Human HE4/WFDC2 Antibody

Catalog # Availability Size / Price Qty
MAB62741-100
MAB62741-SP
Human  HE4/WFDC2 ELISA Standard Curve.
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Human HE4/WFDC2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human HE4/WFDC2 in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 676009
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human HE4/WFDC2
Thr28-Phe124
Accession # Q14508
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
ELISA

This antibody functions as an ELISA capture antibody when paired with Sheep Anti-Human HE4/WFDC2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6274).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human HE4/WFDC2 DuoSet ELISA Kit (Catalog # DY6274-05) for convenient development of a sandwich ELISA or the Human HE4/WFDC2 Quantikine ELISA Kit (Catalog # DHE400) for a complete optimized ELISA.

 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Human HE4/WFDC2 ELISA Standard Curve. Recombinant Human HE4/WFDC2 protein was serially diluted 2-fold and captured by Mouse Anti-Human HE4/WFDC2 Monoclonal Antibody (Catalog # MAB62741) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Human HE4/WFDC2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6274) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HE4/WFDC2

HE4 (also WFDC2; WAP Four-disulfide core domain protein 2) is a secreted, 25 kDa glycoprotein member of the Whey Acidic Protein family of molecules. It is expressed by a wide variety of epithelial cells, including respiratory epithelium, salivary gland mucous cells, breast duct epithelium, distal tubule renal epithelium, and epididymal epithelium. It is believed to play a role in innate defense, and/or function as a protease inhibitor (perhaps for neutrophil elastase). Ovarian epithelial tumors have an increased expression of HE4, leading to the suggestion that HE4 could represent a tumor marker for ovarian cancer. Mature human HE4 is 94 amino acids (aa) in length. It contains two WAP domains that likely mediate antiprotease and/or antimicrobial activity (aa 31‑73 and 74‑123). There are four potential splice variants. One shows a deletion of aa 27‑74, while three others show aa substitutions: 28 aa for aa 75‑124, 23 aa for aa 1‑74, and 10 aa for aa 71‑124. Over aa 31‑124, human HE4 shares 63% aa identity with an extended (144 amino acid) mouse form of mature HE4.

Long Name
WAP Four-disulfide Core Domain protein 2
Entrez Gene IDs
10406 (Human); 67701 (Mouse); 286888 (Rat)

Product Datasheets

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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