Human IGDCC3 Antibody

Catalog # Availability Size / Price Qty
MAB8559
MAB8559-SP
Detection of Human IGDCC3 by Western Blot.
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Human IGDCC3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IGDCC3 in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 920038
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human embryonic kidney cell line HEK293-derived recombinant human IGDCC3
Met1-Gly640
Accession # Q81VU1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human IGDCC3 by Western Blot. View Larger

Detection of Human IGDCC3 by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line either mock-transfected or transfected with human IGDCC3. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human IGDCC3 Monoclonal Antibody (Catalog # MAB8559) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IGDCC3 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of IGDCC3 in HEK293 Human Cell Line Transfected with Human IGDCC3 and eGFP by Flow Cytometry. View Larger

Detection of IGDCC3 in HEK293 Human Cell Line Transfected with Human IGDCC3 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human IGDCC3 and eGFP was stained with either (A) Mouse Anti-Human IGDCC3 Monoclonal Antibody (Catalog # MAB8559) or (B) Mouse IgG1 Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Immunocytochemistry IGDCC3 in HepG2 Human Cell Line. View Larger

IGDCC3 in HepG2 Human Cell Line. IGDCC3 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human IGDCC3 Monoclonal Antibody (Catalog # MAB8559) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGDCC3

IGDCC3 (Immunoglobulin superfamily DCC subclass member 3), also known as Punc, is 814 aa single-pass type membrane protein and shares 84% aa identity with mouse IGDCC3.  It is a member of the neural cell adhesion molecule family and contains two fibronectin type III repeats and four immunoglobulin-like domains.  IDGCC3 is high expressed in the brain and has been suggested to play a role in motor coordination.  It has also been suggested to play a role in breast cancer. 

Long Name
Immunoglobulin Superfamily, DCC Subclass, Member 3
Entrez Gene IDs
9543 (Human); 19289 (Mouse)
Alternate Names
HsT18880; IGDCC3; immunoglobulin superfamily DCC subclass member 3; immunoglobulin superfamily, DCC subclass, member 3; PUNC; Putative neuronal cell adhesion moleculePUNCHsT18880

Product Datasheets

FAQs

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Cell and Tissue Staining Kits

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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