hh Human IFN-gamma  R1/CD119 Antibody MAB6732-100: R&D Systems

Human IFN-gamma  R1/CD119 Antibody

(4 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human IFN‑ gamma R1/CD119 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse IFN-gamma R1, recombinant human (rh) IFN-gamma R2, or rhIL-10 R beta is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # GIR208
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Human placenta-derived IFN‑ gamma R1/CD119
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Neutralization
    Measured by its ability to neutralize IFN‑ gamma  R1/CD119-mediated inhibition of EMCV-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line. The Neutralization Dose (ND50) is typically 0.03-0.15 µg/mL in the presence of 2 ng/mL Recombinant Human IFN‑ gamma.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
IFN‑ gamma  Inhibition of EMCV-induced Cytopathy and Neutralization by Human IFN‑ gamma  R1/CD119 Antibody. Recombinant Human IFN‑ gamma (Catalog # 285-IF) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line in a dose‑dependent manner (orange line). Inhibition of EMCV activity elicited by Recombinant Human IFN‑ gamma (2 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IFN‑ gamma  R1/CD119 Mono­clonal Antibody (Catalog # MAB6732). The ND50 is typically 0.03‑0.15 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IFN-gamma R1/CD119

The high-affinity IFN-gamma  receptor complex is made up of two type I membrane proteins, IFN-gamma  R1 (IFN-gamma  R alpha ) and IFN-gamma  R2 (IFN-gamma  R beta ). Both proteins are members of the type II cytokine receptor family and share approximately 52% amino acid sequence identity. IFN-gamma  R1 is the ligand-binding subunit that is necessary and sufficient for IFN-gamma binding and receptor internalization. IFN-gamma  R2 is required for IFN-gamma signaling but does not bind IFN-gamma by itself. Human IFN-gamma  R1 cDNA encodes a 499 amino acid (aa) protein with a 17 aa signal peptide, a 228 aa extracellular domain, a 23 aa transmembrane domain, and a 221 aa intracellular domain. Human and mouse IFN-gamma  R1 share 52% aa identity and bind IFN-gamma in a species-specific manner. IFN-gamma  R1 is constitutively expressed in most cell types. Soluble IFN-gamma  R1 that binds IFN-gamma has been detected in biological fluids.

  • References:
    1. Bach, E.A. et al. (1997) Annu. Rev. Immunol. 15:563.
  • Long Name:
    Interferon gamma Receptor 1
  • Entrez Gene IDs:
    3459 (Human); 15979 (Mouse)
  • Alternate Names:
    AVP, type 2; CD119 antigen; CD119; FLJ45734; IFN-gamma R1; IFN-gamma receptor 1; IFNgammaR1; IFN-gamma-R1; IFNGR; IFNGR1; IFN-gR1; immune interferon receptor 1; interferon gamma receptor 1; interferon-gamma receptor alpha chain; type 2
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Species
Applications
Sample Type
  1. Bispecific T cell engager (BiTE�) antibody constructs can mediate bystander tumor cell killing
    Authors: SL Ross, M Sherman, PL McElroy, JA Lofgren, G Moody, PA Baeuerle, A Coxon, T Arvedson
    PLoS ONE, 2017;12(8):e0183390.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  2. Chronic lymphocytic leukemia cells express CD38 in response to Th1 cell-derived IFN-gamma by a T-bet-dependent mechanism.
    Authors: Burgler S, Gimeno A, Parente-Ribes A, Wang D, Os A, Devereux S, Jebsen P, Bogen B, Tjonnfjord G, Munthe L
    J Immunol, 2015;194(2):827-35.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  3. IL-22 suppresses IFN-gamma-mediated lung inflammation in asthmatic patients.
    Authors: Pennino D, Bhavsar P, Effner R, Avitabile S, Venn P, Quaranta M, Marzaioli V, Cifuentes L, Durham S, Cavani A, Eyerich K, Chung K, Schmidt-Weber C, Eyerich S
    J Allergy Clin Immunol, 2013;131(2):562-70.
    Species: Human
    Sample Type: Whole Cells
    Application: Blocking
  4. A cytokine-to-chemokine axis between T lymphocytes and keratinocytes can favor Th1 cell accumulation in chronic inflammatory skin diseases.
    Authors: Albanesi C, Scarponi C, Sebastiani S, Cavani A, Federici M, Sozzani S, Girolomoni G
    J. Leukoc. Biol., 2001;70(4):617-23.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
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