Human IL-1 beta /IL-1F2 Antibody MAB201-100: R&D Systems

Human IL-1 beta /IL-1F2 Antibody

(19 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human IL-1 beta /IL-1F2 in Western blots. Shows less than 5% cross-reactivity with recombinant mouse (rm) IL‑1 beta and rpIL‑1 beta and no cross-reactivity with rrIL‑1 beta, rmIL‑1 alpha, rhIL‑1ra, rmIL‑1ra, or rrIL‑1 alpha.
  • Source
    Monoclonal Mouse IgG1 Clone # 8516
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human IL-1 beta /IL-1F2
    aa 117-269
    Accession # P01584
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    8-25 µg/mL
    See below
  • Intracellular Staining by Flow Cytometry
    2.5 µg/106 cells
    Human peripheral blood mononuclear cells treated with LPS, fixed with paraformaldehyde, and permeabilized with saponin
  • Neutralization
    Measured by its ability to neutralize IL‑1 beta /IL‑1F2-induced proliferation in the D10.G4.1 mouse helper T cell line. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The Neutralization Dose (ND50) is typically 0.001-0.003 µg/mL in the presence of 50 pg/mL Recombinant Human IL‑1 beta /IL‑1F2 and 1.25 µg/mL concanavalin A.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples

Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutralization by Human
IL‑1 beta /IL‑1F2 Antibody.
Recombinant Human
IL‑1 beta /IL‑1F2 (Catalog # 201-LB) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑1 beta /IL‑1F2 (50 pg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB201). The ND50 is typically 0.001-0.003 µg/mL in the presence of concanavalin A (1.25 µg/mL).

Detection of Human IL‑1 beta /IL‑1F2 by Western Blot. Western blot shows lysates of THP‑1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 200 nM PMA for 24 hours and 10 µg/mL LPS and 3 hours. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB201) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IL‑1 beta /IL‑1F2 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

IL‑1 beta /IL‑1F2 in Human PBMCs.
IL‑1 beta /IL‑1F2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Human IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB201) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunocytochemistry

IL‑1 beta /IL‑1F2 in Human PBMCs. IL‑1 beta /IL‑1F2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) treated with LPS and monensin using Human IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB201) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-1 beta/IL-1F2

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1 - 4). The human IL-1 beta cDNA encodes a 269 aa precursor. A 116 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5-7). The 17 kDa mature human IL-1 beta shares 96% aa sequence identity with rhesus and 67-78% with canine, cotton rat, equine, feline, mouse, porcine, and rat IL-1 beta.

  • References:
    1. Allan, S.M. et al. (2005) Nat. Rev. Immunol. 5:629.
    2. Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.
    3. Kornman, K.S. (2006) Am. J. Clin. Nutr. 83:475S.
    4. Isoda, K. and F. Ohsuzu (2006) J. Atheroscler. Thromb. 13:21.
    5. March, C.J. et al. (1985) Nature 315:641.
    6. Auron, P.E. et al. (1984) Proc. Natl. Acad. Sci. USA 81:7907.
    7. Martinon, F. and J. Tschopp (2007) Cell Death Differ. 14:10.
  • Long Name:
    Interleukin 1 beta
  • Entrez Gene IDs:
    3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)
  • Alternate Names:
    catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

