Human IL-32 DuoSet ELISA

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Ancillary Products Available
Human IL-32 ELISA Standard Curve
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Product Details
Citations (7)
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Human IL-32 DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
96-well strip plate
Sample Volume Required
100 µL
Assay Range
78.1 - 5,000 pg/mL
Sufficient Materials
For five 96-well plates*
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human IL-32. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Scientific Data

Human IL-32 ELISA Standard Curve

Product Datasheets

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Preparation and Storage

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-32

IL-32 alpha is the shortest and most abundant of four potential splice variants of the proinflammatory cytokine IL-32 (previously called NK4) with a predicted unmodified size of 15 kDa. Potential modifications include myristoylation and N-glycosylation. Transfected IL-32 alpha was more likely to be cell-associated as compared to IL-32 beta, suggesting an intracellular function. IL-32 is induced by mitogens in peripheral lymphocytes, by IFN-gamma in epithelial cells, or by IL-12 with IL-18 in NK cells and in turn induces cytokine expression. No ortholog has been found in mouse.

Long Name:
Interleukin 32
Entrez Gene IDs:
9235 (Human)
Alternate Names:
IL32; IL-32; IL-32alpha; IL-32beta; interleukin 32; interleukin-32 theta; interleukin-32; natural killer cell transcript 4; Natural killer cells protein 4; NK4; NK4IL-32delta; TAIF; TAIFa; TAIFb; TAIFc; TAIFd; TAIFIL-32gamma; Tumor necrosis factor alpha-inducing factor

Citations for Human IL-32 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Circulating Interlukin-32 and Altered Blood Pressure Control in Individuals with Metabolic Dysfunction
    Authors: M Tomasi, A Cherubini, S Pelusi, S Margarita, C Bianco, F Malvestiti, L Miano, S Romeo, D Prati, L Valenti
    International Journal of Molecular Sciences, 2023-04-18;24(8):.
    Species: Human
    Sample Types: Plasma
  2. Integrated Analysis Revealed an Inflammatory Cancer-Associated Fibroblast-Based Subtypes with Promising Implications in Predicting the Prognosis and Immunotherapeutic Response of Bladder Cancer Patients
    Authors: H Chen, W Yang, X Xue, Y Li, Z Jin, Z Ji
    International Journal of Molecular Sciences, 2022-12-15;23(24):.
    Species: Human
    Sample Types: Cell Culture Supernates
  3. Interleukin-32gamma in the Control of Acute Experimental Chagas Disease
    Authors: YLL Braga, JRC Neto, AWF Costa, MVT Silva, MV Silva, MRN Celes, MAP Oliveira, LAB Joosten, F Ribeiro-Di, RS Gomes, JR Machado
    Journal of Immunology Research, 2022-01-20;2022(0):7070301.
    Species: Mouse
    Sample Types: Tissue Homogenates
  4. β-Glucan-Induced Trained Immunity Protects against Leishmania braziliensis Infection: a Crucial Role for IL-32
    Authors: JC Dos Santos, AM Barroso de, MV Teodoro Si, B Cirovic, LCJ de Bree, MSMA Damen, SJCFM Moorlag, RS Gomes, MM Helsen, M Oosting, ST Keating, A Schlitzer, MG Netea, F Ribeiro-Di, LAB Joosten
    Cell Rep, 2019-09-03;28(10):2659-2672.e6.
    Species: Human, Transgenic Mouse
    Sample Types: Cell Culture Supernates, Tissue Homogenates
  5. Serum levels of IL-32 in patients with type 2 diabetes mellitus and its relationship with TNF-α and IL-6
    Authors: R Fadaei, N Bagheri, E Heidarian, A Nouri, Z Hesari, N Moradi, A Ahmadi, R Ahmadi
    Cytokine, 2019-08-31;125(0):154832.
    Species: Human
    Sample Types: Serum
  6. A Model Based on the Combination of IFN-gamma, IP-10, Ferritin and 25-Hydroxyvitamin D for Discriminating Latent From Active Tuberculosis in Children
    Authors: P Comella-De, R Abellana, R Villar-Her, MD Jean Coute, B Sallés Min, L Canales Al, M Narcisse, J Gautier, C Ascaso, I Latorre, J Dominguez, TM Perez-Porc
    Front Microbiol, 2019-08-14;10(0):1855.
    Species: Human
    Sample Types: Plasma
  7. Resistin upregulates chemokine production by fibroblast-like synoviocytes from patients with rheumatoid arthritis
    Authors: H Sato, S Muraoka, N Kusunoki, S Masuoka, S Yamada, H Ogasawara, T Imai, Y Akasaka, N Tochigi, H Takahashi, K Tsuchiya, S Kawai, T Nanki
    Arthritis Res. Ther., 2017-12-01;19(1):263.
    Species: Human
    Sample Types: Cell Culture Supernates


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