Human Hepcidin DuoSet ELISA

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Catalog # Availability Size / Price Qty
DY8307-05
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Human Hepcidin ELISA Standard Curve
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Product Details
Citations (10)
FAQs
Supplemental Products
Reviews (2)

Human Hepcidin DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Assay Range
3.1 - 200 pg/mL
Sufficient Materials
For five 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and Recombinant . The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required


PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Scientific Data

Human Hepcidin ELISA Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Citations for Human Hepcidin DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Haemoglobin diagnostic cut-offs for anaemia in Indian women of reproductive age
    Authors: Ghosh, S;Palika, R;Dasi, T;Varshney, RK;Parasannanavar, DJ;Sen Gupta, S;Chitikineni, A;Banjara, SK;Pullakhandam, R;Thomas, T;Sachdev, HS;Kurpad, AV;Kulkarni, B;
    European journal of clinical nutrition
    Species: Human
    Sample Types: Serum
  2. Relationships between Dietary Patterns and Erythropoiesis-Associated Micronutrient Deficiencies (Iron, Folate, and Vitamin B12) among Pregnant Women in Taiwan
    Authors: Mayasari, NR;Bai, CH;Chao, JC;Chen, YC;Huang, YL;Wang, FF;Wiratama, BS;Chang, JS;
    Nutrients
    Species: Human
    Sample Types: Serum
  3. Hepcidin as a Diagnostic Biomarker in Anaemic Lung Cancer Patients
    Authors: K Wadowska, P B?asiak, A Rzechonek, I Bil-Lula, M ?liwi?ska-
    Cancers, 2022-12-30;15(1):.
    Species: Human
    Sample Types: Serum
  4. Glycochenodeoxycholate Affects Iron Homeostasis via Up-Regulating Hepcidin Expression
    Authors: LJ Wang, GP Zhao, XF Wang, XX Liu, YX Li, LL Qiu, XY Wang, FZ Ren
    Nutrients, 2022-08-02;14(15):.
    Species: Human, Mouse
    Sample Types: Serum
  5. Efficacy of iron-folic acid treatment for reducing anemia prevalence and improving iron status in women of reproductive age: A one-year longitudinal study
    Authors: R Palika, T Dasi, S Ghosh, R Peter, DJ Parasannan, AS Pradhan, AV Kurpad, HS Sachdev, B Kulkarni, R Pullakhand
    Clinical Nutrition ESPEN, 2022-03-23;49(0):390-397.
    Species: Human
    Sample Types: Plasma
  6. Associations of Food and Nutrient Intake with Serum Hepcidin and the Risk of Gestational Iron-Deficiency Anemia among Pregnant Women: A Population-Based Study
    Authors: NR Mayasari, CH Bai, TY Hu, JC Chao, YC Chen, YL Huang, FF Wang, AA Tinkov, AV Skalny, JS Chang
    Nutrients, 2021-10-03;13(10):.
    Species: Human
    Sample Types: Serum
  7. Interferon-gamma promotes iron export in human macrophages to limit intracellular bacterial replication
    Authors: R Abreu, L Essler, P Giri, F Quinn
    PLoS ONE, 2020-12-08;15(12):e0240949.
    Species: Human
    Sample Types: Cell Culture Supernates
  8. Altered iron metabolism in cystic fibrosis macrophages: the impact of CFTR modulators and implications for Pseudomonas aeruginosa survival
    Authors: HF Hazlett, TH Hampton, DS Aridgides, DA Armstrong, JA Dessaint, DL Mellinger, AB Nymon, A Ashare
    Sci Rep, 2020-07-02;10(1):10935.
    Species: Human
    Sample Types: Cell Culture Supernates
  9. Plasma hepcidin is associated with future risk of venous thromboembolism.
    Authors: Ellingsen T, Lappegard J, Ueland T, Aukrust P, Braekkan S, Hansen J
    Blood Adv, 2018-06-12;2(11):1191-1197.
    Species: Human
    Sample Types: Plasma
  10. Heparin inhibits intracellular Mycobacterium tuberculosis bacterial replication by reducing iron levels in human macrophages
    Authors: R Abreu, L Essler, A Loy, F Quinn, P Giri
    Sci Rep, 2018-05-08;8(1):7296.
    Species: Human
    Sample Types: Cell Culture Supernates

FAQs

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Reviews for Human Hepcidin DuoSet ELISA

Average Rating: 4.5 (Based on 2 Reviews)

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Human Hepcidin DuoSet ELISA
By Anonymous on 11/07/2022
Sample Tested: Serum,Urine

Human Hepcidin DuoSet ELISA
By Prakasha Kempaiah on 06/03/2016
Sample Tested: Serum