAP Four-disulfide core domain protein 2) is a secreted, 25 kDa glycoprotein member of the Whey Acidic Protein family of molecules. It is expressed by a wide variety of epithelial cells, including respiratory epithelium, salivary gland mucous cells, breast duct epithelium, distal tubule renal epithelium, and epididymal epithelium. It is believed to play a role in innate defense, and/or function as a protease inhibitor (perhaps for neutrophil elastase). Ovarian epithelial tumors have an increased expression of HE4, leading to the suggestion that HE4 could represent a tumor marker for ovarian cancer. Mature human HE4 is 94 amino acids (aa) in length. It contains two WAP domains that likely mediate antiprotease and/or antimicrobial activity (aa 31‑73 and 74‑123). There are four potential splice variants. One shows a deletion of aa 27‑74, while three others show aa substitutions: 28 aa for aa 75‑124, 23 aa for aa 1‑74, and 10 aa for aa 71‑124. Over aa 31‑124, human HE4 shares 63% aa identity with an extended (144 amino acid) mouse form of mature HE4.
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