Human ICAM-1/CD54 Antibody

(32 citations)
(1 Review)
  
  • Species Reactivity
    Human
  • Specificity
    This antibody was selected for its ability to stain human ICAM-1/CD54 transfected COS cells. It does not stain COS cells transfected with human E-Selectin, VCAM-1, or PECAM-1.
  • Source
    Monoclonal Mouse IgG1 Clone # BBIG-I1 (11C81)
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Activated HUVEC human umbilical vein endothelial cells
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human ICAM‑1/CD54 Fc Chimera (Catalog # 720-IC) under non-reducing conditions only
  • Immunoprecipitation
    Davies, M.E., et al. (1991) Connect. Tissue Res. 26:207.
  • Adhesion Blockade
    The adhesion of HSB2 human peripheral blood acute lymphoblastic leukemia cells (5 x 104 cells/well) to immobilized Recombinant Human ICAM-1/CD54 Fc Chimera (Catalog # 720-IC,12.5 µg/mL, 100 µL/well) was maximally inhibited (80-100%) by 10 µg/mL of the antibody.
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
ICAM‑1/CD54 in HUVEC Cells. ICAM‑1/CD54 was detected in immersion fixed human umbilical vein endothelial cells (HUVECs) using Mouse Anti-Human
ICAM‑1/CD54 Monoclonal Antibody (Catalog # BBA3) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ICAM-1/CD54
ICAM-1 is a member of the immunoglobulin superfamily whose expression is upregulated on leukocytes, epithelial cells and resting endothelial cells in response to inflammatory signals. ICAM-1 binds the leukocyte integrins LFA-1 and Mac-1.
  • Long Name:
    Intercellular Adhesion Molecule 1
  • Entrez Gene IDs:
    3383 (Human); 15894 (Mouse); 25464 (Rat)
  • Alternate Names:
    BB2; CD54 antigen; CD54; cell surface glycoprotein P3.58; human rhinovirus receptor; ICAM1; ICAM-1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; intercellular adhesion molecule 1; Major group rhinovirus receptor; P3.58
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

