Human IL-16 Antibody (70719)

R&D Systems | Catalog # MAB316

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, ELISA Capture (Matched Antibody Pair), Simple Western

Cited:

Western Blot, Luminex Development

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 70719
View all IL-16 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human IL-16 isoform 1
Met1203-Ser1332
Accession # Q14005

Specificity

Detects human IL-16 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human IL-16 Antibody (70719)

Detection of Human IL-16 antibody by Western Blot.

Detection of Human IL‑16 by Western Blot.

Western blot shows lysate of human tonsil tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human IL-16 Monoclonal Antibody (Catalog # MAB316) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for IL-16 at approximately 45-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human IL-16 antibody by Simple WesternTM.

Detection of Human IL‑16 by Simple WesternTM.

Simple Western lane view shows lysates of human tonsil tissue, loaded at 0.2 mg/mL. A specific band was detected for IL‑16 at approximately 50 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human IL‑16 Monoclonal Antibody (Catalog # MAB316). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Human IL-16 ELISA Standard Curve

Human IL-16 ELISA Standard Curve

Recombinant Human IL‑16 (Catalog # 316-IL) was serially diluted and captured by Mouse Anti-Human IL‑16 Monoclonal Antibody (Catalog # MAB316) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑16 C-terminal Peptide Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-316-PB) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human IL-16 Antibody (70719)

Application
Recommended Usage

Simple Western

20 µg/mL
Sample: Human tonsil tissue

Western Blot

2 µg/mL
Sample: Human tonsil tissue

Human IL-16 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human IL‑16 C-terminal Peptide Biotinylated Antibody (Catalog # BAF316)
  • Standard: Recombinant Human IL-16 Protein (Catalog # 316-IL)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 4.5 using MAB316 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-16

Interleukin 16, also named lymphocyte chemoattractant factor (LCF), was originally identified as a CD8+ T-cell-derived chemoattractant for CD4+ cells. The biologically active form of IL-16 was originally proposed to be a homotetramer of 14 kDa chains containing 130 amino acid residue subunits. The complete pro-IL-16 cDNA was subsequently cloned and shown to encode a 631 amino acid residue hydrophilic protein that lacked a signal peptide. The original 130 amino acid residue polypeptide is now believed to have been derived from the C terminus of the precursor. IL-16 precursor protein has been detected in the lysates of various cells including mitogen stimulated PBMCs. The biologically active and secreted natural IL-16 is assumed to be a proteolytic cleavage product of pro-IL-16 generated by proteases present in or on activated CD8+ cells. A likely cleavage site was proposed to be at aspartate residue 510. This would yield a 121 amino acid residue protein, smaller than the 130 aa residue protein first described. The expression of IL-16 precursor mRNA has been detected in various tissues including spleen, thymus, lymph nodes, peripheral leukocytes, bone marrow and cerebellum. The gene for IL-16 precursor has been localized to chromosome 15. The biological activities ascribed to IL-16 are reported to be dependent on the cell surface expression of CD4, suggesting that IL-16 is a CD4 ligand. Besides its chemotactic properties, IL-16 has also been shown to suppress HIV-1 replication in vitro. Recombinant E. coli-derived IL-16 produced at R&D Systems is present mostly as a monomer, exhibits chemotactic activity for lymphocytes at high concentrations, lacks chemotactic activites for monocytes, and binds the extracellular domain of CD4 with low affinity.

References

  1. Cruikshank, W.W. et al. (1994) Proc. Natl. Acad. Sci. USA 91:5109.
  2. Baier, M. et al. (1997) Proc. Natl. Acad. Sci. USA 94:5273.
  3. Zhou, A. et al. (1997) Nature Medicine 3:659.
  4. Bazan, J.F. and T.J. Schall (1996) Nature 381:29.

Long Name

Interleukin 16

Alternate Names

IL16, LCF

Entrez Gene IDs

3603 (Human); 16170 (Mouse)

Gene Symbol

IL16

UniProt

Q14005

Additional IL-16 Products

Product Documents for Human IL-16 Antibody (70719)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human IL-16 Antibody (70719)

For research use only

Citations for Human IL-16 Antibody (70719)

Customer Reviews for Human IL-16 Antibody (70719) (2)

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2 Customer Ratings
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  • Human IL-16 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 08/20/2021
    Works as the capture in an ELISA.
    Human IL-16 Antibody (70719) MAB316
  • Human IL-16 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 11/16/2017
    A sandwich ELISA was made with MAB316 as the capture and biotinylated AF-316 as the detection using 316-IL as the immunoassay calibrator.
    Human IL-16 Antibody (70719) MAB316

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