Human Helios PE-conjugated Antibody
Human Helios PE-conjugated Antibody Summary
Met1-Gln97
Accession # Q9UKS7
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Helios in Human PBMCs Stimulated to Induce Tregs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) stimulated to induce Regulatory T Cells (Tregs) treated with 10 µg/mL Anti-CD3, 5 µg/mL Anti-CD28, 10 ng/mL Recombinant Human TGF-beta 1 (Catalog # 240-B), and 20 ng/mL Recombinant Human IL-2 (Catalog # 202-IL) overnight were stained with Rabbit Anti-Human/Mouse FoxP3 Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # IC8214R) and either (A) Mouse Anti-Human Helios PE-conjugated Monoclonal Antibody (Catalog # IC73092P) or (B) Mouse IgG1Phycoerythrin Isotype Control (Catalog # IC002P). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
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Detection of Helios in Human PBMCs Stimulated to Induce Tregs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) stimulated to induce Regulatory T Cells (Tregs) treated with 10 µg/mL Anti-CD3, 5 µg/mL Anti-CD28, 10 ng/mL Recombinant Human TGF-beta 1 (Catalog # 240-B), and 20 ng/mL Recombinant Human IL-2 (Catalog # 202-IL) overnight were stained with Mouse Anti-Human CD25/IL-2 R alpha Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB1020G) and either (A) Mouse Anti-Human Helios PE-conjugated Monoclonal Antibody (Catalog # IC73092P) or (B) Mouse IgG1Phycoerythrin Isotype Control (Catalog # IC002P). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Helios
Helios, also known as IKZF2, is a 70 kDa DNA-binding transcription regulator in the Ikaros family that contains four N-terminal C2H2-type zinc finger domains (aa 112-219) and two C-terminal zinc finger domains (aa 471-523). Helios is expressed in developing hematopoietic and epithelial tissues and in adult T cells and thymic-derived regulatory T cells (Treg). It forms homodimers and also heterodimers with other Ikaros family proteins Ikaros, Pegasus, Eos, and Aiolos. Alternate splicing of human Helios generates a short isoform that lacks three of the the N-terminal zinc finger domains. This isoform is overexpressed in T cell leukemias where it can still dimerize with Ikaros proteins but functions as a dominant negative regulator. Within aa 1-97, human and mouse Helios share 96% aa sequence identity.
Product Datasheets
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