Human Helios PE-conjugated Antibody

IC73092P has been discontinued. View all Helios products.
  
  • Species Reactivity
    Human
  • Specificity
    Detects human Helios in direct ELISAs.
  • Source
    Monoclonal Mouse IgG1 Clone # 736440
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human Helios
    Met1-Gln97
    Accession # Q9UKS7
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of Helios in Human PBMCs Stimulated to Induce Tregs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) stimulated to induce Regulatory T Cells (Tregs) treated with 10 μg/mL Anti-CD3, 5 μg/mL Anti-CD28, 10 ng/mL Recombinant Human TGF‑ beta 1 (Catalog # 240-B), and 20 ng/mL Recombinant Human IL‑2 (Catalog # 202-IL) overnight were stained with Rabbit Anti-Human/Mouse FoxP3 Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # IC8214R) and either (A) Mouse Anti-Human Helios PE‑conjugated Monoclonal Antibody (Catalog # IC73092P) or (B) Mouse IgG1 Phycoerythrin Isotype Control (Catalog # IC002P).  To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012).  View our protocol for Staining Intracellular Molecules.
Intracellular Staining by Flow Cytometry
Detection of Helios in Human PBMCs Stimulated to Induce Tregs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) stimulated to induce Regulatory T Cells (Tregs) treated with 10 μg/mL Anti-CD3, 5 μg/mL Anti-CD28, 10 ng/mL Recombinant Human TGF‑ beta 1 (Catalog # 240-B), and 20 ng/mL Recombinant Human IL‑2 (Catalog # 202-IL) overnight were stained with Mouse Anti-Human CD25/IL-2 R alpha Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB1020G) and either (A) Mouse Anti-Human Helios PE‑conjugated Monoclonal Antibody (Catalog # IC73092P) or (B) Mouse IgG1 Phycoerythrin Isotype Control (Catalog # IC002P).  To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Helios
Helios, also known as IKZF2, is a 70 kDa DNA-binding transcription regulator in the Ikaros family that contains four N-terminal C2H2-type zinc finger domains (aa 112-219) and two C-terminal zinc finger domains (aa 471-523). Helios is expressed in developing hematopoietic and epithelial tissues and in adult T cells and thymic-derived regulatory T cells (Treg). It forms homodimers and also heterodimers with other Ikaros family proteins Ikaros, Pegasus, Eos, and Aiolos. Alternate splicing of human Helios generates a short isoform that lacks three of the the N-terminal zinc finger domains. This isoform is overexpressed in T cell leukemias where it can still dimerize with Ikaros proteins but functions as a dominant negative regulator. Within aa 1-97, human and mouse Helios share 96% aa sequence identity.
  • Long Name:
    DNA-binding Protein Helios
  • Entrez Gene IDs:
    22807 (Human); 22779 (Mouse)
  • Alternate Names:
    Helios; IKAROS family zinc finger 2 (Helios); Ikaros family zinc finger protein 2; IKZF2; MGC34330; subfamily 1A, 2 (Helios); zinc finger DNA binding protein Helios; zinc finger protein Helios; ZNF1A2; ZNFN1A2
Related Research Areas

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