Detection of Human IGSF3 by Western Blot.
Western blot shows lysates of human lung tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human IGSF3 Monoclonal Antibody (Catalog # MAB4788) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IGSF3 at approximately 200 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.
Detection of IGSF3 in A549 Human Cell Line by Flow Cytometry.
A549 human lung carcinoma cell line was stained with Mouse Anti‑Human IGSF3 Monoclonal Antibody (Catalog # MAB4788, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B).
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
IGSF3 (Immunoglobulin superfamily member 3; also EWI-3) is a 133 kDa (predicted) member of the EWI subfamily of the Ig-Superfamily of molecules. It is reported to be widely expressed, with concentration in placenta and lung. Human IGSF3 an 1194 amino acid (aa) type I transmembrane protein that contains a 19 aa signal sequence followed by a 1175 aa mature region (aa 20-1194). The molecule's extracellular region is 1105 aa in length (aa 20-1124), and contains eight C2-type Ig-like domains (aa 22-1097). This region also possesses at least two utilized phosphorylation sites at Thr617and Ser625, plus an EWI motif in the second Ig-like domain. IGSF3 likely exists as a disulfide-linked homodimer. There are two potential isoform variants. One contains a 20 aa insertion after Pro406, while another shows the same insert coupled to a premature truncation after Pro833. Over aa 20‑1125, human IGSF3 shares 92% aa identity with mouse IGSF3.
Immunoglobulin Superfamily, Member 3
Entrez Gene IDs:
3321 (Human); 78908 (Mouse); 295325 (Rat)
EWI-3; glu-Trp-Ile EWI motif-containing protein 3; IGSF3; immunoglobulin superfamily, member 3; member 3; V8
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