Detection of Helios in Human PBMCs Stimulated to Induce Tregs by Flow Cytometry.
Human peripheral blood mononuclear cells (PBMCs) stimulated to induce Regulatory T Cells (Tregs) treated with 10 μg/mL Anti-CD3, 5 μg/mL Anti-CD28, 10 ng/mL Recombinant Human TGF‑ beta 1 (Catalog # 240-B), and 20 ng/mL Recombinant Human IL‑2 (Catalog # 202-IL) overnight were stained with Mouse Anti-Human CD25/IL-2 R alpha Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB1020G) and either (A) Mouse Anti-Human Helios PE‑conjugated Monoclonal Antibody (Catalog # IC73092P) or (B) Mouse IgG1 Phycoerythrin Isotype Control (Catalog # IC002P). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Helios, also known as IKZF2, is a 70 kDa DNA-binding transcription regulator in the Ikaros family that contains four N-terminal C2H2-type zinc finger domains (aa 112-219) and two C-terminal zinc finger domains (aa 471-523). Helios is expressed in developing hematopoietic and epithelial tissues and in adult T cells and thymic-derived regulatory T cells (Treg). It forms homodimers and also heterodimers with other Ikaros family proteins Ikaros, Pegasus, Eos, and Aiolos. Alternate splicing of human Helios generates a short isoform that lacks three of the the N-terminal zinc finger domains. This isoform is overexpressed in T cell leukemias where it can still dimerize with Ikaros proteins but functions as a dominant negative regulator. Within aa 1-97, human and mouse Helios share 96% aa sequence identity.
DNA-binding Protein Helios
Entrez Gene IDs:
22807 (Human); 22779 (Mouse)
Helios; IKAROS family zinc finger 2 (Helios); Ikaros family zinc finger protein 2; IKZF2; MGC34330; subfamily 1A, 2 (Helios); zinc finger DNA binding protein Helios; zinc finger protein Helios; ZNF1A2; ZNFN1A2
Have you used Human Helios PE-conjugated Antibody?
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