Human IGSF3 Antibody

Catalog # Availability Size / Price Qty
Detection of Human IGSF3 by Western Blot.
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Product Details
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Human IGSF3 Antibody Summary

Species Reactivity
Detects human IGSF3 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) IGSF4, 4B, 4C, 4D or 8 is observed.
Monoclonal Mouse IgG1 Clone # 503621
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant human IGSF3
Accession # AAI44133
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
Human lung tissue under non-reducing conditions only
Flow Cytometry
2.5 µg/106 cells
See below
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human IGSF3 antibody by Western Blot. View Larger

Detection of Human IGSF3 by Western Blot. Western blot shows lysates of human lung tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human IGSF3 Monoclonal Antibody (Catalog # MAB4788) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IGSF3 at approximately 200 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of IGSF3 antibody in A549 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of IGSF3 in A549 Human Cell Line by Flow Cytometry. A549 human lung carcinoma cell line was stained with Mouse Anti-Human IGSF3 Monoclonal Antibody (Catalog # MAB4788, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGSF3

IGSF3 (Immunoglobulin superfamily member 3; also EWI-3) is a 133 kDa (predicted) member of the EWI subfamily of the Ig-Superfamily of molecules. It is reported to be widely expressed, with concentration in placenta and lung. Human IGSF3 an 1194 amino acid (aa) type I transmembrane protein that contains a 19 aa signal sequence followed by a 1175 aa mature region (aa 20-1194). The molecule's extracellular region is 1105 aa in length (aa 20-1124), and contains eight C2-type Ig-like domains (aa 22-1097). This region also possesses at least two utilized phosphorylation sites at Thr617and Ser625, plus an EWI motif in the second Ig-like domain. IGSF3 likely exists as a disulfide-linked homodimer. There are two potential isoform variants. One contains a 20 aa insertion after Pro406, while another shows the same insert coupled to a premature truncation after Pro833. Over aa 20‑1125, human IGSF3 shares 92% aa identity with mouse IGSF3.

Long Name
Immunoglobulin Superfamily, Member 3
Entrez Gene IDs
3321 (Human); 78908 (Mouse); 295325 (Rat)
Alternate Names
EWI-3; glu-Trp-Ile EWI motif-containing protein 3; IGSF3; immunoglobulin superfamily, member 3; member 3; V8

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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