Human HHEX Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human HHEX in direct ELISAs.
  • Source
    Monoclonal Mouse IgG1 Clone # 906705
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human HHEX 
    Thr111-Gly270
    Accession # Q03014
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
  • Simple Western
    20 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human HHEX by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human HHEX Monoclonal Antibody (Catalog # MAB8377) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HHEX at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human HHEX by Simple WesternTM. Simple Western lane view shows lysates of K562 human chronic myelogenous leukemia cell line, loaded at 0.5 mg/mL. A specific band was detected for HHEX at approximately 47 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human HHEX Monoclonal Antibody (Catalog # MAB8377). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HHEX
Hematopoietically-expressed homeobox protein (HHEX), also known as HEX, PRH and PRHX, is a 35-40 kDa member of the Homeobox family of transcription factors.  Family members are distinguished by an evolutionarily conserved DNA-binding homeodomain of 60 amino acids (aa), which for HHEX spans aa 137-196.  Human HHEX was initially isolated from hematopoietic tissue, and is present in several hematopoietic progenitors, where its expression is down-regulated during terminal cell differentiation.  HHEX is also expressed in the anterior visceral endoderm during early mouse development, and in some adult tissues of endodermal origin, including liver, lung and thyroid.  HHEX knockout in mice is embryonic lethal, with impaired forebrain, liver and thyroid development.  Human HHEX is 270 aa in length, and over aa 111-270, shares 93% and 95% identity with mouse and rat HHEX, respectively.
  • Long Name:
    Hematopoietically Expressed Homeobox
  • Entrez Gene IDs:
    3087 (Human); 15242 (Mouse); 79237 (Rat)
  • Alternate Names:
    hematopoietically expressed homeobox; Hex; HEXHMPH; HHEX; HMPH; Homeobox protein HEX; Homeobox protein PRH; HOX11L-PEN; PRH; PRHhematopoietically-expressed homeobox protein HHEX; PRHX; PRHXhomeobox, hematopoietically expressed; proline-rich homeodomain-containing transcription factor
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