Human ICAM-5 Biotinylated Antibody

Catalog #: BAF1950 Datasheet / COA / SDS

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BAF1950

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ICAM-5 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human ICAM-5 in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant mouse ICAM-5 is observed and less than 2% cross‑reactivity with recombinant human (rh) ICAM-1, rhICAM-2, and rhICAM-3 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human ICAM-5
Ala28-Glu570
Accession # Q9UMF0

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human ICAM‑5 Fc Chimera (Catalog # 1950-M5)

Human ICAM-5 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.1-0.4 µg/mL

Use in combination with:

Capture Reagent: Human ICAM‑5 Antibody (Catalog # MAB1950)

Standard: Recombinant Human ICAM-5 Fc Chimera Protein, CF (Catalog # 1950-M5)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA Detection (Matched Antibody Pair)

Human ICAM-5 Antibody in ELISA Standard Curve.

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Human ICAM‑5 ELISA Standard Curve. Recombinant Human ICAM-5 protein (Catalog # 1950-M5) was serially diluted 2-fold and captured by Mouse Anti-Human ICAM-5 Monoclonal Antibody (Catalog # MAB1950) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human ICAM-5 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1950) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ICAM-5

Intercellular adhesion molecule-5 (ICAM-5), also known as telencephalin, is a cell surface glycoprotein belonging to the immunoglobulin superfamily. Human ICAM-5 consists of an 832 amino acid (aa) extracellular domain containing 9 immunoglobulin (Ig) domains and 15 N-glycosylation sites, a 28 aa transmembrane domain, and a 64 aa cytoplasmic domain. ICAM-5 shares 38‑55% aa identity with other ICAMs, being most closely related to ICAM-1 (50% identity) and ICAM-3 (55% identity) (1). Human and mouse ICAM-5 share 85% aa identity. The tissue distribution of ICAM-5 is unique among ICAMs, being expressed only in telencephalic regions of the central nervous system (2). Like other ICAMs, ICAM-5 binds to the leukocyte integrin LFA-1 (CD11a/CD18) (3). Binding of ICAM-5 to LFA-1 is dependent on the first amino terminal Ig domain of ICAM-5 (4). ICAM-5 also displays homophilic binding, with the amino terminal Ig domain binding to Ig domains 4‑5. Homophilic binding of ICAM-5 is dependent of ICAM-5 being in a monomeric form. The monomeric form of ICAM-5 is found during dendritogenesis in developing brain, whereas a high molecular weight complex is found in mature neurons (5).

References

Mizuno, T. et al. (1997) J. Biol. Chem. 272:1156.
Yoshihara, Y. et al. (1994) Neuron 12:541.
Tain, L. et al. (1997) J. Immunol. 158:928.
Tain, L. et al. (2000) Eur. J. Immunol. 30:810.
Tain, L. et al. (2000) J. Immunol. 150:243.

Long Name

Intercellular Adhesion Molecule 5

Entrez Gene IDs

7087 (Human); 15898 (Mouse)

Alternate Names

ICAM5; ICAM-5; intercellular adhesion molecule 5; intercellular adhesion molecule 5, telencephalin; Telencephalin; TLCNTelencephalin; TLNtelencephalin