Human IL-1 beta /IL-1F2 PE-conjugated Antibody

(4 citations)   
  • Species Reactivity
  • Specificity
    Detects human IL-1 beta /IL-1F2 in Western blots. Shows less than 5% cross-reactivity with recombinant mouse (rm) IL‑1 beta and recombinant porcine IL‑1 beta and no cross-reactivity with recombinant rat (rr) IL‑1 beta, rmIL‑1 alpha, recombinant human IL‑1ra, rmIL‑1ra, or rrIL‑1 alpha.
  • Source
    Monoclonal Mouse IgG1 Clone # 8516
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human IL-1 beta /IL-1F2
    aa 117-269
    Accession # P01584
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of IL‑1 beta /IL‑1F2 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes stimulated with LPS were stained with Mouse Anti-Human CD14 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB3832F) and either (A) Mouse Anti-Human IL‑1 beta /IL‑1F2 PE‑conjugated Monoclonal Antibody (Catalog # IC201P) or (B) Mouse IgG1 Phycoerythrin Isotype Control (Catalog # IC002P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-1 beta/IL-1F2

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1 - 4). The human IL-1 beta cDNA encodes a 269 aa precursor. A 116 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5-7). The 17 kDa mature human IL-1 beta shares 96% aa sequence identity with rhesus and 67-78% with canine, cotton rat, equine, feline, mouse, porcine, and rat IL-1 beta.

  • References:
    1. Allan, S.M. et al. (2005) Nat. Rev. Immunol. 5:629.
    2. Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.
    3. Kornman, K.S. (2006) Am. J. Clin. Nutr. 83:475S.
    4. Isoda, K. and F. Ohsuzu (2006) J. Atheroscler. Thromb. 13:21.
    5. March, C.J. et al. (1985) Nature 315:641.
    6. Auron, P.E. et al. (1984) Proc. Natl. Acad. Sci. USA 81:7907.
    7. Martinon, F. and J. Tschopp (2007) Cell Death Differ. 14:10.
  • Long Name:
    Interleukin 1 beta
  • Entrez Gene IDs:
    3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)
  • Alternate Names:
    catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1B; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta
Related Research Areas

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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Sample Type
  1. Identification of IDO-positive and IDO-negative human dendritic cells after activation by various proinflammatory stimuli.
    Authors: Von Bubnoff D, Scheler M, Wilms H, Fimmers R, Bieber T
    J. Immunol., 2011;186(12):6701-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. A novel small molecule inhibitor of signal transducers and activators of transcription 3 reverses immune tolerance in malignant glioma patients.
    Authors: Hussain SF, Kong LY, Jordan J, Conrad C, Madden T, Fokt I, Priebe W, Heimberger AB
    Cancer Res., 2007;67(20):9630-6.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. A reducing microenvironment leads to the generation of FcepsilonRIhigh inflammatory dendritic epidermal cells (IDEC).
    Authors: Novak N, Kraft S, Haberstok J, Geiger E, Allam P, Bieber T
    J. Invest. Dermatol., 2002;119(4):842-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  4. Human V gamma 2V delta 2 T cells augment migration-inhibitory factor secretion and counteract the inhibitory effect of glucocorticoids on IL-1 beta and TNF-alpha production.
    Authors: Wang L, Das H, Kamath A, Li L, Bukowski JF
    J. Immunol., 2002;168(10):4889-96.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 PE-conjugated Antibody

Ctrl IC002P 28  
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Staining Reagents
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Flow Cytometry Permeabilization/Wash Buffer I (1X)

Flow FC005 4
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Flow Cytometry Fixation Buffer (1X)

Flow FC004 3
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Flow Cytometry Fixation & Permeabilization Buffer Kit I

Flow FC009
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Flow Cytometry Fixation/Permeabilization Buffer I (1X)

Flow FC007
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