Human IGF-II/IGF2 Antibody
Human IGF-II/IGF2 Antibody Summary
Accession # P01344.1
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Cell Proliferation Induced by IGF-II/IGF2 and Neutralization by Human IGF-II/IGF2 Antibody. Recombinant Human IGF-II/IGF2 (Catalog # 292-G2) stimulates proliferation in the MCF-7 human breast cancer cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human IGF-II/IGF2 (14 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IGF-II/IGF2 Monoclonal Antibody (Catalog # MAB292). The ND50 is typically 2-12 µg/mL.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Insulin-like growth factor I (also known as somatomedin C and somatomedin A) and insulin-like growth factor II (multiplication stimulating activity or MSA) belong to the family of insulin-like growth factors that are structurally homologous to proinsulin. Mature IGF-I and IGF-II share approximately 70% sequence identity. Both IGF-I and IGF-II are expressed in many tissues and cell types and may have autocrine, paracrine and endocrine functions. Mature IGF-I and IGF-II are highly conserved (100% identity between human, bovine, and porcine proteins) and exhibit cross-species activity.
IGF-II is a potent mitogenic growth factor. However, unlike IGF-I which has important postnatal roles, the growth-promoting function of IGF-II is limited to embryonic development.
Two specific cell surface receptors that bind IGF-I and IGF-II have been identified. The type I IGF receptor that participates in IGF signaling is structurally related to the insulin receptor. It is a disulfide-linked heterotetrameric transmembrane glycoprotein with an intracellular tyrosine kinase domain. Type I IGF receptor binds IGF-I with higher affinity than IGF-II. The type II IGF receptor which binds IGF-II with much higher affinity than IGF-I is also the cation-independent mannose 6-phosphate receptor. At the present time, it is not known if the type II IGF receptor participates in the IGF signaling pathway. An additional unknown receptor which mediates IGF‑II signaling has also been proposed. Circulating IGFs exist in complexes bound to IGF binding proteins. Currently, at least six high affinity binding proteins have been identified.
Citations for Human IGF-II/IGF2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 4
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Immune targeting of autocrine IGF2 hampers rhabdomyosarcoma growth and metastasis
Authors: C De Giovann, P Nanni, L Landuzzi, ML Ianzano, G Nicoletti, S Croci, A Palladini, PL Lollini
BMC Cancer, 2019-02-07;19(1):126.
Sample Types: Serum
Applications: ELISA Standard
Matrix metalloproteinase-1 facilitates MSC migration via cleavage of IGF-2/IGFBP2 complex
Authors: SP Guan, ATL Lam, JP Newman, KLM Chua, CYL Kok, ST Chong, MLK Chua, PYP Lam
FEBS Open Bio, 2017-11-23;8(1):15-26.
Sample Types: Whole Cells
Co-targeting the IGF system and HIF-1 inhibits migration and invasion by (triple-negative) breast cancer cells.
Authors: Mancini M, Gariboldi M, Taiana E, Bonzi M, Craparotta I, Pagin M, Monti E
Br J Cancer, 2014-05-22;110(12):2865-73.
Sample Types: Whole Cells
Association between paternally inherited haplotypes upstream of the insulin gene and umbilical cord IGF-II levels.
Authors: Adkins RM, Fain JN, Krushkal J
Pediatr. Res., 2007-10-01;62(4):451-5.
Sample Types: Plasma
Applications: ELISA Development
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