Human IL-1 beta /IL-1F2 Fluorescein-conjugated Antibody

(4 citations)   
  • Species Reactivity
  • Specificity
    Detects human IL-1 beta /IL-1F2 in Western blots. Shows less than 5% cross-reactivity with recombinant mouse (rm) IL-1 beta /IL-1F2 and recombinant porcine IL-1 beta /IL-1F2 and no cross-reactivity with recombinant rat (rr) IL-1 beta /IL-1F2, rmIL‑1 alpha, recombinant human IL‑1ra, rmIL‑1ra, or rrIL‑1 alpha.
  • Source
    Monoclonal Mouse IgG1 Clone # 8516
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human IL-1 beta /IL-1F2
    Accession # P01584
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of IL‑1 beta /IL‑1F2 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes treated with LPS were stained with (A) Mouse Anti-Human IL‑1 beta /IL‑1F2 Fluorescein‑conjugated Monoclonal Antibody (Catalog # IC201F) and Mouse Anti-Human CD14 PE‑conjugated Monoclonal Antibody (Catalog # FAB3832P). Excess Recombinant Human IL‑1 beta /IL‑1F2 (Catalog # 201-LB) inhibits IC201F staining (B). Quadrant markers were set based on control antibody staining (Catalog # IC002F). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-1 beta/IL-1F2

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R Accessory Protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1 - 4). The human IL-1 beta cDNA encodes a 269 aa precursor. A 116 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5-7). The 17 kDa mature human IL-1 beta shares 96% aa sequence identity with rhesus and 67-78% with canine, cotton rat, equine, feline, mouse, porcine, and rat IL-1 beta.

  • References:
    1. Allan, S.M. et al. (2005) Nat. Rev. Immunol. 5:629.
    2. Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.
    3. Kornman, K.S. (2006) Am. J. Clin. Nutr. 83:475S.
    4. Isoda, K. and F. Ohsuzu (2006) J. Atheroscler. Thromb. 13:21.
    5. March, C.J. et al. (1985) Nature 315:641.
    6. Auron, P.E. et al. (1984) Proc. Natl. Acad. Sci. USA 81:7907.
    7. Martinon, F. and J. Tschopp (2007) Cell Death Differ. 14:10.
  • Long Name:
    Interleukin 1 beta
  • Entrez Gene IDs:
    3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)
  • Alternate Names:
    catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1B; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta
Related Research Areas

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

Showing Results 1 - 4 of 4
Filter your results:

Sample Type
  1. Kinin receptor expression during Staphylococcus aureus infection.
    Authors: Bengtson SH, Phagoo SB, Norrby-Teglund A, Pahlman L, Morgelin M, Zuraw BL, Leeb-Lundberg LM, Herwald H
    Blood, 2006;108(6):2055-63.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Nucleoporation of dendritic cells: efficient gene transfer by electroporation into human monocyte-derived dendritic cells.
    Authors: Lenz P, Bacot SM, Frazier-Jessen MR, Feldman GM
    FEBS Lett., 2003;538(1):149-54.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. T lymphocyte-mediated changes in airway smooth muscle responsiveness are attributed to induced autocrine release and actions of IL-5 and IL-1beta.
    Authors: Hakonarson H, Whelan R, Leiter J, Kim C, Chen M, Campbell D, Grunstein MM
    J. Allergy Clin. Immunol., 2002;110(4):624-33.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  4. Genetic control of IL-1beta bioactivity through differential regulation of the IL-1 receptor antagonist.
    Authors: Vamvakopoulos J, Green C, Metcalfe S
    Eur. J. Immunol., 2002;32(10):2988-96.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Fluorescein-conjugated Antibody

Ctrl IC002F 9  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Permeabilization/Wash Buffer I (1X)

Flow FC005 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Flow Cytometry Fixation Buffer (1X)

Flow FC004 3
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Flow Cytometry Fixation & Permeabilization Buffer Kit I

Flow FC009
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Flow Cytometry Fixation/Permeabilization Buffer I (1X)

Flow FC007
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Human IL-1 beta /IL-1F2 Fluorescein-conjugated Antibody?

Submit a review and receive a $25 amazon gift card if you include an image - $10 Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Certificate of Analysis Lookup

The document you requested is not available online. Please enter the Catalog Number and Lot Number below to have a document emailed to you at the address provided

Certificate of Analysis Lookup

Research Areas of Interest
R&D Systems Guarantee


Customer Information