Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # 24212
View all CD25/IL-2R alpha Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD25/IL-2 R alpha
Glu22-Cys213
Accession # P01589

Specificity

Detects human CD25/IL-2 R alpha in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL‑2 R gamma or rhIL‑15 R alpha is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human CD25/IL-2R alpha Alexa Fluor® 488-conjugated Antibody

Detection of CD25/IL-2 R alpha antibody in Human PBMCs antibody by Flow Cytometry.

Detection of CD25/IL-2 R alpha in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) treated with 20 ng/mL Recombinant Human IL-2 (Catalog # 202-IL) and 10 ng/mL Recombinant Human TGF-beta 1 (Catalog # 240-B) for 2 days were stained with Mouse Anti-Human CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB3791A) and either (A) Mouse Anti-Human CD25/IL-2 R alpha Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB1020G) or (B) Mouse IgG2AAlexa Fluor 488 Isotype Control (Catalog # IC003G). View our protocol for Staining Membrane-associated Proteins.

Applications for Human CD25/IL-2R alpha Alexa Fluor® 488-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

5 µL/106 cells
Sample: Human peripheral blood mononuclear cells (PBMCs) treated with Recombinant Human IL‑2 (Catalog # 202-IL) and Recombinant Human TGF‑ beta 1 (Catalog # 240-B)

Reviewed Applications

Read 1 review rated 5 using FAB1020G in the following applications:

Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD25/IL-2R alpha

Human CD25, also known as IL-2 receptor alpha (IL-2 R alpha ) and asTac antigen, was initially identified as a 55 kDa membrane glycoprotein that is capable of binding IL‑2. The IL-2 R alpha cDNA encodes a 272 amino acid residue precursor Type I membrane protein with a 21 residue signal peptide, a 219 residue extracellular region, a 19 residue transmembrane region and a 13 residue cytoplasmic domain. IL-2 R alpha lacks structural features characteristic of members of the cytokine receptor superfamily. By itself, IL-2 R alpha binds IL-2 with low affinity. However, when IL-2 R alpha is associated with the IL-2 receptor beta and gamma chains, a high affinity heterotrimeric receptor complex that transduces IL-2 signals is formed. Soluble forms of many cytokine receptors have been reported, and a soluble form of IL-2 R alpha (IL-2 sR alpha ) appears in serum, concomitant with its increased expression on cells. The function of the soluble IL-2 R alpha is unclear. Increased levels of IL-2 sR alpha in biological fluids reportedly correlate with increased T and B cell activation and immune system activation. Increased serum concentration of IL-2 sR alpha has been observed in patients with a variety of inflammatory conditions and in the course of some leukemias and lymphomas.

Long Name

Interleukin 2 Receptor alpha

Alternate Names

CD25, IL-2 R alpha, IL-2Ra, IL2R alpha, IL2RA

Entrez Gene IDs

3559 (Human); 16184 (Mouse); 25704 (Rat); 403870 (Canine); 102123605 (Cynomolgus Monkey)

Gene Symbol

IL2RA

UniProt

P01589

Additional CD25/IL-2R alpha Products

Product Documents for Human CD25/IL-2R alpha Alexa Fluor® 488-conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CD25/IL-2R alpha Alexa Fluor® 488-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

Citations for Human CD25/IL-2R alpha Alexa Fluor® 488-conjugated Antibody

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  • Human CD25/IL-2 R alpha Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: CD8 T lymphocytes
    Species: Human
    Verified Customer | Posted 01/31/2019
    Human CD8+ T-cells were isolated from PBMCs and cultured with IL-2 overnight. Stained with anti-CD25 Alexa Fluor 488 (BL1).
    Human CD25/IL-2R alpha Alexa Fluor® 488-conjugated Antibody FAB1020G

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