Detection of Human Hexim 1 by Western Blot.
Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, Jurkat human acute T cell leukemia cell line, and Hep3B human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Hexim 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8106) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Hexim 1 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Hexim 1 in Human Liver.
Hexim 1 was detected in immersion fixed paraffin-embedded sections of human liver using Sheep Anti-Human Hexim 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8106) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Background: Hexim 1
Hexim 1, also known as CLP1, EDG1, HIS1, and MAQ1, is an approximately 60 kDa transcriptional regulator that binds double stranded RNA and inhibits RNA polymerase II. Hexim 1 plays an important role in many processes including satellite muscle cell regeneration, cardiovascular development, and HIV-1 replication. It can form homodimers and heterodimers with Hexim 2, and its activity is regulated by phosphoylation at Ser158. Hexim 1 inhibits the kinase activity of the elongation factor P-TEFb. This inhibition can be relieved by Hexim 1 interaction with Nucleophosphmin or by cellular exposure to hexamtehylene bisacetamide. Hexim 1 inhibits the transactivation function of the glucocorticoid, estrogen, and androgen receptors. It can inhibit the cellular response to hypoxia and promote cancer progression or, alternatively, can inhibit VEGF mediated angiogenesis and suppress metastasis. Within aa 213-359, human Hexim 1 shares 95% and 94% aa sequence identity with mouse and rat Hexim 1, respectively.
Hexamethylene Bis-Acetamide Inducible 1
Entrez Gene IDs:
10614 (Human); 192231 (Mouse); 498008 (Rat)
Cardiac lineage protein 1; CLP1; CLP-1; CLP1hexamethylene-bis-acetamide-inducible transcript 1; EDG1HMBA-inducible; Estrogen down-regulated gene 1 protein; hexamethylene bis-acetamide inducible 1; Hexamethylene bis-acetamide-inducible protein 1; hexamthylene bis-acetamide inducible 1; Hexim 1; HEXIM1; HIS1; HIS1FLJ13562; HMBA-inducible; MAQ1; MAQ1hexamethylene bisacetamide-inducible protein; menage a quatre 1; Menage a quatre protein 1; protein HEXIM1
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