Detection of Human IFITM2/IFITM3/Fragilis by Western Blot.
Western blot shows lysates of human brain tissue and human spleen tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human IFITM2/3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4834) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Specific bands were detected for IFITM2 and IFITM3 at approximately 15 and 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
IFITM3 (IFN-inducible tranemembrane protein 3; also IFN-inducible protein 1-8U and fragilis) is a 14.6 kDa (predicted) member of the CD225 family of proteins. It is expressed on primordial germ cells and select tumor types, and appears to regulate cell migration. Human IFITM3 is a 133 amino acid (aa), two-transmembrene protein that contains an N-terminal (aa 1-57) and a C-terminal (aa 108-128) extracellular domain. Over aa 3-57, human IFITM3 is 59% and 56% aa identical to bovine and mouse IFITM3, respectively. Over aa 3-57 human IFITM3 is 88% identical to human IFITM2.
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