32 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Endothelial CD2AP Binds the Receptor ICAM-1 To Control Mechanosignaling, Leukocyte Adhesion, and the Route of Leukocyte Diapedesis In Vitro
    Authors: A Schaefer, TJ van Duijn, J Majolee, K Burridge, PL Hordijk
    J. Immunol., 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Functional Assay
  2. A possible role for neutrophils in allergic rhinitis revealed after cellular subclassification
    Authors: J Arebro, S Ekstedt, E Hjalmarsso, O Winqvist, S Kumlien Ge, LO Cardell
    Sci Rep, 2017;7(0):43568.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. C6-ceramide nanoliposome suppresses tumor metastasis by eliciting PI3K and PKCzeta tumor-suppressive activities and regulating integrin affinity modulation.
    Authors: Zhang P, Fu C, Hu Y, Dong C, Song Y, Song E
    Sci Rep, 2015;5(0):9275.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  4. Role of cortactin homolog HS1 in transendothelial migration of natural killer cells.
    Authors: Mukherjee S, Kim J, Mooren O, Shahan S, Cohan M, Cooper J
    PLoS ONE, 2015;10(2):e0118153.
    Species: Human
    Sample Type: Whole Cells
    Application: IF
  5. Uveal melanoma cells utilize a novel route for transendothelial migration.
    Authors: Onken, Michael, Li, Jinmei, Cooper, John A
    PLoS ONE, 2014;9(12):e115472.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  6. Interaction of mesenchymal stem cells with fibroblast-like synoviocytes via cadherin-11 promotes angiogenesis by enhanced secretion of placental growth factor.
    Authors: Park, Su-Jung, Kim, Ki-Jo, Kim, Wan-Uk, Cho, Chul-Soo
    J Immunol, 2014;192(7):3003-10.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  7. Inflammation converts human mesoangioblasts into targets of alloreactive immune responses: implications for allogeneic cell therapy of DMD.
    Authors: Noviello M, Tedesco F, Bondanza A, Tonlorenzi R, Rosaria Carbone M, Gerli M, Marktel S, Napolitano S, Cicalese M, Ciceri F, Peretti G, Cossu G, Bonini C
    Mol Ther, 2014;22(7):1342-52.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  8. A composite model of the human postcapillary venule for investigation of microvascular leukocyte recruitment.
    Authors: Lauridsen H, Pober J, Gonzalez A
    FASEB J, 2014;28(3):1166-80.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  9. Tumor-associated fibroblasts isolated from colorectal cancer tissues exhibit increased ICAM-1 expression and affinity for monocytes.
    Authors: Schellerer V, Langheinrich M, Hohenberger W, Croner R, Merkel S, Rau T, Sturzl M, Naschberger E
    Oncol Rep, 2014;31(1):255-61.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC Fresh
  10. Phenolic acid composition, antiatherogenic and anticancer potential of honeys derived from various regions in Greece.
    Authors: Spilioti E, Jaakkola M, Tolonen T, Lipponen M, Virtanen V, Chinou I, Kassi E, Karabournioti S, Moutsatsou P
    PLoS ONE, 2014;9(4):e94860.
    Species: Human
    Sample Type: Whole Cells
    Application: ELISA Capture
  11. West Nile virus-induced cell adhesion molecules on human brain microvascular endothelial cells regulate leukocyte adhesion and modulate permeability of the in vitro blood-brain barrier model.
    Authors: Roe, Kelsey, Orillo, Beverly, Verma, Saguna
    PLoS ONE, 2014;9(7):e102598.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  12. Endothelial cells use dynamic actin to facilitate lymphocyte transendothelial migration and maintain the monolayer barrier.
    Authors: Mooren O, Li J, Nawas J, Cooper J
    Mol Biol Cell, 2014;25(25):4115-29.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  13. Enzyme-free passage of human pluripotent stem cells by controlling divalent cations.
    Authors: Ohnuma, Kiyoshi, Fujiki, Ayaka, Yanagihara, Kana, Tachikawa, Saoko, Hayashi, Yohei, Ito, Yuzuru, Onuma, Yasuko, Chan, Techuan, Michiue, Tatsuo, Furue, Miho K, Asashima, Makoto
    Sci Rep, 2014;4(0):4646.
    Species: Human
    Sample Type: Whole Cells
    Application: IHC
  14. Zoledronate attenuates angiotensin II-induced abdominal aortic aneurysm through inactivation of Rho/ROCK-dependent JNK and NF-kappaB pathway.
    Authors: Tsai S, Huang P, Peng Y, Chang W, Tsai H, Leu H, Chen J, Lin S
    Cardiovasc Res, 2013;100(3):501-10.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  15. GroEL1, a heat shock protein 60 of Chlamydia pneumoniae, impairs neovascularization by decreasing endothelial progenitor cell function.
    Authors: Lin, Yi-Wen, Huang, Chun-Yao, Chen, Yung-Hsi, Shih, Chun-Min, Tsao, Nai-Wen, Lin, Cheng-Ye, Chang, Nen-Chun, Tsai, Chien-Su, Tsai, Hsiao-Ya, Tsai, Jui-Chi, Huang, Po-Hsun, Li, Chi-Yuan, Lin, Feng-Yen
    PLoS ONE, 2013;8(12):e84731.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  16. ERK5 protein promotes, whereas MEK1 protein differentially regulates, the Toll-like receptor 2 protein-dependent activation of human endothelial cells and monocytes.
    Authors: Wilhelmsen K, Mesa K, Lucero J, Xu F, Hellman J
    J Biol Chem, 2012;287(32):26478-94.
    Species: Human
    Sample Type: Whole Cells
    Application: Cell-based ELISA
  17. Macrophages induce differentiation of plasma cells through CXCL10/IP-10.
    J. Exp. Med., 2012;209(10):1813-23.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  18. Endothelial cell substrate stiffness influences neutrophil transmigration via myosin light chain kinase-dependent cell contraction.
