Human IL‑1 beta /IL‑1F2 APC‑conjugated Antibody

Name: Human IL‑1 beta /IL‑1F2 APC‑conjugated Antibody
Brand: R&D Systems
SKU: IC201A
Price: 439 USD
Availability: InStock

R&D Systems | Catalog # IC201A

Submit a review

Citations (1)

Product Documents

View Available Conjugates

100% Guarantee

Product Specifications
Scientific Data
Applications
Spectra Viewer
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Intracellular Staining by Flow Cytometry

Cited:

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG₁ Clone # 8516

View all IL-1 beta/IL-1F2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human IL-1 beta /IL-1F2
aa 117-269
Accession # P01584

Specificity

Detects human IL-1 beta /IL-1F2 in Western blots. Shows less than 5% cross-reactivity with recombinant mouse (rm) IL‑1 beta and rpIL‑1 beta and no cross-reactivity with rrIL‑1 beta, rmIL‑1 alpha, rhIL‑1ra, rmIL‑1ra, or rrIL‑1 alpha.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human IL‑1 beta /IL‑1F2 APC‑conjugated Antibody

Detection of IL‑1 beta /IL‑1F2 in LPS-treated Peripheral Blood Mononuclear Cell Monocytes by Flow Cytometry.

Peripheral blood mononuclear cell monocytes, resting (open histogram), or LPS-treated (filled histogram) were stained with Mouse Anti-Human IL-1 beta /IL-1F2 APC-conjugated Monoclonal Antibody (Catalog # IC201A) or isotype control antibody (Catalog # IC002A). View our protocol for Staining Intracellular Molecules.

Applications for Human IL‑1 beta /IL‑1F2 APC‑conjugated Antibody

Application

Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells
Sample: Peripheral blood mononuclear cell monocytes treated with LPS, fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

Build Your Panel Now

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: IL-1 beta/IL-1F2

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2, IL1B), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. The 17 kDa molecular weight mature human IL-1 beta shares 96% aa sequence identity with rhesus and 67%-78% with canine, cotton rat, equine, feline, mouse, porcine, and rat IL-1 beta. IL-1 beta functions in a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases.