High in Normal-1 (HIN-1), also known as Secretoglobulin 3A1 (SCGB3A1), PnSP-2, LuLeu-2, and UGRP-2 is an 8 kDa member of the Secretoglobulin family of molecules. It is synthesized as a 104 aa precursor that contains a 21 aa signal sequence and an 83 aa mature region. There are no potential N-linked glycosylation sites. Based on other members of the family, HIN-1 is likely to exist as a disulfide-linked homodimer. The molecule is found in mammary and upper airway epithelium. Mature mouse HIN-1 shares 60% aa sequence identity with mature human HIN-1.
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 410221
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Product Specifications
Immunogen
E. coli-derived recombinant human HIN‑1/SCGB3A1
Phe21-Gly104
Accession # Q96QR1
Phe21-Gly104
Accession # Q96QR1
Specificity
Detects human HIN‑1/SCGB3A1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant mouse HIN-1 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications for Human HIN‑1/SCGB3A1 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Human HIN‑1/SCGB3A1 under non-reducing conditions only
Sample: Recombinant Human HIN‑1/SCGB3A1 under non-reducing conditions only
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: HIN-1/SCGB3A1
Long Name
High in Normal 1
Alternate Names
HIN1, PnSP-2, SCGB3A1, UGRP2
Gene Symbol
SCGB3A1
UniProt
Additional HIN-1/SCGB3A1 Products
Product Documents for Human HIN‑1/SCGB3A1 Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human HIN‑1/SCGB3A1 Antibody
For research use only
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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