Human ICAM-5 Antibody

Catalog # Availability Size / Price Qty
MAB1950
MAB1950-SP
Human ICAM‑5 ELISA Standard Curve.
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Human ICAM-5 Antibody Summary

Species Reactivity
Human
Specificity
Detects human ICAM-5 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) CD31, rhCEACAM-1, recombinant mouse (rm) DCC, rhICAM-1, -2, -3, rmICAM-5, rhMAdCAM-1, or rmVCAM-1 is observed.
Source
Monoclonal Mouse IgG2A Clone # 283607
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human ICAM-5
Ala28-Glu570
Accession # Q9UMF0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample

Human ICAM-5 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human ICAM‑5 Biotinylated Antibody (Catalog # BAF1950)

Standard: Recombinant Human ICAM-5 Fc Chimera Protein, CF (Catalog # 1950-M5)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA Capture (Matched Antibody Pair) Human ICAM-5 Antibody in ELISA Standard Curve. View Larger

Human ICAM‑5 ELISA Standard Curve. Recombinant Human ICAM-5 protein (Catalog # 1950-M5) was serially diluted 2-fold and captured by Mouse Anti-Human ICAM-5 Monoclonal Antibody (Catalog # MAB1950) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human ICAM-5 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1950) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ICAM-5

Intercellular adhesion molecule-5 (ICAM-5), also known as telencephalin, is a cell surface glycoprotein belonging to the immunoglobulin superfamily. Human ICAM-5 consists of an 832 amino acid (aa) extracellular domain containing 9 immunoglobulin (Ig) domains and 15 N‑glycosylation sites, a 28 aa transmembrane domain, and a 64 aa cytoplasmic domain. ICAM-5 shares 38‑55% aa identity with other ICAMs, being most closely related to ICAM-1 (50% identity) and ICAM-3 (55% identity) (1). Human and mouse ICAM-5 share 85% aa identity. The tissue distribution of ICAM-5 is unique among ICAMs, being expressed only in telencephalic regions of the central nervous system (2). Like other ICAMs, ICAM-5 binds to the leukocyte integrin LFA-1 (CD11a/CD18) (3). Binding of ICAM-5 to LFA-1 is dependent on the first amino terminal Ig domain of ICAM-5 (4). ICAM-5 also displays homophilic binding, with the amino terminal Ig domain binding to Ig domains 4‑5. Homophilic binding of ICAM-5 is dependent of ICAM-5 being in a monomeric form. The monomeric form of ICAM-5 is found during dendritogenesis in developing brain, whereas a high molecular weight complex is found in mature neurons (5).

References
  1. Mizuno, T. et al. (1997) J. Biol. Chem. 272:1156.
  2. Yoshihara, Y. et al. (1994) Neuron 12:541.
  3. Tain, L. et al. (1997) J. Immunol. 158:928.
  4. Tain, L. et al. (2000) Eur. J. Immunol. 30:810.
  5. Tain, L. et al. (2000) J. Immunol. 150:243.
Long Name
Intercellular Adhesion Molecule 5
Entrez Gene IDs
7087 (Human); 15898 (Mouse)
Alternate Names
ICAM5; ICAM-5; intercellular adhesion molecule 5; intercellular adhesion molecule 5, telencephalin; Telencephalin; TLCNTelencephalin; TLNtelencephalin

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Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

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