Human ICAM-5 Antibody

Catalog # Availability Size / Price Qty
AF1950
AF1950-SP
Human ICAM-5 Antibody in Western Blot
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Product Details
Citations (1)
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Human ICAM-5 Antibody Summary

Species Reactivity
Human
Specificity
Detects human ICAM-5 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant mouse ICAM-5 is observed and less than 2% cross-reactivity with recombinant human (rh) ICAM-1, rhICAM-2 and rhICAM-3 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human ICAM-5
Ala28-Glu570
Accession # Q9UMF0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
Simple Western
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human ICAM‑5 by Western Blot. View Larger

Detection of Human ICAM‑5 by Western Blot. Western blot shows lysates of human brain (motor cortex) tissue. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human ICAM‑5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1950) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for ICAM‑5 at approximately 115 and 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection  of Human ICAM‑5 by Simple WesternTM. View Larger

Detection of Human ICAM‑5 by Simple WesternTM. Simple Western lane view shows lysates of human brain (motor cortex) tissue, loaded at 0.2 mg/mL. Specific bands were detected for ICAM‑5 at approximately 146 and 180 kDa (as indicated) using 1 µg/mL of Goat Anti-Human ICAM‑5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1950) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ICAM-5

Intercellular adhesion molecule-5 (ICAM-5), also known as telencephalin, is a cell surface glycoprotein belonging to the immunoglobulin superfamily. Human ICAM-5 consists of an 832 amino acid (aa) extracellular domain containing 9 immunoglobulin (Ig) domains and 15 N-glycosylation sites, a 28 aa transmembrane domain, and a 64 aa cytoplasmic domain. ICAM-5 shares 38‑55% aa identity with other ICAMs, being most closely related to ICAM-1 (50% identity) and ICAM-3 (55% identity) (1). Human and mouse ICAM-5 share 85% aa identity. The tissue distribution of ICAM-5 is unique among ICAMs, being expressed only in telencephalic regions of the central nervous system (2). Like other ICAMs, ICAM-5 binds to the leukocyte integrin LFA-1 (CD11a/CD18) (3). Binding of ICAM-5 to LFA-1 is dependent on the first amino terminal Ig domain of ICAM-5 (4). ICAM-5 also displays homophilic binding, with the amino terminal Ig domain binding to Ig domains 4‑5. Homophilic binding of ICAM-5 is dependent of ICAM-5 being in a monomeric form. The monomeric form of ICAM-5 is found during dendritogenesis in developing brain, whereas a high molecular weight complex is found in mature neurons (5).

References
  1. Mizuno, T. et al. (1997) J. Biol. Chem. 272:1156.
  2. Yoshihara, Y. et al. (1994) Neuron 12:541.
  3. Tain, L. et al. (1997) J. Immunol. 158:928.
  4. Tain, L. et al. (2000) Eur. J. Immunol. 30:810.
  5. Tain, L. et al. (2000) J. Immunol. 150:243.
Long Name
Intercellular Adhesion Molecule 5
Entrez Gene IDs
7087 (Human); 15898 (Mouse)
Alternate Names
ICAM5; ICAM-5; intercellular adhesion molecule 5; intercellular adhesion molecule 5, telencephalin; Telencephalin; TLCNTelencephalin; TLNtelencephalin

Product Datasheets

Citation for Human ICAM-5 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. ICAM-5/Telencephalin Is a Functional Entry Receptor for Enterovirus D68
    Authors: Wei Wei
    Cell Host Microbe, 2016;20(5):631-641.
    Applications: Western Blot

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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