Human IGDCC3 Antibody Summary
Accession # Q81VU1
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
IGDCC3 in HepG2 Human Cell Line. IGDCC3 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human IGDCC3 Monoclonal Antibody (Catalog # MAB8559) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human IGDCC3 by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line either mock-transfected or transfected with human IGDCC3. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human IGDCC3 Monoclonal Antibody (Catalog # MAB8559) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IGDCC3 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of IGDCC3 in HEK293 Human Cell Line Transfected with Human IGDCC3 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human IGDCC3 and eGFP was stained with either (A) Mouse Anti-Human IGDCC3 Monoclonal Antibody (Catalog # MAB8559) or (B) Mouse IgG1Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
IGDCC3 (Immunoglobulin superfamily DCC subclass member 3), also known as Punc, is 814 aa single-pass type membrane protein and shares 84% aa identity with mouse IGDCC3. It is a member of the neural cell adhesion molecule family and contains two fibronectin type III repeats and four immunoglobulin-like domains. IDGCC3 is high expressed in the brain and has been suggested to play a role in motor coordination. It has also been suggested to play a role in breast cancer.
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