Human ICAM-1/CD54 Antibody

Catalog # Availability Size / Price Qty
BBA17
Human ICAM-1/CD54 Antibody in Immunocytochemistry
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Product Details
Citations (9)
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Human ICAM-1/CD54 Antibody Summary

Species Reactivity
Human
Specificity
Detects human ICAM‑1/CD54 in direct ELISAs and Western blots. ICAM‑1/CD54 has been screened using CHO cells transfected with cDNAs for ICAM-1, VCAM-1, and E-Selectin. ICAM‑1/CD54 was shown to be only reactive with ICAM-1.
Source
Polyclonal Goat Serum
Purification
N/A
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human ICAM‑1/CD54
Extracellular domain
Formulation
Lyophilized from a 0.2 μm filtered solution in Serum.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1:1000 dilution
Recombinant Human ICAM‑1/CD54 Fc Chimera (Catalog # 720-IC)
Immunohistochemistry
1:300 dilution
Immersion fixed paraffin-embedded sections of human kidney
Immunocytochemistry
1-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunocytochemistry ICAM‑1/CD54 in A431 Human Cell Line. View Larger

ICAM‑1/CD54 in A431 Human Cell Line. ICAM-1/CD54 was detected in immersion fixed A431 human epithelial carcinoma cell line using Goat Anti-Human ICAM-1/CD54 Polyclonal Antibody (Catalog # BBA17) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute in 0.5 mL of sterile water.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ICAM-1/CD54

Intercellular Adhesion Molecule-1 (ICAM-1, CD54) binds the leukocyte integrins LFA-1 and Mac-1. ICAM-1 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be stimulated by IFN-gamma, TNF-alpha, IL-1 beta and LPS.

Soluble ICAM-1 is found in a biologically active form in serum, probably as a result of proteolytic cleavage from the cell surface, and is elevated in patients with various inflammatory syndromes such as septic shock, LAD, cancer and transplantation.

References
  1. Pigott, R. and C. Power, 1993, The Adhesion Molecule Facts Book, pp. 74. Academic Press.
Long Name
Intercellular Adhesion Molecule 1
Entrez Gene IDs
3383 (Human); 15894 (Mouse); 25464 (Rat)
Alternate Names
BB2; CD54 antigen; CD54; cell surface glycoprotein P3.58; human rhinovirus receptor; ICAM1; ICAM-1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; intercellular adhesion molecule 1; Major group rhinovirus receptor; P3.58

Product Datasheets

Citations for Human ICAM-1/CD54 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Amyloid-beta 1-40 is associated with alterations in NG2+ pericyte population ex�vivo and in�vitro
    Authors: N Schultz, K Brännström, E Byman, S Moussaud, HM Nielsen,, A Olofsson, M Wennström
    Aging Cell, 2018;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  2. Changes in CD200 and intercellular adhesion molecule-1 (ICAM-1) levels in brains of Lewy body disorder cases are associated with amounts of Alzheimer's pathology not ?-synuclein pathology.
    Authors: Douglas G Walker, Lih-Fen Lue, Tiffany M Tang, Charles H Adler, John N Caviness, Marwan N Sabbagh, Geidy E Serrano, Lucia I Sue, Thomas G Beach
    Neurobiology of Aging, 2017;0(0):1558-1497.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  3. Tumor necrosis factor disrupts claudin-5 endothelial tight junction barriers in two distinct NF-kappaB-dependent phases.
    Authors: Clark P, Kim R, Pober J, Kluger M
    PLoS ONE, 2015;10(3):e0120075.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Outer membrane vesicles derived from Escherichia coli up-regulate expression of endothelial cell adhesion molecules in vitro and in vivo.
    Authors: Kim J, Yoon Y, Lee J, Choi E, Yi N, Park K, Park J, Lotvall J, Kim Y, Gho Y
    PLoS ONE, 2013;8(3):e59276.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Inflammatory cytokines stimulate the adhesion of colon carcinoma cells to mesothelial monolayers.
    Authors: van Grevenstein WM, Hofland LJ, van Rossen ME, van Koetsveld PM, Jeekel J, van Eijck CH
    Dig. Dis. Sci., 2007;52(10):2775-83.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  6. Endothelial adhesion molecule expression is unaltered in the peripheral nerve from patients with AIDS and distal sensory polyneuropathy.
    Authors: Fenzi F, Rossi F, Rava M, Cavallaro T, Ferrari S, Rizzuto N
    J. Neuroimmunol., 2006;178(1):111-6.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  7. Requirement for intercellular adhesion molecule 1 and caveolae in invasion of human oral epithelial cells by Porphyromonas gingivalis.
    Authors: Tamai R, Asai Y, Ogawa T
    Infect. Immun., 2005;73(10):6290-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  8. TNFalpha increases the inflammatory response to vascular balloon injury without accelerating neointimal formation.
    Authors: Miller AM, McPhaden AR, Preston A, Wadsworth RM, Wainwright CL
    Atherosclerosis, 2005;179(1):51-9.
    Species: Rabbit
    Sample Types: Whole Tissue
    Applications: IHC-P
  9. E-selectin and ICAM-1 are incorporated into detergent-insoluble membrane domains following clustering in endothelial cells.
    Authors: Tilghman RW, 2019, Hoover RL
    eaav5562, 2002;525(1):83-7.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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