Human IL-1 beta /IL-1F2 Fluorescein-conjugated Antibody

Catalog # Availability Size / Price Qty
IC201F
Detection of IL‑1 beta /IL‑1F2 in Human Blood Monocytes by Flow Cytometry.
1 Image
Product Details
Citations (4)
FAQs
Supplemental Products
Reviews

Human IL-1 beta /IL-1F2 Fluorescein-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-1 beta /IL-1F2 in Western blots. Shows less than 5% cross-reactivity with recombinant mouse (rm) IL-1 beta /IL-1F2 and recombinant porcine IL-1 beta /IL-1F2 and no cross-reactivity with recombinant rat (rr) IL-1 beta /IL-1F2, rmIL‑1 alpha, recombinant human IL‑1ra, rmIL‑1ra, or rrIL‑1 alpha.
Source
Monoclonal Mouse IgG1 Clone # 8516
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human IL-1 beta /IL-1F2
Ala117-Ser269
Accession # P01584
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of IL‑1 beta /IL‑1F2 antibody in Human Blood Monocytes antibody by Flow Cytometry. View Larger

Detection of IL‑1 beta /IL‑1F2 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes treated with LPS were stained with (A) Mouse Anti-Human IL-1 beta /IL-1F2 Fluorescein-conjugated Monoclonal Antibody (Catalog # IC201F) and Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (Catalog # FAB3832P). Excess Recombinant Human IL-1 beta /IL-1F2 (Catalog # 201-LB) inhibits IC201F staining (B). Quadrant markers were set based on control antibody staining (Catalog # IC002F). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: IL-1 beta/IL-1F2

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2, IL1B), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. The 17 kDa molecular weight mature human IL-1 beta shares 96% aa sequence identity with rhesus and 67%-78% with canine, cotton rat, equine, feline, mouse, porcine, and rat IL-1 beta. IL-1 beta functions in a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases.

Long Name
Interleukin 1 beta
Entrez Gene IDs
3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)
Alternate Names
catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta

Product Datasheets

You must select a language.

x

Citations for Human IL-1 beta /IL-1F2 Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
Filter your results:

Filter by:

  1. Kinin receptor expression during Staphylococcus aureus infection.
    Authors: Bengtson SH, Phagoo SB, Norrby-Teglund A, Pahlman L, Morgelin M, Zuraw BL, Leeb-Lundberg LM, Herwald H
    Blood, 2006-05-30;108(6):2055-63.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Nucleoporation of dendritic cells: efficient gene transfer by electroporation into human monocyte-derived dendritic cells.
    Authors: Lenz P, Bacot SM, Frazier-Jessen MR, Feldman GM
    FEBS Lett., 2003-03-13;538(1):149-54.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Genetic control of IL-1beta bioactivity through differential regulation of the IL-1 receptor antagonist.
    Authors: Vamvakopoulos J, Green C, Metcalfe S
    Eur. J. Immunol., 2002-10-01;32(10):2988-96.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. T lymphocyte-mediated changes in airway smooth muscle responsiveness are attributed to induced autocrine release and actions of IL-5 and IL-1beta.
    Authors: Hakonarson H, Whelan R, Leiter J, Kim C, Chen M, Campbell D, Grunstein MM
    J. Allergy Clin. Immunol., 2002-10-01;110(4):624-33.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human IL-1 beta /IL-1F2 Fluorescein-conjugated Antibody

There are currently no reviews for this product. Be the first to review Human IL-1 beta /IL-1F2 Fluorescein-conjugated Antibody and earn rewards!

Have you used Human IL-1 beta /IL-1F2 Fluorescein-conjugated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review