Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA Capture (Matched Antibody Pair)

Cited:

ELISA Capture

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 136031
View all IL-18 BPa Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human IL-18 BPa
Thr31-Gly194 (predicted)
Accession # O95998

Specificity

Detects human IL-18 BPa in ELISAs. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant human (rh) IL-18, recombinant mouse (rm) IL-18 BPc and rmIL-18 BPd is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human IL‑18 BPa Antibody

Human IL-18 BPa Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human IL‑18 BPa Biotinylated Antibody (Catalog # BAF119)
  • Standard: Recombinant Human IL-18 BPa Fc Chimera Protein, CF (Catalog # 119-BP)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using MAB1192 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-18 BPa

Interleukin 18 binding protein (IL-18 BP) is a secreted glycoprotein, which functions as an IL-18 antagonist by binding to IL-18 and blocking its biological activity.
IL‑18 BP bears no amino acid sequence homology to the membrane-associated IL-18 and IL-1 receptor proteins. The gene for human IL-18 BP has been localized to chromosome 11q13. It encodes for at least four isoforms by alternative splicing. The IL-18 BP isoforms a and c each contain one immunoglobulin (Ig)-like C2-type domain while isoforms b and d lack a complete Ig domain. The complete Ig domain has been shown to be essential to the binding and neutralizing properties of the binding proteins. Two isoforms of mouse IL18 BP (c and d) containing the complete Ig domain have also been isolated and shown to neutralize IL-18 bioactivity. Human and mouse IL-18 BPs share approximately 61% amino acid sequence identity. Several poxviruses also encode proteins with sequence similarity to the human and mouse IL-18 BP. Viral IL-18 BPs have been shown to bind and inhibit IL-18 responses and may be involved in modulating host immune responses. The expression of IL-18 BP is markedly upregulated by IFN-gamma, suggesting that IL-18 activity is modulated by a negative feedback mechanism mediated by IL-18 BP.

References

  1. Mühl, H. et al. (2000) Biochem. Biophys. Res. Commun. 267:960.
  2. Kim, S-H. et al. (2000) Proc. Nat. Acad. Sci. USA 97:1190.
  3. Calderara, S. et al. (2001) Virology 279:22.

Long Name

Interleukin 18 Binding Protein Isoform a

Alternate Names

IL18 BPa, IL18BP

Entrez Gene IDs

10068 (Human); 84388 (Rat); 107126419 (Cynomolgus Monkey)

Gene Symbol

IL18BP

UniProt

O95998

Additional IL-18 BPa Products

Product Documents for Human IL‑18 BPa Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human IL‑18 BPa Antibody

For research use only

Related Research Areas

IL-1 Family

Citations for Human IL‑18 BPa Antibody

Customer Reviews for Human IL‑18 BPa Antibody (1)

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  • Human IL-18 BPa Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Cell culture supernatant
    Species: Human
    Verified Customer | Posted 11/09/2017
    MAB1192 was used as the capture and AF119 was used as the detection antibody for the sandwich ELISA for IL-18PB. The immunoassay standard was 119-BP. Assay had sensitivity of ~10pg/ml.
    Human IL‑18 BPa Antibody MAB1192

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