19 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Processing and secretion of guanylate binding protein-1 depend on inflammatory caspase activity
    Authors: E Naschberge, W Gei beta dörfer, C Bogdan, P Tripal, E Kremmer, M Stürzl, N Britzen-La
    J. Cell. Mol. Med, 2017;0(0):.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  2. Opposing effects of Nrf2 and Nrf2-activating compounds on the NLRP3 inflammasome independent of Nrf2-mediated gene expression
    Authors: M Garstkiewi, GE Strittmatt, S Grossi, J Sand, G Fenini, S Werner, LE French, HD Beer
    Eur. J. Immunol, 2017;0(0):.
  3. Adipose progenitor cell secretion of GM-CSF and MMP9 promotes a stromal and immunological microenvironment that supports breast cancer progression
    Authors: F Reggiani, V Labanca, P Mancuso, C Rabascio, G Talarico, S Orecchioni, A Manconi, F Bertolini
    Cancer Res., 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  4. NLRP3 inflammasome: a novel link between lipoproteins and atherosclerosis
    Arch Med Sci, 2016;12(5):950-958.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: WB
  5. Differential IL-1? secretion by monocyte subsets is regulated by Hsp27 through modulating mRNA stability
    Sci Rep, 2016;6(0):39035.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  6. Periodontitis-associated pathogens P. gingivalis and A. actinomycetemcomitans activate human CD14(+) monocytes leading to enhanced Th17/IL-17 responses
    Authors: Wan-Chien Cheng
    Eur J Immunol, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  7. Development of a Novel Model for the Assessment of Dead-Space Management in Soft Tissue.
    Authors: Oliver R, Lovric V, Yu Y, Christou C, Aiken S, Cooper J, Walsh W
    PLoS ONE, 2015;10(8):e0136514.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  8. Herpesvirus Genome Recognition Induced Acetylation of Nuclear IFI16 Is Essential for Its Cytoplasmic Translocation, Inflammasome and IFN-beta Responses.
    Authors: Ansari M, Dutta S, Veettil M, Dutta D, Iqbal J, Kumar B, Roy A, Chikoti L, Singh V, Chandran B
    PLoS Pathog, 2015;11(7):e1005019.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: WB
  9. Neutrophil elastase promotes interleukin-1beta secretion from human coronary endothelium.
    Authors: Alfaidi M, Wilson H, Daigneault M, Burnett A, Ridger V, Chamberlain J, Francis S
    J Biol Chem, 2015;290(40):24067-78.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  10. Pathogen-induced human TH17 cells produce IFN-gamma or IL-10 and are regulated by IL-1beta.
    Authors: Zielinski CE, Mele F, Aschenbrenner D, Jarrossay D, Ronchi F, Gattorno M, Monticelli S, Lanzavecchia A, Sallusto F
    Nature, 2012;484(7395):514-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  11. Monocytes Induce STAT3 Activation in Human Mesenchymal Stem Cells to Promote Osteoblast Formation.
    Authors: Nicolaidou V, Wong MM, Redpath AN
    PLoS ONE, 2012;7(7):e39871.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  12. IL-26 Is Overexpressed in Rheumatoid Arthritis and Induces Proinflammatory Cytokine Production and Th17 Cell Generation.
    Authors: Corvaisier M, Delneste Y, Jeanvoine H, Preisser L, Blanchard S, Garo E, Hoppe E, Barre B, Audran M, Bouvard B, Saint-Andre J, Jeannin P
    PLoS Biol, 2012;10(9):e1001395.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  13. TLR2-activated human langerhans cells promote Th17 polarization via IL-1beta, TGF-beta and IL-23.
    Authors: Aliahmadi E, Gramlich R, Grutzkau A, Hitzler M, Kruger M, Baumgrass R, Schreiner M, Wittig B, Wanner R, Peiser M
    Eur. J. Immunol., 2009;39(5):1221-30.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  14. Flightless-I regulates proinflammatory caspases by selectively modulating intracellular localization and caspase activity.
    Authors: Li J, Yin HL, Yuan J
    J. Cell Biol., 2008;181(2):321-33.
    Species: Human
    Sample Type: Whole Cells
    Application: WB
  15. Inflammatory cytokines stimulate the adhesion of colon carcinoma cells to mesothelial monolayers.
    Authors: van Grevenstein WM, Hofland LJ, van Rossen ME, van Koetsveld PM, Jeekel J, van Eijck CH
    Dig. Dis. Sci., 2007;52(10):2775-83.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  16. Interleukins 1beta and 6 but not transforming growth factor-beta are essential for the differentiation of interleukin 17-producing human T helper cells.
    Authors: Acosta-Rodriguez EV, Napolitani G, Lanzavecchia A, Sallusto F
    Nat. Immunol., 2007;8(9):942-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  17. Neutrophil-derived elastase induces TGF-beta1 secretion in human airway smooth muscle via NF-kappaB pathway.
    Authors: Lee KY, Ho SC, Lin HC, Lin SM, Liu CY, Huang CD, Wang CH, Chung KF, Kuo HP
    Am. J. Respir. Cell Mol. Biol., 2006;35(4):407-14.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  18. Intravenous human interleukin-1alpha impairs memory processing in mice: dependence on blood-brain barrier transport into posterior division of the septum.
    Authors: Banks WA, Farr SA, La Scola ME
    J. Pharmacol. Exp. Ther., 2001;299(2):536-41.
    Species: Human
    Sample Type: Recombinant Protein
    Application: Neut
  19. Amyloid beta and amylin fibrils induce increases in proinflammatory cytokine and chemokine production by THP-1 cells and murine microglia.
    Authors: Yates SL, Burgess LH, Kocsis-Angle J, Antal JM, Dority MD, Embury PB, Piotrkowski AM, Brunden KR
    J. Neurochem., 2000;74(3):1017-25.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
Expand to show all 19 Citations
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