    Authors: Stroka KM, Aranda-Espinoza H
    Blood, 2011;118(6):1632-40.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  19. IL-17 amplifies human contact hypersensitivity by licensing hapten nonspecific Th1 cells to kill autologous keratinocytes.
    Authors: Pennino D, Eyerich K, Scarponi C, Carbone T, Eyerich S, Nasorri F, Garcovich S, Traidl-Hoffmann C, Albanesi C, Cavani A
    J. Immunol., 2010;184(9):4880-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  20. Adhesion of human haematopoietic (CD34+) stem cells to human liver compartments is integrin and CD44 dependent and modulated by CXCR3 and CXCR4.
    Authors: Crosby HA, Lalor PF, Ross E, Newsome PN, Adams DH
    J. Hepatol., 2009;51(4):734-49.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  21. SPARC is a VCAM-1 counter-ligand that mediates leukocyte transmigration.
    Authors: Kelly KA, Allport JR, Yu AM, Sinh S, Sage EH, Gerszten RE, Weissleder R
    J. Leukoc. Biol., 2007;81(3):748-56.
    Species: Human
    Sample Type: Whole Cells
    Application: Functional Assay
  22. Cannabidiol attenuates high glucose-induced endothelial cell inflammatory response and barrier disruption.
    Authors: Rajesh M, Mukhopadhyay P, Batkai S, Hasko G, Liaudet L, Drel VR, Obrosova IG, Pacher P
    Am. J. Physiol. Heart Circ. Physiol., 2007;293(1):H610-9.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  23. Glucocorticoid-induced tumour necrosis factor receptor-related protein-mediated macrophage stimulation may induce cellular adhesion and cytokine expression in rheumatoid arthritis.
    Authors: Bae E, Kim WJ, Kang YM, Suk K, Koh EM, Cha HS, Ahn KS, Huh TL, Lee WH
    Clin. Exp. Immunol., 2007;148(3):410-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  24. Differential survival of leukocyte subsets mediated by synovial, bone marrow, and skin fibroblasts: site-specific versus activation-dependent survival of T cells and neutrophils.
    Authors: Filer A, Parsonage G, Smith E, Osborne C, Thomas AM, Curnow SJ, Rainger GE, Raza K, Nash GB, Lord J, Salmon M, Buckley CD
    Arthritis Rheum., 2006;54(7):2096-108.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  25. Migration inhibitory factor up-regulates vascular cell adhesion molecule-1 and intercellular adhesion molecule-1 via Src, PI3 kinase, and NFkappaB.
    Authors: Amin MA, Haas CS, Zhu K, Mansfield PJ, Kim MJ, Lackowski NP, Koch AE
    Blood, 2006;107(6):2252-61.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  26. Proteinase-activated receptor2 agonists upregulate granulocyte colony-stimulating factor, IL-8, and VCAM-1 expression in human bronchial fibroblasts.
    Authors: Ramachandran R, Morice AH, Compton SJ
    Am. J. Respir. Cell Mol. Biol., 2006;35(1):133-41.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  27. Requirement for intercellular adhesion molecule 1 and caveolae in invasion of human oral epithelial cells by Porphyromonas gingivalis.
    Authors: Tamai R, Asai Y, Ogawa T
    Infect. Immun., 2005;73(10):6290-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  28. Cell expression of MMP-1 and TIMP-1 in co-cultures of human gingival fibroblasts and monocytes: the involvement of ICAM-1.
    Authors: Domeij H, Modeer T, Quezada HC, Yucel-Lindberg T
    Biochem. Biophys. Res. Commun., 2005;338(4):1825-33.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  29. Persistent cytomegalovirus infection is associated with increased expression of TGF-beta1, PDGF-AA and ICAM-1 and arterial intimal thickening in kidney allografts.
    Authors: Helantera I, Loginov R, Koskinen P, Tornroth T, Gronhagen-Riska C, Lautenschlager I
    Nephrol. Dial. Transplant., 2005;20(4):790-6.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  30. Differential effects of orbital and laminar shear stress on endothelial cells.
    Authors: Frattini J, Kudo FA
    J. Vasc. Surg., 2005;41(5):869-80.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  31. CC chemokines and transmigration of eosinophils in the presence of vascular cell adhesion molecule 1.
    Authors: Yamamoto H, Nagata M, Sakamoto Y
    Ann. Allergy Asthma Immunol., 2005;94(2):292-300.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  32. Apical topography and modulation of ICAM-1 expression on activated endothelium.
    Authors: Almenar-Queralt A, Duperray A, Miles LA, Felez J, Altieri DC
    Am. J. Pathol., 1995;147(5):1278-88.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Expand to show all 32 Citations
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Average Rating: 5 (Based on 1 review)

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We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Immunocytochemistry/Immunofluorescence.

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Images Ratings Applications Species Reviewed By Date Details
Immunocytochemistry/Immunofluorescence Human ICAM-1/CD54 Antibody BBA3
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 ICC/IF Human Anonymous 04/26/2017
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Immunocytochemistry/Immunofluorescence Human ICAM-1/CD54 Antibody BBA3
Immunocytochemistry/Immunofluorescence: Human ICAM-1/CD54 Antibody [BBA3]

Summary

ApplicationImmunocytochemistry/Immunofluorescence
Sample TestedHUVEC human umbilical vein endothelial cells
SpeciesHuman

Other Experimental Details

Other Experimental DetailsHUVECs were stimulated with LPS (10 ng/mL)

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