Human IL-32 alpha Antibody

Catalog # Availability Size / Price Qty
MAB30401
MAB30401-SP
Detection of Human IL‑32 alpha  by Western Blot.
2 Images
Product Details
Citations (3)
FAQs
Supplemental Products
Reviews (1)

Human IL-32 alpha Antibody Summary

Species Reactivity
Human
Specificity
Detects the alpha isoform of IL-32 in direct ELISAs. In direct ELISAs, less than 15% cross-reactivity with recombinant human (rh) IL-32 beta and rhIL‑32 gamma is observed.
Source
Monoclonal Rat IgG2A Clone # 373802
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human IL‑32 alpha
Phe3-Lys131
Accession # NP_001012651
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human IL-32a antibody by Western Blot. View Larger

Detection of Human IL‑32 alpha by Western Blot. Western blot shows lysates of human peripheral blood mononuclear cells (PBMC) treated (+) with 200 ng/mL Ionomycin and 10 ng/mL PMA for 72 hours. PVDF Membrane was probed with 2 µg/mL of Human IL-32a Monoclonal Antibody (Catalog # MAB30401) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for IL-32a at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry IL-32a antibody in Human PBMCs by Immunocytochemistry (ICC). View Larger

IL‑32 alpha in Human PBMCs. IL-32a was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Human IL-32a Monoclonal Antibody (Catalog # MAB30401) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (yellow; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-32 alpha

IL-32 alpha is the shortest and most abundant of four potential splice variants of the proinflammatory cytokine IL-32 (previously called NK4) with a predicted unmodified size of 15 kDa. Potential modifications include myristoylation and N-glycosylation. Transfected IL-32 alpha was more likely to be cell-associated as compared to IL-32 beta, suggesting an intracellular function. IL-32 is induced by mitogens in peripheral lymphocytes, by IFN‑ gamma in epithelial cells, or by IL-12 with IL-18 in NK cells and in turn Induces cytokine expression. No ortholog has been found in mouse.

Long Name
Interleukin 32 alpha
Entrez Gene IDs
9235 (Human)
Alternate Names
IL32 alpha; IL-32 alpha; IL32; IL-32alpha; IL-32beta; IL-32delta; IL-32gamma; interleukin 32; NK4; TAIF; TAIFa; TAIFb; TAIFc; TAIFd

Product Datasheets

Citations for Human IL-32 alpha Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Allele-specific long-distance regulation dictates IL-32 isoform switching and mediates susceptibility to HIV-1
    Authors: RJ Palstra, E de Crignis, MD Röling, T van Staver, TW Kan, W van Ijcken, YM Mueller, PD Katsikis, T Mahmoudi
    Sci Adv, 2018;4(2):e1701729.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Increased Interleukin-32 Levels in Obesity Promote Adipose Tissue Inflammation and Extracellular Matrix Remodeling: Effect of Weight Loss
    Authors: Victoria Catalán
    Diabetes, 2016;65(12):3636-3648.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  3. Native IL-32 is released from intestinal epithelial cells via a non-classical secretory pathway as a membrane-associated protein.
    Authors: Hasegawa H, Thomas HJ, Schooley K, Born TL
    Cytokine, 2011;53(1):74-83.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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Reviews for Human IL-32 alpha Antibody

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Human IL-32 alpha Antibody
By Anonymous on 03/08/2019
Application: ICC/IF Sample Tested: Adult lung Species: Human

Did not work with human lung sections without retreival or with citrate retreival - IgG was used as control.
***Bio-Techne Response: Thank you for reviewing our product. We are sorry to hear that this antibody did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.***