Human IL-1 beta /IL-1F2 APC-conjugated Antibody
Human IL-1 beta /IL-1F2 APC-conjugated Antibody Summary
Accession # P01584
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of IL‑1 beta /IL‑1F2 in LPS-treated Peripheral Blood Mononuclear Cell Monocytes by Flow Cytometry. Peripheral blood mononuclear cell monocytes, resting (open histogram), or LPS-treated (filled histogram) were stained with Mouse Anti-Human IL-1 beta /IL-1F2 APC-conjugated Monoclonal Antibody (Catalog # IC201A) or isotype control antibody (Catalog # IC002A). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
Background: IL-1 beta/IL-1F2
IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2, IL1B), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. The 17 kDa molecular weight mature human IL-1 beta shares 96% aa sequence identity with rhesus and 67%-78% with canine, cotton rat, equine, feline, mouse, porcine, and rat IL-1 beta. IL-1 beta functions in a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases.
Citation for Human IL-1 beta /IL-1F2 APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Immunogenicity of Novel AAV Capsids for Retinal Gene Therapy
Authors: M Gehrke, M Diedrichs-, J Bogedein, H Büning, S Michalakis, G Wildner
Sample Types: Whole Cells
Applications: Flow Cytometry
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Reviews for Human IL-1 beta /IL-1F2 APC-conjugated Antibody
There are currently no reviews for this product. Be the first to review Human IL-1 beta /IL-1F2 APC-conjugated Antibody and earn rewards!
Have you used Human IL-1 beta /IL-1F2 APC-conjugated Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image