Human ICAM-3/CD50 Antibody

Catalog # Availability Size / Price Qty
BBA29-200
Product Details
Citations (1)
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Human ICAM-3/CD50 Antibody Summary

Species Reactivity
Human
Specificity
Detects human ICAM-3/CD50 in Western blots. Does not cross-react with recombinant human (rh) ICAM-1 or rhVCAM-1.
Source
Monoclonal Mouse IgG1 Clone # Cal 3.10
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Recombinant human ICAM-3/CD50 Fc Chimera
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human ICAM‑3/CD50 Fc Chimera (Catalog # 715-IC)
Flow Cytometry
2.5 µg/106 cells
Human peripheral blood neutrophils
Adhesion Blockade
Bossy, D. et al. (1995) Eur. J. Immunol. 25:459.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ICAM-3/CD50

Intercellular Adhesion Molecule-3 (ICAM-3), a member of the immunoglobulin superfamily, binds the leukocyte integrins LFA-1 (CD11a/CD18) and alpha d/ beta 2. ICAM-3 is expressed on leukocytes and epidermal Langerhans cells. It may play an important role in T cell stimulation by Langerhans cells. ICAM-3 has not been identified in mice.

References
  1. Hayflick, J. et al. (1998) Immunologic Res. 17:313.
Long Name
Intercellular Adhesion Molecule 3
Entrez Gene IDs
3385 (Human)
Alternate Names
CD50 antigen; CD50; CDW50CDw50; ICAM3; ICAM-3; ICAM-Rintercellular adhesion molecule-3; intercellular adhesion molecule 3

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Citation for Human ICAM-3/CD50 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Macrophage recognition of ICAM-3 on apoptotic leukocytes.
    Authors: Moffatt OD, Devitt A, Bell ED
    J. Immunol., 1999-06-01;162(11):6800-10.
    Species: Human
    Sample Types: Recombinant Protein, Whole Cells
    Applications: Adhesion Assay, Direct ELISA

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